Investigation of role of MafB for tumor immunity

利用巨噬细胞特异性表达显性阴性（DN） MafB的小鼠，研究了巨噬细胞转录因子MafB在肿瘤免疫中的作用。B16-F10黑色素瘤细胞从小鼠尾静脉注射到小鼠体内。野生型小鼠与DN-MafB转基因小鼠（TG）的存活率无显著差异。两组间肿瘤内及肿瘤周围巨噬细胞数量无明显差异。两组未治疗小鼠的存活率相似。此外，在腹腔注射氨基甲酸乙酯6个月后，测量肺肿瘤的大小，如结节和增生病变。TG与野生型小鼠肺结节大小及增生性病变均无显著差异。本研究揭示了转录因子MafB在小鼠肿瘤免疫中并不起重要作用。

Role of macrophage transcription factor MafB for tumor immunity was investigated using mice that specifically express dominant negative (DN) MafB in macrophages. B16-F10 melanoma cells were administered to the mice from tail vein. Survival rate was not significantly different between wild type mice and DN-MafB transgenic mice (TG). The number of macrophages in tumors and those around tumors were not different between two groups. Survival rate of untreated mice were similar in both groups. In addition, 6 months after intraperitoneal urethane treatment, the sizes of lung tumors, such as nodules and hyperplasia lesions, were measured. Both sizes of lung nodules and hyperplastic lesions were not significantly different between TG and wild type mice. This study revealed that transcription factor MafB does not play important roles for tumor immunity in mice.