Analysis of antisense RNA and development of new diagnostics of gastrointestinal cancer using blood.

本研究的目的是对反义RNA进行分析，建立利用血液诊断胃肠道肿瘤的新方法。我们在结直肠癌患者的血液中鉴定了20个随健康人变化的反义RNA。这些反义RNA可以清楚地区分健康人和结肠癌患者。我们还分别鉴定了15个miRNA和17个miRNA，分别在结肠癌患者和胰腺癌患者的血液中转化为健康人的外切体。这些miRNA可以清晰地区分健康人和结直肠癌患者。根据这些结果，我们将开发新的低成本定制微阵列，用于胃肠道癌症的诊断。

The aim of this research is to analyze of antisense RNA and develop of new diagnostics of gastrointestinal cancer using blood.We identified 20 anti-sense RNA that is changing with the healthy people in the blood of patients with colorectal cancer. These antisenseRNA was possible to clearly distinguish the healthy people and colon cancer patients. We also identified 15 miRNA and 17 miRNA, respectively, changing to healthy people in the blood of exosomes in patients with colon cancer and pancreatic cancer patients. These miRNA was possible to distinguish clearly healthy people and patients with colorectal cancer.From these results, we will develop new low cost custom microarray for diagnosis of gastrointestinal cancer.