Cell culture laboratory and animal facility for the cell and animal based projects of the Forschungsgruppe 449

用于研究组 449 细胞和动物项目的细胞培养实验室和动物设施

• 批准号: 5434403
• 负责人: Professor Dr. Ingo Birger Autenrieth
• 金额: --
• 依托单位: Universitätsklinikum Heidelberg
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5434403?language=en
• 关键词: Cell; culture; laboratory; animal; facility

Several projects of the Forschergruppe are dealing with host-pathogen interactions. To obtain optimized cooperations and synergistic effects whithin the Forschergruppe, cultivation of mammalian cells for cellular microbiology experiments of the Forschergruppe will be performed in a common cell culture facility at the Instiute for Medical Microbiology. Cell culture of, e.g., epithelial cells (HeLa), endothelial cells (HUVECs), macrophages, dendritic cells or fibroblasts will be performed according to highly standardized protocols in this central cell culture laboratory. Additionally, cell infection experiments, preparation of primary cells and preparing cell stocks including management of the cell stocks will be performed in this central unit. This facility will ensure a high level of reproducibility of cell culture experiments. Likewise a central animal facility at the Institute of Medical Mirobiology provides excellent specific-pathogen free animal housing conditions with IVCs and a number of gene-targeted mouse strains that can be used by the various projects of the Forschergruppe for in vitro and in vivo experiments to study the interactions of bacterial cell envelope and the host.

研究小组的几个项目涉及宿主-病原体相互作用。为了获得最佳的合作和协同效应，将在医学微生物学研究所的普通细胞培养设施中培养用于研究组细胞微生物学实验的哺乳动物细胞。细胞培养物，例如，上皮细胞（HeLa）、内皮细胞（HUVEC）、巨噬细胞、树突状细胞或成纤维细胞的培养将根据该中心细胞培养实验室的高度标准化方案进行。此外，细胞感染实验、原代细胞制备和细胞储备液制备（包括细胞储备液管理）将在该中心单元中进行。该设施将确保细胞培养实验的高水平再现性。同样，医学微生物学研究所的一个中心动物设施为IVC和许多基因靶向小鼠品系提供了极好的无特定病原体的动物饲养条件，这些小鼠品系可用于研究小组的各种项目进行体外和体内实验，以研究细菌细胞包膜和宿主的相互作用。