Expression of "Imprinting"memory in chick brain

“印记”记忆在鸡脑中的表达

• 批准号: 09680790
• 负责人: MATSUSHIMA Toshiya
• 金额: $ 2.11万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680790/
• 关键词: brain; memory; imprinting; basal ganglion; synapse; long-term potentiation; dopamine; bird; インプリンティング; 行動; 刷り込み; 最初期 遺伝子; 可塑性

1. Behavioral study on the memory contents formed after visual discrimination taskNewly hatched chicks learn to discriminate what is edible and what is not by experiences. Single experience of pecking at a aversive object results in a formation of long-term memory ; a task refered to as "one-trial passive avoidance task." Detailed analysis of chick perceptual map formed after this task revealed that chicks process "color" and "shape" separately, the former deteriorates earlier than the latter.2. Single-unit analysis on the expression of visual images in association area of chick brainWe have developed a technique, where activity of single neurons can be recorded for hours from brain (visual association area and basal ganglion) of freely-behaving awake chicks. Some neurons revealed characteristic coherent bursts immediately after the formation of the passive avoidance memory.3. Neurophysiological study on the synaptic plasticity in basal gangion ; concurrent induction of long-term potentiation and depression of converging synaptic inputs.The basal gangion (area LPO) is assumed the brain region where the passive avoidance memory is stored for selective execution of pecking behavior. Field potential and whole cell patch recording from the LPO neurons in slices revealed convergence of excitatory inputs. Coincident tetanic stimuli caused a LTP in "cotrico-striatal" inputs and a concomittant LTD in the "amgdalo-striatal" inputs.

1.视觉辨别后形成的记忆内容的行为学研究初孵雏鸡通过经验学习辨别什么是可食的，什么是不可食的。单次啄厌恶性物体的经验导致长时记忆的形成，这一任务被称为“一次性被动回避任务”。通过对实验后形成的雏鸡知觉图的详细分析，发现雏鸡对“颜色”和“形状”的加工是分开的，前者的劣化早于后者.鸡脑联合区视觉图像表达的单单位分析我们开发了一种技术，可以记录自由行为清醒的鸡脑（视觉联合区和基底神经节）单个神经元的活动数小时。部分神经元在被动回避记忆形成后立即出现特征性的连贯爆发.基底神经节突触可塑性的神经生理学研究：长时程增强和突触会聚输入抑制的同时诱导。基底神经节（LPO区）被认为是储存被动回避记忆的脑区，用于选择性执行啄食行为。场电位和全细胞片记录的LPO神经元在切片显示兴奋性输入的收敛。同时强直刺激引起的LTP在“cotrico-striatal”的输入和伴随的LTD在“amgdalo-striatal”的输入。

项目成果

Yazaki, Y., Matsushima, T., and Aoki, K.: "Testosterone modulates calling behavior in Japanese quail chicks." Zoological Science. 14. 219-225 (1997)

Yazaki, Y.、Matsushima, T. 和 Aoki, K.：“睾酮调节日本鹌鹑雏鸟的叫声行为。”

Yazaki,Y.,Matsushima,T.,and Aoki: "Stimulation elicits the chick crowing with testosterone in Japanese quail chicks." Zoological Science. 14. 227-231 (1997)

Yazaki, Y.、Matsushima, T. 和 Aoki：“刺激会引起日本鹌鹑雏鸡体内含有睾酮的鸡鸣。”

Yanagihara,S.,and Matsushima,T.: "Enhanced expression of ZENK after passive avoidance task and its relevance to memory formation in quail chicks." Society for Neuroscience(Abstract). 25. 1422-560.12 (1998)

Yanagihara, S. 和 Matsushima, T.：“被动回避任务后 ZENK 的表达增强及其与鹌鹑雏鸡记忆形成的相关性。”

Takeuchi, H,-A., Yazaki, Y., Mtsushima, T.and Aoki, A.: "Expression of Fos-like immunoreactivity in the brain of quail chick emitting the isolation-induced distress calls." Neuroscience Letters. 220. 191-194 (1996)

Takeuchi, H,-A.、Yazaki, Y.、Mtsushima, T. 和 Aoki, A.：“发出隔离引起的求救呼叫的鹌鹑雏鸡大脑中 Fos 样免疫反应的表达。”

Takeuchi,H.-A.,Yazaki,Y.,Matsushima,T.and Aoki,A.: "Expression of Fos-like immunoreactivity in the brain of quail chick emitting the isolation-induced distress calls." Neuroscience Letters. 220. 191-194 (1996)

Takeuchi,H.-A.、Yazaki,Y.、Matsushima,T. 和 Aoki,A.：“发出隔离引起的求救呼叫的鹌鹑雏鸡大脑中 Fos 样免疫反应的表达。”