Long-term potentiation of synaptic transmission in hyperstriatum of neonatal chicks : Mechanisms of "Imprinting"

新生雏鸡上纹状体突触传递的长期增强：“印记”机制

基本信息

批准号：
07680882
负责人：
MATSUSHIMA Toshiya
金额：
$ 1.41万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680882/
关键词：
Brain Memory Behavior Imprinting Immediate early gene Synapse Long-term Potentiation 長期増強発達学習

项目摘要

(1) Behavioral studies on habituation and reinforced learning in the feeding behavior.Neonatal chicks peck at small and conspicuous objects. This action is innate and not requiring postnatal experiences, which however drastically change the preferences of the pecking action. In this study, habituation to neutral stimuli and reinforced one-trial learning were examined in this study.The pecking action showed a strong habituation for stimulus object that was repeatedly presented, irrespectively of where the interval was short (5min) or long (1 hour). The habituated response was not observed when an object of the same size, shape but of sifferenent color was presented, indicating that the memory content was mainly its color. The passive avoidance learning reinforced by aversive taste, on the other hand, was formed after a single experience of pecking a bitter object, and the accompanying memory content covered not only the color, but also its shape and procedure of the object presentation. … More The same object could thus be stored in different manners in the central nervous system, and the neural mechanisms underlying these two forms of memory should be analyzed.(2) Neurological studies on causal relationships between immediate early gene (c-fos) and LTP.Expression of immediate early gene (c-fos) in a hyperstriatal region (IMHV) has been shown to correlate significantly with the strength of learming accomplished in the imprinting paradigm (McCabe and Horn 1994). Long-term potentiation (LTP) of synaptic transmission, on the other hand, has been assumed to constitute the cellular basis of memory formation (Matsushima and Aoki 1995). In this study, the causal relationships between the c-fos expression and LTP has been analyzed.Experimental condition sufficient for inducing LTP in the local synaptic transmission (5Hzx300pulses) failed to enhance the c-fos expression. Conversely, those slices where endogenous expression of c-fos was high, the conditioning electrical stimulation failed to induce LTP.Furthermore, an antagonist of GABA-A receptors (biuculline) locked the LTP,while the c-fos expression was somewhat enhanced.Both of the c-fos expression and the synaptic LTP are plastic process which depend on the preceding neural activities, however, the underlying cellular mechanisms proved to be distinct. Rather, the c-fos expression tended to negatively correlated with the LTP.The molecular cascades for these processes remains to be unraveled, particularly in relation to the sensitive period of the filial learning. Less

（1）关于喂养行为中习惯和加强学习的行为研究。神经小鸡在小而明显的物体上啄。这一行动是天生的，不需要产后经验，无论从而极大地改变了啄食动作的偏好。在这项研究中，在这项研究中检查了对中性刺激和加强一试的一试学习的习惯。啄食的作用显示了刺激物体的强烈习惯，无论间隔短（5分钟）或长（1小时），刺激物体反复出现。当出现相同尺寸，形状但单独颜色的对象时，没有观察到习惯的响应，表明记忆含量主要是其颜色。另一方面，在啄食苦味物体的单一体验后，形成了因厌恶味而增强的被动回避学习，而参与的记忆内容不仅涵盖了颜色，而且涵盖了对象表现的形状和过程。 ……因此，更多的物体可以在中枢神经系统中以不同的方式存储，并且应分析这两种形式的记忆形式的中性机制。（2）关于立即的早期基因（C-FOS）和LTP的因果关系的神经学研究（C-FOS）在近气（C-FOS（C-FOS）（C-FOS）表达（C-FOS）（C-FOS）中的紧张（C-FOS）的强度（IMMHV）的强度（IM IMHV）的强度是相关的。范式（McCabe and Horn 1994）。另一方面，已经假定突触传播的长期增强（LTP）构成了记忆形成的细胞基础（Matsushima and Aoki 1995）。在这项研究中，已经分析了C-FOS表达与LTP之间的因果关系。实验条件足以在局部突触传播（5Hzx300Pulses）中诱导LTP，无法增强C-FOS表达。相反，那些C-FOS内源性表达的切片很高，调理电气模拟未能诱导LTP.Furthermore，GABA-A接收器的拮抗剂（Biuculline）锁定了LTP，而C-FOS表达的表达则有些增强。事实证明，机制是不同的。较少的