权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Helicobacter pylori and its association with gastric cancer

幽门螺杆菌及其与胃癌的关系

基本信息

批准号：
09670502
负责人：
TAKEDA Hiroshi
金额：
$ 1.92万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670502/
关键词：
MIF H.pylori parital cell

项目摘要

Macrophage migration inhibitory factor (MIF) is the first discovered lymphokine. it was originally identified by its ability to prevent the migration of macrophages out of capillary tubes. Since then, the expression of MIF activities has been found at a variety of inflammatory loci, suggesting its role in regulating the function of macrophages in host defence. More recent reports showed that MIF may have functions besides in the immune system. For instance, MIF mRNA and protein are expressed in anterior pituitary cells, brain, embryonic eye lens and differentiating epidermal cells, suggesting its pivotal role in the regulation of neuroendocrine system, cell growth and differentiation. Furthermore, it is possible that MIF has a role in the gastric mucosal inflammation caused by Helicobacter pylon (HP) infection and the development of gastric cancer in an inflamed gatric mcosa The aim of this study was to elucidatee the role of MIF in the patliogenesis of gastric diseases associated with … More HP infectionFirst, we examined the distribution of MIF in the gastrointestinal tract. MIF mRNA and protein were strongly expressed in the rat gastric mucoca and to a lesser extent in the oesophagus, small intestine and colon by Northern blot analysis and immunoblotting. By immunohistochemical study, positive MIF staining was confined within the cytoplasm of parietal cells. Chief cells and surface mucous cells were negative for MIF staining. Intraperitoneal injection of rat gastrin I caused a decline of MIF mRNA level. After 6 hours of injection, the level of MIF mRNA returned to the level before injection. These results suggest that MIF may play an important role in parietal cell physiology. In human gastric mucosa infected with HP, MIF was strongly expressed in infiltrating lymphocytes as well as gastric parietal cells. Finally, we examined the bood level of MIF before and after the eradication therapy for HP infection. We found that the serum MIF concentration in patients with HP infection was lower than that of HP negative control subjects, and after successful eradication therapy, MIF concentration raised to the level comparable to that of control subjects. These results suggest that the local inflammatory response to HP may have the profound influence on the immune system. Less

巨噬细胞迁移抑制因子（Macrophage migration inhibitory factor， MIF）是第一个发现的淋巴因子。它最初是通过其阻止巨噬细胞从毛细血管中迁移的能力而确定的。此后，在多种炎症位点发现了MIF活性的表达，提示其在调节巨噬细胞宿主防御功能中的作用。最近的报道显示，MIF除了在免疫系统中还有其他功能。例如，MIF mRNA和蛋白在垂体前叶细胞、大脑、胚胎眼晶状体和分化的表皮细胞中均有表达，提示其在调节神经内分泌系统、细胞生长和分化中起关键作用。此外，MIF可能在幽门螺杆菌（HP）感染引起的胃粘膜炎症和炎症胃黏膜内胃癌的发展中起作用。本研究的目的是阐明MIF在与HP感染相关的胃疾病的病理发生中的作用。Northern blot和免疫印迹分析显示，MIF mRNA和蛋白在大鼠胃粘膜中有较强的表达，在食管、小肠和结肠中有较弱的表达。通过免疫组化研究，MIF阳性染色局限于壁细胞的细胞质内。主细胞和表面黏液细胞MIF染色均为阴性。腹腔注射胃泌素I可引起大鼠MIF mRNA水平下降。注射6 h后，MIF mRNA水平恢复到注射前水平。这些结果提示MIF可能在壁细胞生理中起重要作用。在HP感染的人胃粘膜中，MIF在浸润淋巴细胞和胃壁细胞中强烈表达。最后，我们检测了HP感染根除治疗前后血液中MIF的水平。我们发现HP感染患者的血清MIF浓度低于HP阴性对照组，在根除治疗成功后，MIF浓度上升到与对照组相当的水平。这些结果提示HP的局部炎症反应可能对免疫系统有深远的影响。少