Helicobacter pylori and its association with gastric cancer

巨噬细胞移动抑制因子（MIF）是最早发现的淋巴因子。它最初被鉴定为具有防止巨噬细胞移出毛细管的能力。从那时起，MIF活性的表达已被发现在各种炎症部位，表明其在调节宿主防御中的巨噬细胞功能中的作用。最近的报道表明，MIF可能具有免疫系统以外的功能。如在垂体前叶细胞、脑、胚胎眼透镜和分化中的表皮细胞中均有MIF mRNA和蛋白的表达，提示其在神经内分泌系统、细胞生长和分化的调节中起着重要作用。此外，MIF可能在幽门螺杆菌（HP）感染引起的胃粘膜炎症和炎症胃粘膜中胃癌的发生中起作用。本研究的目的是阐明MIF在与HP感染相关的胃疾病的发生中的作用。 关于我们 HP感染首先，我们检测了MIF在胃肠道中的分布。北方印迹分析和免疫印迹分析表明，大鼠胃粘膜中有大量MIF mRNA和蛋白表达，食管、小肠和结肠中有少量表达。免疫组化结果显示，MIF阳性染色局限于壁细胞胞浆内。主细胞和表面粘液细胞MIF染色阴性。腹腔注射大鼠胃泌素I可引起MIF mRNA水平下降。注射后6h，MIF mRNA水平恢复至注射前水平。这些结果表明，MIF可能在壁细胞生理学中发挥重要作用。在HP感染的人胃粘膜中，MIF在浸润的淋巴细胞和胃壁细胞中强烈表达。最后，我们检测了HP根除治疗前后MIF的水平。发现HP感染者血清MIF浓度低于HP阴性对照组，经根除治疗后，血清MIF浓度升高至与对照组相当的水平。提示HP引起的局部炎症反应可能对机体免疫系统产生深远的影响。少

Macrophage migration inhibitory factor (MIF) is the first discovered lymphokine. it was originally identified by its ability to prevent the migration of macrophages out of capillary tubes. Since then, the expression of MIF activities has been found at a variety of inflammatory loci, suggesting its role in regulating the function of macrophages in host defence. More recent reports showed that MIF may have functions besides in the immune system. For instance, MIF mRNA and protein are expressed in anterior pituitary cells, brain, embryonic eye lens and differentiating epidermal cells, suggesting its pivotal role in the regulation of neuroendocrine system, cell growth and differentiation. Furthermore, it is possible that MIF has a role in the gastric mucosal inflammation caused by Helicobacter pylon (HP) infection and the development of gastric cancer in an inflamed gatric mcosa The aim of this study was to elucidatee the role of MIF in the patliogenesis of gastric diseases associated with … More HP infectionFirst, we examined the distribution of MIF in the gastrointestinal tract. MIF mRNA and protein were strongly expressed in the rat gastric mucoca and to a lesser extent in the oesophagus, small intestine and colon by Northern blot analysis and immunoblotting. By immunohistochemical study, positive MIF staining was confined within the cytoplasm of parietal cells. Chief cells and surface mucous cells were negative for MIF staining. Intraperitoneal injection of rat gastrin I caused a decline of MIF mRNA level. After 6 hours of injection, the level of MIF mRNA returned to the level before injection. These results suggest that MIF may play an important role in parietal cell physiology. In human gastric mucosa infected with HP, MIF was strongly expressed in infiltrating lymphocytes as well as gastric parietal cells. Finally, we examined the bood level of MIF before and after the eradication therapy for HP infection. We found that the serum MIF concentration in patients with HP infection was lower than that of HP negative control subjects, and after successful eradication therapy, MIF concentration raised to the level comparable to that of control subjects. These results suggest that the local inflammatory response to HP may have the profound influence on the immune system. Less