ANALYSIS OF CYTOTOXIC MECHANISMS OF ACTIVATED EOSINOPHILS IN BILIARY CELL INJURY : ESTABLISHMENT OF AN ANIMAL MODEL FOR PRIMARY BILIARY CIRRHOSIS
胆道细胞损伤中活化的嗜酸性粒细胞的细胞毒机制分析:原发性胆汁性肝硬化动物模型的建立
基本信息
- 批准号:09670555
- 负责人:
- 金额:$ 1.66万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Activated eosinophilic infiltration is a common histological feature in diseased liver such as chronic rejection of transplanted liver, GVHD, or primary biliary cirrhosis (PBC) resembling chronic CVHD.In such conditions, it is suggested that activated eosinophils play an important role in liver damages, though precise mechanisms have not been analyzed. Using IL-5 transgenic (IL5Tg) mouse, we have first established an experimental model of liver injury caused by activated eosinophils.lL5Tg mice (C3H/HeN-TgN(IL-5)Imeq) have been established and provided from DR.Tominaga, Kochi Medical School. Although this mouse exhibits marked eosinophilia (30-70%) in the periphery, tissue injuries are not observed in any organs. IL5Tg mice were injected with 25 mug of LPS (Salmonella minesota Re 595) interaperitoneally and sacrificed 2 weeks later. Histological examinations showed marked portal inflammation with eosinophilic infiltrations (>50%) in portal tracts and extensive lobutar necrosis surround … More by marked eosinophils. Some small bile ducts were degenerated and destructed with eosinophihic aggregates, where eositiophilic infiltrations into ductal epithelium were observed. Electron microscopic examinations showed degranulation of eosinophilic cytotoxic granules into the ductal cells. Tissue injuries other than liver were almost absent in IL5Tg mice. in control mice (C3H/HeN), LPS injection did not induced any tissue injuries.Spleen cells (2x107 cells ; eosinophils>50%) from IL5Tg primed with LPS were transferred into none-transgenic mice (C3H/HeN) intravenously, but any tissue injuries were never induced. This result suggests that in addition to activated eosinophils, another factors such as various cytokines including TNF a released from LPS stimulated Kupffer cells are necessary for the induction of eosinophilic hepatic injury. Further analysis for dynamic changes of cytokines or chemokines, which are related to chemotaxis and degranulation of activated eosinophils, is needed for understanding the hepatic injury in this animal model. Less
嗜酸性粒细胞活化是慢性排斥反应、移植物抗宿主病(GVHD)、原发性胆汁性肝硬化(PBC)等肝脏疾病的常见病理特征,在这些疾病中,嗜酸性粒细胞活化在肝脏损伤中起重要作用,但其确切机制尚未明确。使用IL-5转基因(IL-5 Tg)小鼠,我们首先建立了由活化的嗜酸性粒细胞引起的肝损伤的实验模型。IL-5 Tg小鼠(C3 H/HeN-TgN(IL-5)Imeq)已经建立并由高知医学院的DR.Tominaga提供。尽管该小鼠在外周表现出明显的嗜酸性粒细胞增多(30-70%),但在任何器官中均未观察到组织损伤。IL 5 Tg小鼠腹膜内注射25 μ g LPS(Salmonella minesota Re 595),2周后处死。组织学检查显示明显的门静脉炎症伴汇管区嗜酸性粒细胞浸润(>50%)和广泛的小叶周围坏死 ...更多信息 嗜酸性粒细胞一些小胆管变性和破坏,嗜酸性粒细胞聚集,其中嗜酸性粒细胞浸润到导管上皮细胞中观察到。电子显微镜检查显示嗜酸性细胞毒性颗粒脱颗粒进入导管细胞。在IL 5 Tg小鼠中几乎不存在肝脏以外的组织损伤。将来自用LPS致敏的IL 5 Tg的脾细胞(2 × 107个细胞;嗜酸性粒细胞>50%)静脉内转移到非转基因小鼠(C3 H/HeN)中,但从未诱导任何组织损伤。这一结果表明,除了活化的嗜酸性粒细胞外,另一种因素如LPS刺激的枯否细胞释放的各种细胞因子包括TNF α是诱导嗜酸性肝损伤所必需的。进一步分析与活化嗜酸性粒细胞趋化和脱颗粒有关的细胞因子或趋化因子的动态变化,对于理解该动物模型中的肝损伤是必要的。少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MAEDA Takashi其他文献
人工知能により労働者のメンタルヘルス不調を精神科医よりも高精度で判定
人工智能比精神科医生更准确地确定工人的心理健康问题
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
SHIGEMURA Hiroaki;MAEDA Takashi;NAKAYAMA Shiko;OHISHI Akira;CARLE Yuki;OOKUMA Eiko;ETOH Yoshiki;HIRAI Shinichiro;MATSUI Mari;KIMURA Hirokazu;SEKIZUKA Tsuyoshi;KURODA Makoto;SERA Nobuyuki;INOSHIMA Yasuo;MURAKAMI Koichi;道喜将太郎; 笹原信一朗; 堀大介; 大井雄一; 髙橋司; 池田有; 池田朝彦; 新井陽; 室井慧; 松崎一葉 - 通讯作者:
道喜将太郎; 笹原信一朗; 堀大介; 大井雄一; 髙橋司; 池田有; 池田朝彦; 新井陽; 室井慧; 松崎一葉
MAEDA Takashi的其他文献
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{{ truncateString('MAEDA Takashi', 18)}}的其他基金
Identification of receptor for cartducin, and analysis of the role of cartducin in inflammation
Cartducin受体的鉴定及其在炎症中的作用分析
- 批准号:
18K09534 - 财政年份:2018
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
A Study of The Factors Influencing Learning Outcomes of Medical Students
医学生学习成果影响因素研究
- 批准号:
26780472 - 财政年份:2014
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Potential role of cartducin as a novel regulator of skeletal myogenic differentiation and maturation
Cartducin 作为骨骼肌原性分化和成熟的新型调节剂的潜在作用
- 批准号:
26462836 - 财政年份:2014
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study on Local Tax Autonomy in the Decentralized Fiscal System
分权财政体制下的地方税收自治研究
- 批准号:
23530396 - 财政年份:2011
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of Student Culture of Part-time Course in High School from The Reconstruction Period after World War II to The period of Economic High Growth in Japan
日本二战后重建时期到经济高速增长时期高中兼读制学生文化研究
- 批准号:
20830108 - 财政年份:2008
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Young Scientists (Start-up)
A Study on the Desirable Real Estate Tax as Main Local Tax in the Aged Society
老龄化社会理想的房地产税作为主要地方税的研究
- 批准号:
20530277 - 财政年份:2008
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the elevated expression of cartducin gene in osteosarcoma and the mechanisms of tumor development
骨肉瘤中cartducin基因表达升高及肿瘤发生机制分析
- 批准号:
20592197 - 财政年份:2008
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysi of the role of cartducin, a novel growth factor secreted from cartilage, on bone development
软骨分泌的新型生长因子cartducin对骨发育的作用分析
- 批准号:
18592062 - 财政年份:2006
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of a Collaborative e-Learning System and Educational Methods Based on Enquity-Based Learning and Adaptive Instruction Methodology
基于知识学习和适应性教学方法的协作电子学习系统和教育方法的开发
- 批准号:
17300271 - 财政年份:2005
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Am application of Kempf complex to hyper-discriminant
Kempf 复合体在超判别中的应用
- 批准号:
14540035 - 财政年份:2002
- 资助金额:
$ 1.66万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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The formation and degradation of neutrophil and eosinophil extracellular traps in otitis media
中耳炎中中性粒细胞和嗜酸性粒细胞胞外陷阱的形成和降解
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