The observation of rhizosphere to reflect the nutritional conditions by image analysis and the application of the method to diagnosis.

我们已经建立了一种观察rhizosphere的方法来反映营养条件，使用两种新的植物根箱制造的phenolic resin form，用于在水文化和图像分析中开发整个根系统。根框允许更好地可视化pH值中的变化以及图像分析在一个表示性的全根系统中的硝酸盐和磷酸盐的分布。pH值与pH指示器、硝酸盐和磷酸盐结合与颜色代理相结合，在根系统的配置文件上散布.我们观察到根系统的介质在根系统中生长1/4（NH-D24-N/ NO-D23-D-2-N）介质直到12 h，而在48 h时，pH玫瑰再次上升到6.0和更高。这一风险是由在24小时和48小时的硝酸盐集中分解引起的。在对比度下，随着时间的推移，中国小屋的pH值增加了，当硝酸盐减少时，建议在螺旋桨pH值变化和植物的首选温度/洋葱之间建立关系。此外，我们还观察到了与铁和磷缺乏有关的嗜氮细胞的酸性，以及通过共存的不相容磷酸盐（AlPO y D24 y D2和FePO y D24 y D2）。在添加中，我们根据图像分析中的根活动评估了根颜色在不同的肥料条件下的根活动。我们可以建立一种方法来应用于植物的营养判断。

We have established an observation method of rhizosphere to reflect the nutritional conditions using by both a new plant root box made of phenolic resin form for developing whole root systems in water culture and image analysis. The root box allowed better visualization of changes in pH and the distribution of nitrate and phosphorus by image analysis in a representative whole root system. The pH values were detected with pH indicators, nitrate and phosphorus concentration with color reagents, sprayed onto profiles of the root system.We observed acidification of the medium in the root system of wheat grown 1/4(NHィイD24ィエD2-N / NOィイD23ィエD2-N) medium until 12h, while at 48h the pH rose again to 6.0 and higher. This rise was accompanied by a decrease in the nitrate concentration at 24 and 48h. In contrast, the pH increased around the roots of chinese cabbage with time, while nitrate decreased, suggesting relationship between rhizosphere pH changes and preferential cation / anion uptake of plants. Also we observed the acidification of rhizosphere with iron and phosphorus deficiency, and by coexisting insoluble phosphate salts (AlPOィイD24ィエD2 and FePOィイD24ィエD2).In addition, we evaluated the root activity under the different fertilized conditions by image analysis of root color in stead of the judgment by eyes.The method we established can be applied to the nutritional diagnosis of plants.