Role of Cardiovascular-related Peptides in the Brain : Genetic, Molecular Biological, Morphological and Physiological Aspects.

心血管相关肽在大脑中的作用：遗传、分子生物学、形态学和生理学方面。

• 批准号: 10044332
• 负责人: YAMASHITA Hiroshi
• 金额: $ 2.24万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044332/
• 关键词: Vasopressin; Adrenomedullin; Oxytocin; Orexin; Leptin; Urocortin; PACAP; アドレノメデユリン; 循環; ペプチド; 視床下部; 室傍核; 視索上核; イオンチャネル; 学習

The purpose of the present study was to investigate the role of cardiovascular-related peptides in the central nervous system (CNS), using genetic, molecular biological, morphological and physiological approaches. These approaches focused on the integrative investigation of the role from the genetics to behavior of cardiovascular-related peptides in the CNS. The examined cardiovascular-related peptides were vasopressin, adrenomedullin, orexin/hypocretin, leptin, TRH, CRH, urocortin and PACAP. (1) The expression of the vasopressin gene in the hypothalamus was upregulated in genetically polydipsic mice. (2) Adrenomedullin-like immunoreactive cells were distributed in the hypothalamic area, including the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of rats. Central administration of adrenomedullin selectively activated oxytocin-secreting neurons in the PVN and the SON with elevation of plasma oxytocin. (3) Orexin/hypocretin was newly discovered orexigenic peptides. Orexin-pr … More oducing neurons were localized in the lateral hypothalamic area. Their axons project to various regions in the CNS. Central administration of orexin selectively activated hypothalamo-pituitary adrenal axis in rats. Neonatal development of orexin in rats were examined. The expression of the orexin gene was markedly increased 15 and 20 days after birth. (4) The food deprivation induced down-regulation of neuronal nitric oxide synthase gene, TRH gene and CRH gene in the PVN were prevented by replacement with leptin. (5) The immunoreactivity of urocotin-producing cells and fibers in the SON was increased in the SON of hypertensive salt sensitive Dahl rats. (6) Central administration of PACAP activated vasopressin-secreting cells in the PVN and the SON and caused an increase in vasopressin heteronuclear RNA in the PVN and the SON. Kainic acid-induced seizure caused a marked induction of the PACAP gene in the parvocellular division of the PVN of rats. Furthermore, it is important to examine the role of cardiovascular-related peptides in the CNS. Less

本研究的目的是探讨心血管相关肽在中枢神经系统（CNS）中的作用，采用遗传学，分子生物学，形态学和生理学的方法。这些方法集中于从遗传学到行为学的心血管相关肽在中枢神经系统中的作用的综合研究。检测的心血管相关肽有加压素、肾上腺髓质素、食欲素/下丘脑泌素、瘦素、TRH、CRH、尿皮质素和PACAP。(1)遗传性多饮小鼠下丘脑加压素基因表达上调。(2)肾上腺髓质素样免疫反应细胞分布于大鼠下丘脑室旁核（PVN）和视上核（SON）。肾上腺髓质素的中枢给药选择性激活PVN和SON中的催产素分泌神经元，血浆催产素升高。(3)食欲素/下丘脑泌素是新发现的促食欲肽。增食欲素 ...更多信息 产生神经元位于下丘脑外侧区。它们的轴突投射到CNS的各个区域。中枢给药增食欲素选择性激活大鼠下丘脑-垂体-肾上腺轴。对大鼠食欲素的新生发育进行了研究。食欲素基因的表达显着增加出生后15和20天。(4)用瘦素替代可防止食物剥夺引起的下丘脑室旁核神经元型一氧化氮合酶基因、TRH基因和CRH基因的下调。(5)高血压盐敏感Dahl大鼠SON内尿可肽产生细胞和纤维的免疫反应性增加。(6)PACAP的中枢给药激活了PVN和SON中的加压素分泌细胞，并引起PVN和SON中加压素异源RNA的增加。海人酸诱导的癫痫发作引起大鼠PVN小细胞分裂中PACAP基因的显著诱导。此外，研究心血管相关肽在CNS中的作用也很重要。少

项目成果

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