Imaging and Neural Circuits Core

摘要： 阿片类药物滥用是美国的一种主要流行病。仅在2017年，滥用处方和非法阿片类药物 导致超过6万人死亡。从阿片类止痛药的治疗性使用向破坏性的转变 物质滥用的发生，部分是由于中皮质边缘脑回路的不适应激活。这些是如何 不同的感觉、奖赏和压力神经回路在系统中起作用，细胞和分子水平保持不变 人们对此知之甚少。随着我们努力增进对阿片类药物使用障碍发展的理解，我们 需要发展对阿片类药物回路如何在奖赏和厌恶中发挥作用的基本见解。此外，我们 需要了解阿片类药物动物模型中这些回路是如何随着时间的推移而调制、改变和适应的 上瘾。我们的目标是利用威斯康星州大学多样化的调查团队来回答这些问题。我们 建议建立一个先进的成像和成瘾神经电路核心(INCC)来提供UW成瘾 研究人员、神经科学家和更广泛的社区拥有一套先进的神经技术，用于体内 神经电路的摄动与分析。除了构建并不断更新最先进的图像 作为一个设施，核心的主要作用将是培训NIDA P30调查员使用它，以加强 研究他们的NIDA资助的项目。我们还将向更大的研究社区传播这些工具 通过提供现场培训。这些资源将使密歇根大学和其他地方的研究人员能够确定 并纠正在阿片成瘾模型中观察到的神经回路适应不良。

Summary: Opioid abuse is a major epidemic in the United States. In 2017 alone, abuse of prescription and illicit opioids resulted in over 60,000 deaths. The transition from therapeutic use of opioid pain relievers to destructive substance abuse occurs, in part, through maladaptive activation of mesocorticolimbic brain circuits. How these various sensory, reward, and stress neural circuits act at the systems, cellular and molecular levels remain poorly understood. As we work to advance our understanding of the development of opioid use disorder, we need to develop fundamental insights into how opioid circuits function in reward and aversion. In addition, we need to learn how these circuits are modulated, altered, and adapted over time in animal models of opioid addiction. We aim to leverage the diverse team of investigators at UW towards answering these questions. We propose to establish an advanced Imaging and Addiction Neural Circuits Core (INCC) to provide UW addiction researchers, neuroscientists, and the wider community with a suite of advanced neurotechnologies, for in vivo neural circuit perturbation and analysis. Beyond building and continually renewing a state-of-the-art imaging facility, a principal role of the Core will be to train the NIDA P30 Investigators in its uses in order to enhance the research of their NIDA funded projects. We will also disseminate these tools to the larger research community by providing on-site training. Collectivity, these resources will enable researchers at UW and beyond to identify and correct neurocircuit maladaptation observed in opioid addiction models.