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University of Washington Center of Excellence in Opioid Addiction Research

华盛顿大学阿片类药物成瘾研究卓越中心

基本信息

批准号：
10152567
负责人：
Charles Chavkin
金额：
$ 132.39万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10152567
关键词：
Address Affect Animals Anxiety Disorders Award Behavior Behavioral Brain Brain imaging CRISPR/Cas technology Calcium Signaling Canis familiaris Cells Collaborations Communities Complex Computer Analysis Confocal Microscopy Coupled DSM-V Development Disease Drug Addiction Drug usage Education and Outreach Educational workshop Endoscopy Ensure Fiber Fostering Funding Gene Expression Genetic Genetic Techniques Goals Image Imaging Device Infrastructure Institution Investigation Laboratories Light Link Mental Depression Methodology Modernization Molecular Molecular Genetics Monitor Morphine Mus National Institute of Drug Abuse Neurobiology Neurons Neurosciences Opiate Addiction Opioid Pain Participant Photometry Photons Pilot Projects Postdoctoral Fellow Rattus Reagent Receptor Signaling Recording of previous events Reproducibility Research Research Personnel Research Training Resolution Resource Sharing Resources Risk Rodent Rodent Control Scientist Self Administration Substance Use Disorder Techniques Training Training and Education United States National Institutes of Health Universities Viral Viral Genes Visit Visualization Washington addiction archive data archived data awake base comorbidity computational neuroscience computerized tools data analysis pipeline design design and construction drug abstinence dysphoria genetic manipulation genetic resource graduate student imaging approach imaging genetics in vivo innovation insight meetings motivated behavior neural circuit neuroimaging novel novel therapeutics open data opioid use opioid use disorder optogenetics outreach ranpirnase recruit relapse risk research study resource guides sensor tool two photon microscopy two-photon undergraduate student vector web site

项目摘要

Overall Summary The “University of Washington Center of Excellence in Opioid Addiction Research” is designed to provide shared resources that would enhance efficiency and facilitate collaborative research for the study of the effects of opioids on neural circuits with the goal of understanding opioid addiction mechanisms and developing novel treatments for drug addiction. Center participants from 16 UW laboratories are using optogenetic control of rodent behavior, in vivo neuroimaging of single cell calcium signals and other receptor signaling probes, viral gene expression and CRISPR/cas9 manipulations to deconstruct and study opioid self-administration. This group has a long-history of highly productive, collaborative, cutting-edge research and training that will be strengthened by the shared resources provided by this award. The proposed Center would be comprised of four components: The Administrative Core will coordinate resource utilization, organize monthly Research Progress Meetings, organize training and outreach efforts. The Imaging and Neural Circuits Core would develop shared resources for in vivo brain imaging in rats and mice using 1-Photon endoscopy, Inscopix imaging, 2-Photon confocal microscopy, Spatial Light Modulation, fiber photometry and operant behaviors. The Molecular Genetics Resource Core would provide DIO-AAV / CRISPR-cas9 / Canine Adeno Viral reagents, develop new activity actuators and sensors and provide advanced training in cell-specific genetic manipulation coupled with behavioral and computational analysis. A Pilot Project Core would enable participants and trainees to initiate new projects utilizing the Imaging and Genetic Cores and would foster training and collaborations within the Center laboratory groups and university community at large. The Center would become a national neuroscience resource by providing training to visiting scientists, reagents for genetic manipulation and novel actuators/sensors on request from investigators at other institutions. It will provide workshops and summer courses for training advanced undergraduate and graduate students in optogenetics, computational neuroscience, and viral design & construction techniques. All of these components would be focused on understanding the changes in neural circuitry responsible for opioid addiction and on the development of new therapeutic tools based on these insights.

整体总结