Imaging and Neural Circuits Core

成像和神经电路核心

• 批准号: 10394248
• 负责人: Charles Chavkin
• 金额: $ 49.05万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10394248
• 关键词: Animal Model; Animals; Behavior; Behavior Control; Brain; Brain imaging; Calcium; Cells; Cessation of life; Code; Communities; Core Facility; Cues; Data Analyses; Data Science; Data Set; Detection; Development; Drug abuse; Educational workshop; Endoscopy; Epidemic; Exposure to; Funding; Goals; Image; Infrastructure; Laboratories; Learning; Light; Logistics; Methodology; Modeling; Molecular; National Institute of Drug Abuse; Neurons; Opiate Addiction; Opioid; Opioid Analgesics; Opsin; Optics; Pharmaceutical Preparations; Photometry; Pilot Projects; Positioning Attribute; Research; Research Personnel; Resolution; Resources; Rewards; Role; Sensory; Site; Stimulus; Stress; Substance abuse problem; System; Techniques; Technology; Therapeutic Uses; Time; Training; United States; United States National Institutes of Health; Universities; Washington; Work; addiction; awake; base; cell type; computational neuroscience; computerized tools; data analysis pipeline; illicit opioid; imaging approach; imaging facilities; in vivo; in vivo calcium imaging; in vivo imaging; innovative technologies; insight; meetings; neural circuit; neurotechnology; novel strategies; open data; opioid abuse; opioid use disorder; optogenetics; prescription opioid abuse; ranpirnase; relating to nervous system; remote control; sensor; spatiotemporal; tool; two photon microscopy; two-photon; voltage; wireless

Summary: Opioid abuse is a major epidemic in the United States. In 2017 alone, abuse of prescription and illicit opioids resulted in over 60,000 deaths. The transition from therapeutic use of opioid pain relievers to destructive substance abuse occurs, in part, through maladaptive activation of mesocorticolimbic brain circuits. How these various sensory, reward, and stress neural circuits act at the systems, cellular and molecular levels remain poorly understood. As we work to advance our understanding of the development of opioid use disorder, we need to develop fundamental insights into how opioid circuits function in reward and aversion. In addition, we need to learn how these circuits are modulated, altered, and adapted over time in animal models of opioid addiction. We aim to leverage the diverse team of investigators at UW towards answering these questions. We propose to establish an advanced Imaging and Addiction Neural Circuits Core (INCC) to provide UW addiction researchers, neuroscientists, and the wider community with a suite of advanced neurotechnologies, for in vivo neural circuit perturbation and analysis. Beyond building and continually renewing a state-of-the-art imaging facility, a principal role of the Core will be to train the NIDA P30 Investigators in its uses in order to enhance the research of their NIDA funded projects. We will also disseminate these tools to the larger research community by providing on-site training. Collectivity, these resources will enable researchers at UW and beyond to identify and correct neurocircuit maladaptation observed in opioid addiction models.

概括： 阿片类药物滥用是美国的一个主要流行病。仅 2017 年，滥用处方药和非法阿片类药物的情况 导致6万多人死亡。阿片类止痛药从治疗性用途转变为破坏性用途 药物滥用部分是由于中皮质边缘大脑回路的适应不良激活而发生的。这些如何 各种感觉、奖励和压力神经回路作用于系统，细胞和分子水平仍然存在 不太了解。当我们努力增进对阿片类药物使用障碍的发展的了解时，我们 需要对阿片类药物回路如何在奖赏和厌恶中发挥作用产生基本的见解。此外，我们 需要了解这些回路如何在阿片类药物动物模型中随着时间的推移进行调节、改变和适应 瘾。我们的目标是利用华盛顿大学多元化的研究团队来回答这些问题。我们 提议建立先进的成像和成瘾神经回路核心（INCC）以提供华盛顿大学成瘾服务 研究人员、神经科学家和更广泛的社区拥有一套先进的神经技术，用于体内 神经回路扰动和分析。超越构建和不断更新最先进的成像 设施，核心的主要作用是对 NIDA P30 调查员进行使用培训，以提高 NIDA 资助项目的研究。我们还将向更大的研究界传播这些工具 通过提供现场培训。总的来说，这些资源将使威斯康星大学及其他地区的研究人员能够确定 并纠正阿片类药物成瘾模型中观察到的神经回路适应不良。