University of Washington Center of Excellence in Opioid Addiction Research

总体汇总 “华盛顿大学阿片类药物成瘾研究卓越中心”旨在提供 共享资源，提高效率，促进合作研究，研究影响 阿片类药物对神经回路的作用，目的是了解阿片类药物成瘾机制， 戒毒治疗来自16个UW实验室的中心参与者正在使用光遗传学控制， 啮齿动物行为，单细胞钙信号和其他受体信号探针的体内神经成像，病毒 基因表达和CRISPR/cas9操作来解构和研究阿片类药物自我给药。这 集团具有悠久的历史，高生产力，协作，尖端的研究和培训，将是 通过这个奖项提供的共享资源得到加强。拟议的中心将由以下人员组成： 四个组成部分：行政核心将协调资源利用，组织每月研究， 进度会议，组织培训和外联工作。成像和神经回路核心将 开发共享资源，用于使用1-光子内窥镜在大鼠和小鼠中进行体内脑成像，Insopix 成像，双光子共聚焦显微镜，空间光调制，光纤光度学和操作行为。的 分子遗传学资源中心将提供DIO-AAV / CRISPR-cas9 /犬腺病毒试剂， 开发新的活动致动器和传感器，并提供细胞特异性遗传操作的高级培训 再加上行为和计算分析。核心试点项目将使参与者和 培训人员利用成像和遗传核心启动新项目，并将促进培训， 中心实验室团体和整个大学社区内的合作。该中心将 通过为来访的科学家提供培训，成为国家神经科学资源，遗传学试剂， 应其他机构研究人员的要求，提供了新型操纵和新型致动器/传感器。它将提供 举办讲习班和暑期课程，培训光遗传学高级本科生和研究生， 计算神经科学和病毒设计与构建技术。所有这些组件都将 专注于了解负责阿片类药物成瘾的神经回路的变化， 基于这些见解开发新的治疗工具。

Overall Summary The “University of Washington Center of Excellence in Opioid Addiction Research” is designed to provide shared resources that would enhance efficiency and facilitate collaborative research for the study of the effects of opioids on neural circuits with the goal of understanding opioid addiction mechanisms and developing novel treatments for drug addiction. Center participants from 16 UW laboratories are using optogenetic control of rodent behavior, in vivo neuroimaging of single cell calcium signals and other receptor signaling probes, viral gene expression and CRISPR/cas9 manipulations to deconstruct and study opioid self-administration. This group has a long-history of highly productive, collaborative, cutting-edge research and training that will be strengthened by the shared resources provided by this award. The proposed Center would be comprised of four components: The Administrative Core will coordinate resource utilization, organize monthly Research Progress Meetings, organize training and outreach efforts. The Imaging and Neural Circuits Core would develop shared resources for in vivo brain imaging in rats and mice using 1-Photon endoscopy, Inscopix imaging, 2-Photon confocal microscopy, Spatial Light Modulation, fiber photometry and operant behaviors. The Molecular Genetics Resource Core would provide DIO-AAV / CRISPR-cas9 / Canine Adeno Viral reagents, develop new activity actuators and sensors and provide advanced training in cell-specific genetic manipulation coupled with behavioral and computational analysis. A Pilot Project Core would enable participants and trainees to initiate new projects utilizing the Imaging and Genetic Cores and would foster training and collaborations within the Center laboratory groups and university community at large. The Center would become a national neuroscience resource by providing training to visiting scientists, reagents for genetic manipulation and novel actuators/sensors on request from investigators at other institutions. It will provide workshops and summer courses for training advanced undergraduate and graduate students in optogenetics, computational neuroscience, and viral design & construction techniques. All of these components would be focused on understanding the changes in neural circuitry responsible for opioid addiction and on the development of new therapeutic tools based on these insights.