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Structured chromatic light sheet microscopy

结构色光片显微镜

基本信息

批准号：
10171843
负责人：
Rongguang Liang
金额：
$ 18.2万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10171843
关键词：
3-Dimensional Address Biopsy Body part Cancer Detection Carcinoma Cell Nucleus Clinical Custom Detection Developing Countries Diagnosis Disease Disease Progression Early Diagnosis Effectiveness Environment Epithelial Equine mule Family suidae Frequencies Goals Image Imaging Device Imaging Techniques Knowledge Label Lateral Length Light Lighting Localized Disease Malignant Neoplasms Measures Methods Microscope Microscopy Neoplasm Metastasis Optical Coherence Tomography Optics Oral cavity Oral mucous membrane structure Organ Outcome Performance Phase Physiologic pulse Property Quality of life Resolution Resources Scanning Screening for Skin Cancer Site Skin Skin Cancer Source Speed Structure Surface Surgical margins Survival Rate System Thick Tissue Sample Tissue imaging Tissues Width cancer diagnosis clinical application contrast imaging cost design handheld equipment high resolution imaging imaging system improved innovation lens light scattering low and middle-income countries malignant mouth neoplasm microscopic imaging mortality novel novel imaging technique optical imaging point of care prototype reflectance confocal microscopy tool

项目摘要

Abstract The long-term goal of this project is to develop a compact, label-free, low-cost optical device for imaging epithelial tissues in cancer detection and diagnosis. 80-90% cancers arise as carcinomas in epithelial tissues. Various high resolution optical imaging techniques and devices have been developed for early detection and diagnosis of epithelial diseases, but most of them are complicated and expensive, not suitable for point-of-care applications and low resource setting environment. We propose to develop a structured chromatic light sheet microscope. The key innovation is the use of a white µLED array to generate structured chromatic light sheet, eliminating the need of external bulky light source and scanner or spatial light modulator to obtain 3D tissue image. Another key innovation is the method of chromatic slit confocal detection using a linear variable filter to block the out-of-the-light-sheet light to improve image contrast and resolution. The image resolution and contrast will be further improved by taking and processing three images modulated with phase-shifted structured light. It is also expected that the image resolution and contrast in deeper layers can be improved by focusing and imaging a long wavelength light sheet deeper into the tissue. In addition, volumetric image can be obtained by projecting structured light sheets sequentially through the focal plane with 2D µLED array. In this project, we will first develop a structured chromatic light sheet microscope (Aim 1), and then evaluate the performance of the prototyped structured chromatic light sheet microscope in imaging epithelial tissue (Aim 2). With the knowledge gained in Aims 1 and 2, we will also develop and evaluate a handheld structured chromatic light sheet microscope for epithelial tissue imaging (Aim 3). The concepts of chromatic light sheet and chromatic slit confocal detection will have profound impact on light scattering imaging and potential applications in detection and diagnosis of epithelial cancers. This project will result in an effective clinical tool for early detection of skin and oral cancers, which will significantly reduce disease progression and mortality and improve quality of life. Further, this affordable tool will be very practical for low-resource settings to reduce the burden of skin and oral cancers in low- and middle- income countries (LMICs). In addition, the proposed microscope could enable improved localization of biopsy sites and delineation of surgical margins, which will reduce the need for repeat and random biopsies.

摘要