Characterizing SARS-CoV-2 infection of human taste cells in culture

培养物中人类味觉细胞的 SARS-CoV-2 感染特征

• 批准号: 10177462
• 负责人: Paul A. S Breslin
• 金额: $ 17.45万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-12-01 至 2020-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177462
• 关键词: 2019-nCoV; Administrative Supplement; Affect; African Green Monkey; COVID-19; Cell Proliferation; Cell Survival; Cell model; Cells; Coronavirus Infections; Disease; Epithelial Cells; Functional disorder; Human; Individual; Infection; Kinetics; Malignant neoplasm of lung; Measures; Metabolic; Modeling; Pathogenicity; Patients; Predisposition; Reporting; Research Personnel; Signal Pathway; Signal Transduction; Smell Perception; TMPRSS2 gene; Taste Perception; Virus; Virus Diseases; Zika Virus; coronavirus receptor; human coronavirus; interest; kidney cell; parent grant; receptor; sweet taste perception

Abstract Relatedness of Supplement Aim to Parent Grant In the parent grant, we use cultured human taste (HBO) cells, pioneered at Monell by Co-Investigator Hakan Ozdener, to probe the metabolic sweet taste signaling pathway. HBO cells provide a useful model for probing taste signaling in culture, but they have also been shown useful for investigating the pathophysiology of certain neurotrophic viral diseases (e.g. Zika virus; see Ozdener et al., 2020). Using HBO cells to accomplish the Supplement Aim will advance our understanding of the pathogenicity of SARS-CoV-2 and other viruses that adversely affect taste and olfaction. Although many studies have reported taste and olfactory loss in individuals with COVID-19 disease, the underlying mechanisms and cellular effects in taste cells are not well understood. Due to changes in taste function in patients with COVID-19, it will be of particular interest to the parent grant to know if the subset of sweet taste cells is susceptible to infection by SARS-CoV-2.

抽象的 补充目标与家长补助金的相关性 在母基金中，我们使用了培养的人类味觉 (HBO) 细胞，该细胞是由莫内尔联合研究员哈坎 (Hakan) 首创的 Ozdener，探索代谢甜味信号通路。 HBO 细胞为探测提供了有用的模型 文化中的味觉信号，但它们也被证明可用于研究某些特定的病理生理学 神经营养性病毒性疾病（例如寨卡病毒；参见 Ozdener 等人，2020）。使用 HBO 细胞来完成 补充目标将加深我们对 SARS-CoV-2 和其他病毒致病性的了解 对味觉和嗅觉产生不良影响。尽管许多研究报告了个体的味觉和嗅觉丧失 对于 COVID-19 疾病，味觉细胞的潜在机制和细胞效应尚不清楚。 由于 COVID-19 患者的味觉功能发生变化，家长会特别感兴趣 了解甜味细胞子集是否易受 SARS-CoV-2 感染。

项目成果

Salivary Amylase: Digestion and Metabolic Syndrome.

• DOI: 10.1007/s11892-016-0794-7
• 发表时间: 2016-10
• 期刊: Current diabetes reports
• 影响因子: 4.2
• 作者: Peyrot des Gachons C;Breslin PA
• 通讯作者: Breslin PA

Bitter Taste Receptors (T2Rs) are Sentinels that Coordinate Metabolic and Immunological Defense Responses.

• DOI: 10.1016/j.cophys.2021.01.006
• 发表时间: 2021-04
• 期刊: Current opinion in physiology
• 影响因子: 2.5
• 作者: Harmon CP;Deng D;Breslin PAS
• 通讯作者: Breslin PAS

Oral Signals of Short and Long Chain Fatty Acids: Parallel Taste Pathways to Identify Microbes and Triglycerides.

• DOI: 10.1016/j.cophys.2021.01.008
• 发表时间: 2021-04
• 期刊: Current opinion in physiology
• 影响因子: 2.5
• 作者: Hanselman EC;Amado NJ;Breslin PAS
• 通讯作者: Breslin PAS

Coronavirus infection in chemosensory cells.

• DOI: 10.1007/s13365-023-01117-3
• 发表时间: 2023-02
• 期刊: JOURNAL OF NEUROVIROLOGY
• 影响因子: 3.2
• 作者: Donadoni, Martina;Kaminski, Rafal;Liao, Shuren;Al Janabi, Suhair;Margolskee, Robert F. F.;Ozdener, Mehmet Hakan;Sariyer, Ilker K. K.
• 通讯作者: Sariyer, Ilker K. K.

Gli3 is a negative regulator of Tas1r3-expressing taste cells.

• DOI: 10.1371/journal.pgen.1007058
• 发表时间: 2018-03
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Qin Y;Sukumaran SK;Jyotaki M;Redding K;Jiang P;Margolskee RF
• 通讯作者: Margolskee RF