NMR Console and Prodigy CryoProbe

NMR 控制台和 Prodigy CryoProbe

• 批准号: 10177110
• 负责人: NEIL K GARG
• 金额: $ 60万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177110
• 关键词: Award; Core Facility; Detection; Doctor of Philosophy; Equipment; Failure; Funding; Grant; Laboratories; Molecular; NMR Spectroscopy; Performance; Research Personnel; Research Support; Resources; Risk; Sampling; Scientist; Signal Transduction; United States National Institutes of Health; digital; experience; instrumentation

Project Summary A group of NIH supported investigators requests funds to purchase a NMR cryoprobe and upgrade the console of a 600 MHz NMR spectrometer. This equipment will significantly increase the signal sensitivity of an existing shared spectrometer that is housed in the NMR Laboratory of the UCLA Molecular Instrumentation Center Core Facility and bring high sensitivity NMR detection of heteronuclei to the facility. Specifically, we request funds to purchase a broadband Bruker Prodigy CryoProbe that increases NMR signal sensitivity by a factor of 2-4 over the conventional NMR probe. Additionally, we request funds to replace the outdated Bruker Avance I NMR console that was installed in 2002, with an Avance Neo 600 High Performance Digital console, which is needed to support the new cryoprobe. The new instrumentation is not only expected to be considerably more sensitive, but also more reliable than the existing NMR console and probe, and it will help to relieve the oversaturation usage of other magnets in the facility. The new equipment will be managed by two experienced Ph.D.-level staff scientists. Also, if this grant is awarded, UCLA has committed funds to purchase a 60X sample changer to further optimize the resources in the facility, increase usage capacity, reduce the risk for accidental failure, and show our institutional commitment to excellence in research supported by state-of-the-art instrumentation.

项目摘要 一组NIH支持的研究人员要求资金购买NMR冷冻探针并升级控制台 600兆赫核磁共振光谱仪的图像该设备将大大提高现有的信号灵敏度 位于UCLA分子仪器中心核心NMR实验室的共享光谱仪 设备和带来高灵敏度的核磁共振检测异核的设施。具体而言，我们要求资金 购买宽带Bruker Prodigy CryoProbe，可将NMR信号灵敏度提高2-4倍， 传统的核磁共振探针。此外，我们还申请资金更换过时的布鲁克Avance I NMR 2002年安装的控制台，配有Avance Neo 600高性能数字控制台， 以支持新的冷冻探针。新的仪器不仅预计将更加灵敏， 而且比现有的NMR控制台和探头更可靠， 在设施中使用其他磁铁。新设备将由两名经验丰富的博士管理-雇用人员 科学家此外，如果获得这笔赠款，加州大学洛杉矶分校已承诺资金购买60倍样品转换器，以进一步 优化设施中的资源，提高使用能力，降低意外故障的风险，并显示 我们的机构承诺，以卓越的研究支持国家的最先进的仪器。

项目成果

Cascade Synthesis of Fluorinated Spiroheterocyclic Scaffolding for Peptidic Macrobicycles

肽大二环氟化螺杂环支架的级联合成

• DOI: 10.1021/jacs.3c03071
• 发表时间: 2023
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Mendoza, Angel;Bernardino, Salvador J.;Dweck, Morris J.;Valencia, Isabel;Evans, Declan;Tian, Haowen;Lee, William;Li, Yu;Houk, Kendall N.;Harran, Patrick G.
• 通讯作者: Harran, Patrick G.