Orbitrap Tribrid Mass Spectrometer for Wayne State Proteomics

项目摘要/摘要 这项提案的目标是将Thermo Fisher Science Orbitrap日食质谱仪 具有电子转移解离(ETD)能力和高场非对称波形离子迁移率 波谱(FAIMS-Pro)设计外加纳米LC到韦恩州立大学的蛋白质组学核心。 蛋白质组学核心支持广泛的用户基础，包括9个主要用户和16个次要用户，这些用户 为该提案做出了贡献，并拥有NIH资助的项目，这些项目将通过访问 Orbitrap月食。韦恩州立大学的蛋白质组学核心也支持卡马诺斯癌症研究所 P30资助的癌症中心以及NIEHS资助的治疗P30中心。这个Orbitrap月食 具有深入分析复杂蛋白质组和基于等压标签的多路传输的独特能力 由于40赫兹的扫描速度和在ON上启用的实时搜索的独特功能- 板载计算机。合并到Orbitrap Eclipse中的计算改进使其成为 基于蛋白质组同位素定量的特殊仪器和较新的方法，如 数据独立分析。与核心经验丰富的质谱学家相结合， Orbitrap月食将是华盛顿州立大学NIH资助的调查人员研究项目的主要资产 在密歇根东南部地区。随着调查员需求的发展，他们已经形成了更多的需求 用于更高分辨率、更高灵敏度和更高质量精度的蛋白质组质谱学。这个 Orbitrap Eclipse是为满足当今蛋白质组分析的需求而设计的。这乐器擅长深沉。 测序和定量蛋白质组学以及翻译后的准确特征 修改。目前堆芯中的质谱计缺乏快速占空比和先进的离子光学 这使得在日食上探测低丰度物种成为例行公事。明确本地化的能力 多肽的翻译后修饰(PTM)是任何蛋白质组MS系统中的关键特征，并且 由于提供了多种碎片模式，因此在这方面，Eclipse表现突出。韦恩州立大学 大学和亨利·福特健康系统都坚定地致力于这项提议，因为 总计68万美元的新支持，用于仪器管理、运营、 维护、信息学和使用。蛋白质组学核心人员的技术专长 由于物理和管理基础设施都已就位，并准备立即将此新的 美国国立卫生研究院资助的生物医学研究项目的质谱仪。该仪器将提供 推动NIH资助的数十个正在进行的项目的变革性技术，以及催化 韦恩州立大学研究人员的新研究方向。

Project Summary/Abstract The goal of this proposal is to bring a Thermo Fisher Scientific Orbitrap Eclipse mass spectrometer with Electron Transfer Dissociation (ETD) capability and a High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS-Pro) devise plus a nano-LC to the Proteomics Core at Wayne State University. The Proteomics Core supports an extensive user base including 9 Major Users and 16 Minor Users who have contributed to the proposal and who have NIH-funded projects that will be advanced by having access to the Orbitrap Eclipse. The Proteomics Core at Wayne State also supports the Karmanos Cancer Institute and P30 funded Cancer Center as well as the NIEHS funded CURES P30 Center. This Orbitrap Eclipse has unique capabilities for deep profiling of complex proteomes and isobaric tag based multiplexing due to the 40 Hz scan speed and the unique feature of Real Time Search that is enabled on the on- board computer. The computational advances incorporated into the Orbitrap Eclipse make it an exceptional instrument for proteomic isotopic based quantitation and for newer approaches such as Data Independent Analysis. Combined with the experienced mass spectrometrists in the Core, the Orbitrap Eclipse will be a major asset to research programs of NIH-funded investigators at WSU and in the SE Michigan region. As investigator needs have evolved they have developed an increased need for higher resolution, greater sensitivity and higher mass accuracy proteomic mass spectrometry. The Orbitrap Eclipse is designed to address todays needs in proteomic analysis. The instrument excels at deep sequencing and quantitative proteomics as well as accurate characterization of post translational modifications. The current mass spectrometers in the Core lack fast duty cycles and the advanced ion optics that make detection of low abundance species routine on the Eclipse. The ability to unambiguously localize post translational modifications (PTM) in peptides is a critical feature in any proteomic MS system and the Eclipse is outstanding in this due to the availability of multiple fragmentation modes. Wayne State University and the Henry Ford Health System are both strongly committed to this proposal as evidenced by a combined $680,000 in new support for the instruments management, operation, maintenance, informatics and usage. The technical expertise of Proteomics Core personnel as well as the physical and administrative infrastructures are all in place and ready to immediately put this new mass spectrometer to work on NIH-funded biomedical research projects. This instrument will provide transformative technologies for advancing dozens of NIH-funded, ongoing projects, as well as catalyzing new research directions for investigators at Wayne State University.