Orbitrap Tribrid Mass Spectrometer for Wayne State Proteomics

项目总结/摘要 该提案的目标是将Thermo Fisher Scientific Orbitrap Eclipse质谱仪 具有电子转移解离（ETD）能力和高场非对称波形离子迁移率 光谱（FAIMS-Pro）设计加上纳米LC的蛋白质组学核心在韦恩州立大学。 Proteomics Core支持广泛的用户群，包括9个主要用户和16个次要用户， 为该提案做出了贡献，并且有NIH资助的项目，这些项目将通过访问 轨道探测器月食韦恩州立大学的蛋白质组学核心也支持Karmanos癌症研究所 和P30资助的癌症中心以及NIEHS资助的治愈P30中心。这次轨道探测器日食 具有复杂蛋白质组的深度分析和基于同量异位素标签的多路复用的独特能力 由于40 Hz的扫描速度和独特的真实的时间搜索功能， 板计算机。Orbitrap Eclipse中包含的计算进步使其成为 用于蛋白质组同位素定量和新方法的特殊仪器， 数据独立分析。再加上内核里经验丰富的质谱师， Orbitrap Eclipse将成为NIH资助的WSU研究人员研究计划的主要资产， 在密歇根州东南部地区。随着研究者需求的发展， 用于更高分辨率、更高灵敏度和更高质量准确度的蛋白质组质谱。的 Orbitrap Eclipse旨在满足当今蛋白质组学分析的需求。该仪器擅长深度 测序和定量蛋白质组学以及翻译后的准确表征 修改.目前核心中的质谱仪缺乏快速的占空比和先进的离子光学 这使得在日蚀号上探测低丰度物种成为常规。明确本地化的能力 肽中的翻译后修饰（PTM）是任何蛋白质组MS系统中的关键特征， Eclipse在这方面非常出色，因为它提供了多种碎片模式。韦恩州立大学 大学和亨利福特卫生系统都坚定地致力于这一建议， 证明这一点的是， 维护、信息和使用。蛋白质组学核心人员的技术专长以及 由于物理和行政基础设施都已到位，并准备立即将这一新的 质谱仪工作在NIH资助的生物医学研究项目。该仪器将提供 变革性技术，用于推进数十个NIH资助的正在进行的项目，以及催化 为韦恩州立大学的研究人员提供了新的研究方向。

Project Summary/Abstract The goal of this proposal is to bring a Thermo Fisher Scientific Orbitrap Eclipse mass spectrometer with Electron Transfer Dissociation (ETD) capability and a High-Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS-Pro) devise plus a nano-LC to the Proteomics Core at Wayne State University. The Proteomics Core supports an extensive user base including 9 Major Users and 16 Minor Users who have contributed to the proposal and who have NIH-funded projects that will be advanced by having access to the Orbitrap Eclipse. The Proteomics Core at Wayne State also supports the Karmanos Cancer Institute and P30 funded Cancer Center as well as the NIEHS funded CURES P30 Center. This Orbitrap Eclipse has unique capabilities for deep profiling of complex proteomes and isobaric tag based multiplexing due to the 40 Hz scan speed and the unique feature of Real Time Search that is enabled on the on- board computer. The computational advances incorporated into the Orbitrap Eclipse make it an exceptional instrument for proteomic isotopic based quantitation and for newer approaches such as Data Independent Analysis. Combined with the experienced mass spectrometrists in the Core, the Orbitrap Eclipse will be a major asset to research programs of NIH-funded investigators at WSU and in the SE Michigan region. As investigator needs have evolved they have developed an increased need for higher resolution, greater sensitivity and higher mass accuracy proteomic mass spectrometry. The Orbitrap Eclipse is designed to address todays needs in proteomic analysis. The instrument excels at deep sequencing and quantitative proteomics as well as accurate characterization of post translational modifications. The current mass spectrometers in the Core lack fast duty cycles and the advanced ion optics that make detection of low abundance species routine on the Eclipse. The ability to unambiguously localize post translational modifications (PTM) in peptides is a critical feature in any proteomic MS system and the Eclipse is outstanding in this due to the availability of multiple fragmentation modes. Wayne State University and the Henry Ford Health System are both strongly committed to this proposal as evidenced by a combined $680,000 in new support for the instruments management, operation, maintenance, informatics and usage. The technical expertise of Proteomics Core personnel as well as the physical and administrative infrastructures are all in place and ready to immediately put this new mass spectrometer to work on NIH-funded biomedical research projects. This instrument will provide transformative technologies for advancing dozens of NIH-funded, ongoing projects, as well as catalyzing new research directions for investigators at Wayne State University.