CORE-- Protein Interactions and Proteomics Facilities

核心——蛋白质相互作用和蛋白质组学设施

• 批准号: 6750903
• 负责人: PAUL M STEMMER
• 金额: $ 18.65万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6750903
• 关键词: biological signal transduction; biomedical facility; environmental toxicology; fluorescence polarization; fluorescence resonance energy transfer; mass spectrometry; protein binding; protein protein interaction; proteomics; surface plasmon resonance

Description (provided by applicant): The mission of the Protein Interactions and Proteomics (PIP) Facility Core is to enhance research productivity by providing the equipment and expertise necessary for analysis of protein interactions and cellular protein composition. The two components of the PIP Core provide instruments and expertise for identification of proteins and analysis of protein interactions. These two services require different instrumentation, but they both rely on expertise in protein chemistry, separation and analysis. The protein interactions component of the PIP Core provides instrumentation and services for detection of protein binding by Fluorescence Polarization (FP), Surface Plasmon Resonance (SPR), and Fluorescence Resonance Energy Transfer (FRET). These PIP Core technologies, which are used to examine protein interactions, strengthen established research programs of Center investigators with research programs focused on cellular signaling pathways. Invariably, the function of signaling pathways is dependent on the coordinated and regulated interactions between many different proteins. The instruments in the PIP Core produce sensitive, accurate and real time measurements of protein binding events both in vitro and in intact cells. Thus, the protein interactions component of the PIP Core provides a window through which Center investigators can ask questions about protein-protein interactions and the effects of those interactions on signaling pathways and cellular function. The protein identification component of the PIP Core provides access to mass spectrometers for identification of proteins and protein covalent modifications, such as phosphorylation, as well as services for preparation of samples for and running of mass-spec analysis. The PIP Core technologies that are used in protein identification enhance research productivity by providing a clear and easily accessible mechanism for determining the amino acid sequence of unknown proteins that the investigator has either isolated or identified as an important component in a mixture of proteins. The technologies provided by the PIP Core can also be used by Center investigators to identify those proteins in complex mixtures of proteins from body fluids or cells that are candidate biomarkers. Archiving and analysis of data from protein identification technologies requires software systems to maintain the data and expertise in bioinformatics to analyze the data. The EHS Center at Wayne State University has established a facility core for Microarray Analysis and Bioinformatics, which will provide these services to Center investigators. Thus, the protein identification component of the PIP Core provides access to technology for protein identification, protein profiling and biomarker identification.

描述(申请人提供)：蛋白质相互作用和蛋白质组学(PIP)的使命 设施核心是通过提供分析蛋白质相互作用和细胞蛋白质组成所需的设备和专业知识来提高研究生产率。PIP核心的两个组成部分为鉴定蛋白质提供了仪器和专业知识 以及蛋白质相互作用的分析。这两项服务需要不同的仪器，但它们都依赖于蛋白质化学、分离和分析方面的专业知识。PIP核心的蛋白质相互作用组件为通过荧光偏振(FP)、表面等离子体共振(SPR)和荧光共振能量转移(FRET)检测蛋白质结合提供仪器和服务。这些用于检查蛋白质相互作用的PIP核心技术，加强了中心研究人员既定的研究计划，重点是细胞信号通路的研究计划。信号通路的功能总是依赖于许多不同蛋白质之间的协调和调节的相互作用。PIP Core中的仪器对体外和完整细胞中的蛋白质结合事件进行灵敏、准确和实时的测量。因此，PIP核心的蛋白质相互作用部分提供了一个窗口，中心的研究人员可以通过这个窗口询问有关蛋白质-蛋白质相互作用以及这些相互作用对信号通路和细胞功能的影响。PIP Core的蛋白质鉴定组件提供了用于鉴定蛋白质和蛋白质共价修饰(如磷酸化)的质谱仪，以及用于制备样品和运行质谱仪分析的服务。用于蛋白质鉴定的PIP核心技术通过提供一种清晰且易于访问的机制来确定未知蛋白质的氨基酸序列，从而提高了研究效率。研究人员已经分离出未知蛋白质或将其确定为蛋白质混合物中的重要成分。PIP Core提供的技术也可以被中心调查人员用来识别来自体液或细胞的蛋白质复杂混合物中的那些蛋白质，这些蛋白质是候选生物标记物。来自蛋白质鉴定技术的数据的存档和分析需要软件系统来 维护生物信息学方面的数据和专业知识，以分析数据。韦恩州立大学的EHS中心已经建立了一个用于微阵列分析和生物信息学的设施核心，该中心将为中心的调查人员提供这些服务。因此，PIP核心的蛋白质鉴定组件提供了蛋白质鉴定、蛋白质图谱和生物标记物鉴定技术。