A Proposal to expand analytical capabilities at Wayne State University with a 400

描述（由申请人提供）：本申请的目标是将具有纳米LC的Applied Biosystems 4000 QTrap混合串联质谱仪引入韦恩州立大学的蛋白质组学核心。该仪器具有使用三重四极杆能力发现翻译后修饰的独特能力，并在仪器的线性离子阱组件中积累已识别的离子，以获得最大灵敏度。综合功能使4000 QTrap成为发现和定量的卓越仪器。4000 QTrap和蛋白质组学核心内训练有素的操作员的可用性将是WSU NIH资助的研究人员研究计划的主要资产。该文书将解决目前尚未充分满足的两个关键需求。第一个是发现蛋白质翻译后修饰的新位点。第二个是从非常小的样品中定量蛋白质和修饰蛋白质。韦恩州立大学或底特律大都市地区目前唯一可用的可用于蛋白质组学分析的MS/MS仪器是LTQ-XL（带ETD），该仪器由试图添加4000 QTrap的同一蛋白质组学核心操作。LTQ被大量使用，并且不具有执行全光谱中性损失扫描的能力，并且不是基于质量标签的定量或多反应监测的首选仪器。WSU EHS中心内的蛋白质组学设施核心已被确立为韦恩州立大学蛋白质组学服务的唯一来源。大学坚定地致力于这一应用，证明了以下支持：1）长期计划的维护和Q陷阱4000的操作。2)为蛋白质组学核心设施翻新的实验室空间。3)为技术人员的工资和培训提供资金，以操作和安排仪器的使用。4000 QTrap混合串联质谱仪是一种最先进的系统，具有无与伦比的检测和定位蛋白质翻译后修饰的能力，并可对肽进行相对和绝对定量。在华盛顿州立大学，这种仪器将允许更有效地使用已经到位的仪器进行蛋白质组分离，并将为NIH资助的研究人员提供有效的现代蛋白质组分析。公共卫生相关性：我们的细胞、器官和系统受到蛋白质可逆变化的调节，如磷酸化、氧化和脂质或糖的附着。了解疾病过程和验证医学疗法需要我们发现和测量蛋白质及其变化。4000 QTrap可高效搜索蛋白质修饰，并可对蛋白质和修饰蛋白质进行灵敏定量。

DESCRIPTION (provided by applicant): The goal of this application is to bring an Applied Biosystems 4000 QTrap hybrid tandem mass spectrometer with nano-LC to the Proteomics Core at Wayne State University. This instrument has unique capabilities for discovery of post translational modifications using the triple quadrupole capabilities and to accumulate identified ions in the linear ion trap component of the instrument for maximal sensitivity. The combined capabilities make the 4000 QTrap an exceptional instrument for both discovery and quantitation. The availability of the 4000 QTrap and a trained operator within the Proteomics Core will be a major asset to research programs of NIH-funded investigators at WSU. The instrument will address two critical needs that are currently not adequately met. The first of these is for the discovery of novel sites of post translational modification in proteins. The second is quantitation of proteins and modified proteins from very small samples. The only currently available MS/MS instrument at Wayne State University or in the metropolitan Detroit area that can be used for proteomic analysis is the LTQ-XL with ETD that is operated by the same Proteomics Core seeking to add the 4000QTrap. The LTQ is heavily used and does not have the capabilities to perform full spectrum neutral loss scans and is not the instrument of choice for mass tag based quantitation or multiple reaction monitoring. The Proteomics Facility Core within the EHS Center at WSU has been established as the only source for proteomic services at Wayne State. The University is strongly committed to this application as evidenced by the following support: 1) A long term plan for maintenance and operation of the Q Trap 4000. 2) Renovated laboratory space for the Proteomics Core Facility. 3) Funding for salary and training of a technician to operate and schedule usage of the instrument. The 4000 QTrap hybrid tandem mass spectrometer is a state-of-the-art system with unparalleled ability to detect and localize post-translational modifications in proteins and to perform both relative and absolute quantitation of peptides. At WSU, this instrument will allow more productive use of instrumentation already in place for proteomic fractionation and will provide efficient access to modern proteomic analysis for NIH- funded investigators. PUBLIC HEALTH RELEVANCE: Our cells, organs and systems are regulated by reversible changes in proteins such as phosphorylation, oxidation and the attachment of lipids or sugars. Understanding disease processes and validating medical therapies requires us to discover and measure the proteins and the changes in proteins. The 4000 QTrap efficiently searches for protein modifications and allows sensitive quantitation of proteins and modified proteins.