Q TRAP 4000 LC/MS/MS

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：本提案的目标是为韦恩州立大学提供一台带有2D nan-LC的LTQ-11000线性离子阱质谱仪。这种仪器，旨在进行肽序列分析，并确定和定位蛋白质的翻译后修饰，将是一个主要的资产，以研究计划的NIH资助的研究人员在WSU的研究主要集中在细胞信号转导通路的调节在健康和疾病状态。已经确定的是，这些途径的控制依赖于蛋白质相互作用和酶活性的调节变化，其中许多是通过翻译后修饰来调节的。LTQ线性离子阱质谱仪的主要用户研究由磷酸化或氧化调节的信号通路。他们的研究需要识别蛋白质和分析这些蛋白质在特定刺激和/或特定疾病状态下的翻译后修饰。解决这些问题的唯一明确方法是通过质谱法。在韦恩州立大学或底特律大都市地区没有MS/MS仪器可用于蛋白质组学分析。华盛顿州立大学EHS中心内的蛋白质组学设施核心已被确立为韦恩州立大学这些蛋白质组学服务的第一来源。该大学坚定地致力于这一建议，证明了以下支持：1）资助LTQ线性离子阱MS的维护和操作的长期计划。2）蛋白质组学核心设施的专用实验室空间，将容纳该仪器。3)为技术人员的工资和培训提供资金，以操作和安排仪器的使用。LTQ-11000线性离子阱质谱仪是最先进的系统，具有无与伦比的速度和检测和定位蛋白质翻译后修饰的能力。在WSU，该仪器将允许更有效地使用已经到位的仪器进行蛋白质组分离，并将为NIH资助的研究人员提供有效的现代蛋白质组分析，这些研究人员现在受到缺乏当地蛋白质组支持的限制。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The goal of this proposal is to bring a LTQ-11000 Linear Ion Trap Mass Spectrometer with 2D nan-LC to Wayne State University. This instrument, designed to perform peptide sequence analysis and to identify and localize post-translational modifications in proteins, will be a major asset to research programs of NIH-funded investigators at WSU whose research is focused primarily on the regulation of cellular signal transduction pathways in healthy and diseased states. It is well established that control of these pathways is dependent on protein interactions and regulated changes in enzyme activities, many of which are modulated by post- translation modifications. Primary Users of the proposed LTQ Linear Ion Trap Mass Spectrometer work on signaling pathways that are modulated by phosphorylation or oxidation. Their research requires identification of proteins and analysis of the post-translational modifications of those proteins in response to specific stimuli and/or in specific disease states. The only unequivocal way to address these questions is through mass spectrometry. There is no MS/MS instrument at Wayne State or in the metropolitan Detroit area that can be used for proteomic analysis. The Proteomics Facility Core within the EHS Center at WSU has been established as the first source for these proteomic services at Wayne State. The University is strongly committed to this proposal as evidenced by the following support: 1) A funded long term plan for maintenance and operation of the LTQ Linear Ion Trap MS. 2) Dedicated laboratory space for a Proteomic Core Facility that will house the instrument. 3) Funding for salary and training of a technician to operate and schedule usage of the instrument. The LTQ-11000 Linear Ion Trap Mass Spectrometer is a state-of-the-art system with unparalleled speed and the ability to detect and localize post-translational modifications in proteins. At WSU, this instrument will allow more productive use of instrumentation already in place for proteomic fractionation and will provide efficient access to modern proteomic analysis for NIH-funded investigators who are now limited by the lack of local proteomic support.