Orbitrap Velos with ETD for Wayne State Proteomics

Orbitrap Velos 与 ETD 用于韦恩州立蛋白质组学

• 批准号: 8247312
• 负责人: PAUL M STEMMER
• 金额: $ 57.5万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247312
• 关键词: Address; Area; Commit; Contract Services; Core Facility; Daughter; Ensure; Fractionation; Funding; Goals; Hybrids; Ions; Michigan; Peptides; Post-Translational Protein Processing; Protein Fragment; Proteins; Proteomics; Research; Research Personnel; Resolution; Services; Source; System; Training; United States National Institutes of Health; Universities; base; cost; instrument; instrumentation; mass spectrometer; meetings; metropolitan; nano; operation; programs; protein aminoacid sequence

DESCRIPTION (provided by applicant): The goal of this proposal is to bring a Thermo Fisher Scientific Orbitrap hybrid FT mass spectrometer with ETD and nano-LC to the Proteomics Core at Wayne State University. This instrument, has unique capabilities for peptide sequencing and protein identification due to the high resolution and 1-2 ppm mass accuracy. The capabilities of the Orbitrap Velos make it an exceptional instrument fo proteomic profiling, spectral counting and isotopic based quantitation. The availability of the Orbitrap Velos and trained operators in the Proteomics Core will be a major asset to research programs of NIH-funded investigators at WSU and in the SE Michigan region. The instrument will address two critical needs that are currently not adequately met. The first of these is for unambiguous protein identification based on accurate mass of peptide precursor and daughter ions. The second is localization of post translational modifications (PTM) in large protein fragments that can maintain multiple PTMs in a single peptide. The only currently available MS/MS instruments at Wayne State University or in the metropolitan Detroit area that can be used for proteomic analysis is the LTQ-XL with ETD that is operated by the same Proteomics Core seeking to add the Orbitrap. The Proteomics Facility Core is the only source for proteomic services at Wayne State. The University is strongly committed to this proposal as evidenced by the following support: 1) A commitment to fund the general operations of the Core at $125,000 per year. 2) $214,000 in matching funds to purchase the instrument. 3) $75,000 from the SOM to defray some of the cost for a long term service contract to ensure continual operation of the Orbitrap. The Orbitrap with ETD is a state-of-the-art system with unparalleled ability to determine the accurate mass of peptides and protein fragments and to localize post-translational modifications in proteins. At WSU, this instrument will allow more productive use of instrumentation already in place for proteomic fractionation and will provide efficient access to modern proteomic analysis for NIH-funded investigators. PUBLIC HEALTH RELEVANCE: Understanding disease processes and validating medical therapies requires us to discover and measure the proteins and the changes in proteins in our cells. The Orbitrap Velos with ETD mass spectrometer system accurately identifies proteins and protein modifications in intact proteins, large protein fragments and peptides. Adding the Orbitrap to the WSU proteomics Core will provide essential analytical services to dozens of NIH funded researchers.

描述（由申请人提供）：本提案的目标是将带有ETD和纳米LC的Thermo Fisher Scientific Orbitrap混合FT质谱仪引入韦恩州立大学的蛋白质组学核心。该仪器具有高分辨率和1-2 ppm的质量准确度，因此具有独特的肽测序和蛋白质鉴定功能。Orbitrap Velos的功能使其成为蛋白质组学分析、光谱计数和同位素定量的特殊仪器。Orbitrap Velos和蛋白质组学核心中训练有素的操作员的可用性将是WSU和东南密歇根地区NIH资助的研究人员研究计划的主要资产。该文书将解决目前尚未充分满足的两个关键需求。第一个是基于肽前体和子离子的准确质量进行明确的蛋白质鉴定。第二个是翻译后修饰（PTM）在大的蛋白质片段，可以保持在一个单一的肽多个PTM的本地化。韦恩州立大学或底特律大都市地区目前唯一可用的可用于蛋白质组学分析的MS/MS仪器是LTQ-XL（带ETD），该仪器由寻求添加Orbitrap的同一蛋白质组学核心操作。蛋白质组学中心是韦恩州立大学蛋白质组学服务的唯一来源。该大学坚定地致力于这一建议，证明了以下支持：1）承诺资助核心的一般业务，每年125 000美元。 2)214,000美元的配套资金来购买仪器。 3)从SOM中拨款75，000美元，用于支付长期服务合同的部分费用，以确保轨道阱的持续运营。带有ETD的Orbitrap是一种最先进的系统，具有无与伦比的能力来确定肽和蛋白质片段的准确质量，并定位蛋白质中的翻译后修饰。在WSU，该仪器将允许更有效地使用已经到位的仪器进行蛋白质组分离，并将为NIH资助的研究人员提供有效的现代蛋白质组分析。 公共卫生关系：了解疾病过程和验证医学疗法需要我们发现和测量我们细胞中的蛋白质及其变化。Orbitrap Velos with ETD质谱仪系统可准确识别完整蛋白、大蛋白片段和肽中的蛋白质和蛋白质修饰。将Orbitrap添加到WSU蛋白质组学核心将为数十名NIH资助的研究人员提供必要的分析服务。