Orbitrap Velos with ETD for Wayne State Proteomics

描述（由申请人提供）：本提案的目标是将Thermo Fisher Scientific Orbitrap混合FT质谱计与ETD和纳米lc带到韦恩州立大学的蛋白质组学核心。由于高分辨率和1- 2ppm的质量精度，该仪器具有独特的肽测序和蛋白质鉴定能力。Orbitrap Velos的功能使其成为蛋白质组学分析，光谱计数和基于同位素定量的特殊仪器。Orbitrap Velos的可用性和训练有素的蛋白质组学核心操作人员将成为美国国立卫生研究院资助的华盛顿州立大学和密歇根东南部地区研究人员研究项目的主要资产。该文书将解决目前未得到充分满足的两个关键需求。其中第一个是基于肽前体和子离子的准确质量进行明确的蛋白质鉴定。第二种是翻译后修饰（PTM）在大蛋白片段中的定位，可以在单个肽中维持多个PTM。目前唯一可用的MS/MS仪器在韦恩州立大学或大都会底特律地区，可以用于蛋白质组学分析是LTQ-XL与ETD，是由同一个Proteomics Core寻求添加Orbitrap操作。蛋白质组学设施核心是韦恩州立大学蛋白质组学服务的唯一来源。联合国大学坚决支持这项建议，其证据是下列支持：1)承诺每年为核心的一般业务提供$125 000的经费。2) 214,000美元的配套资金用于购买该仪器。3)从SOM获得75,000美元，用于支付长期服务合同的部分费用，以确保Orbitrap的持续运行。带ETD的Orbitrap是一种最先进的系统，具有无与伦比的能力，可以确定肽和蛋白质片段的准确质量，并定位蛋白质的翻译后修饰。在华盛顿州立大学，该仪器将允许更有效地使用现有的仪器进行蛋白质组学分离，并将为美国国立卫生研究院资助的研究人员提供有效的现代蛋白质组学分析。

DESCRIPTION (provided by applicant): The goal of this proposal is to bring a Thermo Fisher Scientific Orbitrap hybrid FT mass spectrometer with ETD and nano-LC to the Proteomics Core at Wayne State University. This instrument, has unique capabilities for peptide sequencing and protein identification due to the high resolution and 1-2 ppm mass accuracy. The capabilities of the Orbitrap Velos make it an exceptional instrument fo proteomic profiling, spectral counting and isotopic based quantitation. The availability of the Orbitrap Velos and trained operators in the Proteomics Core will be a major asset to research programs of NIH-funded investigators at WSU and in the SE Michigan region. The instrument will address two critical needs that are currently not adequately met. The first of these is for unambiguous protein identification based on accurate mass of peptide precursor and daughter ions. The second is localization of post translational modifications (PTM) in large protein fragments that can maintain multiple PTMs in a single peptide. The only currently available MS/MS instruments at Wayne State University or in the metropolitan Detroit area that can be used for proteomic analysis is the LTQ-XL with ETD that is operated by the same Proteomics Core seeking to add the Orbitrap. The Proteomics Facility Core is the only source for proteomic services at Wayne State. The University is strongly committed to this proposal as evidenced by the following support: 1) A commitment to fund the general operations of the Core at $125,000 per year. 2) $214,000 in matching funds to purchase the instrument. 3) $75,000 from the SOM to defray some of the cost for a long term service contract to ensure continual operation of the Orbitrap. The Orbitrap with ETD is a state-of-the-art system with unparalleled ability to determine the accurate mass of peptides and protein fragments and to localize post-translational modifications in proteins. At WSU, this instrument will allow more productive use of instrumentation already in place for proteomic fractionation and will provide efficient access to modern proteomic analysis for NIH-funded investigators. PUBLIC HEALTH RELEVANCE: Understanding disease processes and validating medical therapies requires us to discover and measure the proteins and the changes in proteins in our cells. The Orbitrap Velos with ETD mass spectrometer system accurately identifies proteins and protein modifications in intact proteins, large protein fragments and peptides. Adding the Orbitrap to the WSU proteomics Core will provide essential analytical services to dozens of NIH funded researchers.