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Molecular Phenotyping Core

分子表型核心

基本信息

批准号：
10190914
负责人：
Subramaniam Pennathur
金额：
$ 12.13万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10190914
关键词：
Acyl Coenzyme A Address Adipocytes Amino Acids Animals Area Basic Science Bioinformatics Biological Biological Assay Carnitine Cells Ceramides Clinical Clinical Research Collaborations Complement Complex Consultations Consumption Cost Measures Coupled Cultured Cells Data Data Analyses Data Analytics Data Set Development Disease Electrospray Ionization Equipment Exercise Fatty Acids Funding Gas Chromatography Gene Expression Genus Hippocampus Glycolysis High Pressure Liquid Chromatography Human Resources In Vitro Infrastructure Label Laboratories Libraries Lipids Liquid Chromatography Mass Chromatography Measurement Measures Metabolic Metabolism Methods Michigan Mitochondria Modeling Molecular Molecular Analysis Mus Network-based Nucleic Acids Nutrition Disorders Nutritional Study Obesity Phenotype Phospholipids Physiological Plasma Postdoctoral Fellow Procedures Process Production Protocols documentation Protons Research Research Personnel Respiration Rodent Sampling Sensitivity and Specificity Services Shotguns Standardization Structure Surveys System Techniques Technology Testing Time Tissues Training Trans Fatty Acids Translational Research Urine Visualization Weight maintenance regimen analytical tool base bioinformatics tool biological systems biomarker identification cost effectiveness data integration data pipeline detection method graduate student high throughput screening human subject human tissue in vivo innovation insight instrument interest large datasets lipidomics metabolic abnormality assessment metabolomics molecular phenotype multiple omics novel nutrition obesity in children pre-clinical programs response small molecule stable isotope stem temporal measurement

项目摘要

Abstract/Summary - MPC The Molecular Phenotyping Core will provide analytical tools to the MNORC investigators to elucidate molecular mechanisms of disease relevant to obesity and nutritional disorders including structural identification and quantification of metabolites, perform functional metabolic studies and integrate multi-omic datasets to provide biological context. These include identifying unknown metabolites, developing new methods for targeted metabolomics in a range of biological matrices, validating new biological response indicators and providing biological context to diverse metabolomic measurements by providing bioinformatics analysis and developing new bioinformatics tools. In addition to providing instrumental infrastructure, the Core staff will provide consultation and collaboration to apply metabolomics and data analytical platforms in nutrition and obesity research. The Molecular Phenotyping Core will optimize the efficiency and cost-effectiveness by providing these services to MNORC investigators through a centralized laboratory. In the past five years the core has provided standardized analytical techniques for the analysis of small molecule metabolites such as amino acid, lipid and nucleic acid metabolites in samples from murine, rodent and human tissues, plasma, and urine, and cultured cells. New offerings have included novel untargeted metabolomics platforms, metabolic flux analysis and data interrogation and bioinformatics tools which will enhance the infrastructure available for the MNORC investigators. Emphasis will also be placed on fulfilling the needs of Investigators of the MNORC who will gain maximum benefit from the power of molecular analysis. We are particularly interested in addressing three areas of need: first, applying the unique sensitivity and specificity of molecular Phenotyping techniques to broaden understanding of nutritional disorders ; second, the development of innovative techniques for the detection and structural elucidation of nutrition-related biomolecules; third, provide data integration and bioinformatic tools relevant to metabolomics studies; and fourt, providing training for graduate students and postdoctoral fellows with an interest in nutrition research. By centralizing and standardizing procedures, the Core provides a common set of analytical tools that will lead to a unified understanding of molecular mechanisms involved in physiologic and pathophysiologic processes underlying obesity and other nutrition related disorders.

摘要/摘要- MPC