Molecular Phenotyping Core

分子表型核心

• 批准号: 8016405
• 负责人: Subramaniam Pennathur
• 金额: $ 25.85万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8016405
• 关键词: Acids; Address; Amines; Amino Acids; Area; Arts; Biometry; Clinical; Collaborations; Consultations; Cultured Cells; Detection; Development; Disease; Experimental Designs; Genus Hippocampus; Human; In Vitro; Individual; Informatics; Intervention; Isotopes; Laboratories; Lead; Lipids; Mass Spectrum Analysis; Metabolism; Michigan; Mitochondria; Molecular; Mus; Nucleic Acids; Nutritional Study; Obesity; Physiological; Plant Sources; Plasma; Population Study; Postdoctoral Fellow; Price; Procedures; Process; Research; Research Infrastructure; Research Personnel; Rodent; Sampling; Sensitivity and Specificity; Services; Subcellular Fractions; Systems Analysis; Techniques; Technology; Training; Urine; analytical method; analytical tool; cost; cost effectiveness; design; established cell line; graduate student; human tissue; innovation; instrumentation; interest; meetings; metabolic abnormality assessment; metabolomics; molecular phenotype; new technology; nutrition; research study; small molecule; tool

Molecular Phenotyping Core The Molecular Phenotyping Cere will provide analytical tools te the MNORC investigators to permit structural identification and quantification ef metabolites and perform functional metabolic studies. In addition to providing instrumental infrastructure, the Cere staff will provide consultation and collaboration te apply metabolomic platforms in nutrition and obesity research. Where needed, the Cere laboratories will assist in development of new analytical methods te meet the research objectives of the investigators. The Molecular Phenotyping Core will optimize the efficiency and cost-effectiveness by providing these services te MNORC investigators through a central laboratory. This avoids the need for individual investigators te purchase and maintain high price instrumentation in their own laboratories and avoids the high cost of commercial analytical services. In the past three years we have standardized and implemented several analytical techniques for the analysis of small molecule metabolites such as amine acid, lipid and nucleic acid metabolites in samples from murine, rodent and human tissues, plasma, and urine, and cultured cells. Emphasis will also be placed on fulfilling the needs ef Investigators efthe MNORC who will gain maximum benefit from the power of mass spectrometric analysis. We are particularly interested in addressing three areas ef need: first, applying the unique sensitivity and specificity ef mass spectrometry te detect and quantify lew abundance molecules of interest in nutrition research; second, the develepment ef innovative mass spectrometric techniques for the detection and structural elucidation of nutrition-related biomolecules; and three, providing MS training for graduate students and postdoctoral fellows with an interest in nutrition research. By centralizing and standardizing procedures, the Core provides a common set ef analytical tools that will lead to a unified understanding of molecular mechanisms involved in physiologic and pathophysiologic precesses underlying obesity and other nutrition related disorders.

分子表型核心 分子表型 Cere 将为 MNORC 研究人员提供分析工具，以便对代谢物进行结构鉴定和定量，并进行功能代谢研究。除了提供工具性基础设施外，Cere 工作人员还将提供咨询和协作，以将代谢组学平台应用于营养和肥胖研究。如果需要，Cere 实验室将协助开发新的分析方法，以满足研究人员的研究目标。分子表型核心将通过中心实验室为 MNORC 研究人员提供这些服务，从而优化效率和成本效益。这避免了个别研究人员在自己的实验室购买和维护高价仪器的需要，并避免了商业分析服务的高成本。在过去三年中，我们标准化并实施了多种分析技术，用于分析小鼠、啮齿动物和人体组织、血浆和尿液以及培养细胞样本中的氨基酸、脂质和核酸代谢物等小分子代谢物。重点还将放在满足 MNORC 调查人员的需求上，他们将从质谱分析的力量中获得最大的利益。我们对解决 ef 需求的三个领域特别感兴趣：首先，应用 ef 质谱法独特的灵敏度和特异性来检测和量化营养研究中感兴趣的低丰度分子；第二，开发用于营养相关生物分子检测和结构解析的创新质谱技术；第三，为对营养研究感兴趣的研究生和博士后提供硕士学位培训。通过集中和标准化程序，核心提供了一套通用的分析工具，这将导致对肥胖和其他营养相关疾病的生理和病理生理过程所涉及的分子机制的统一理解。