Molecular Phenotyping Core

分子表型核心 分子表型鉴定中心将为MNORC研究人员提供分析工具，以便对代谢物进行结构鉴定和量化，并进行功能代谢研究。除了提供仪器基础设施外，CERE的工作人员还将在营养和肥胖研究中提供应用代谢组学平台的咨询和合作。如有需要，CEE实验室将协助开发新的分析方法，以满足研究人员的研究目标。分子表型鉴定核心将通过一个中心实验室为MNORC研究人员提供这些服务，从而优化效率和成本效益。这就避免了个别研究人员在自己的实验室购买和维护高价仪器的需要，并避免了商业分析服务的高昂成本。在过去的三年里，我们标准化并实施了几种分析技术，用于分析小分子代谢物，如小鼠、啮齿动物和人体组织、血浆和尿液以及培养细胞中的氨基酸、脂肪和核酸代谢物。重点还将放在满足MNORC调查人员的需求上，他们将从质谱分析的能力中获得最大好处。我们特别感兴趣的是解决以下三个方面的需求：第一，应用独特的灵敏度和特异度，对营养研究中感兴趣的低丰度分子进行检测和量化；第二，开发创新的质谱学技术，用于检测和确定与营养相关的生物分子的结构；以及第三，为对营养研究感兴趣的研究生和博士后提供MS培训。通过集中和标准化程序，核心提供了一套通用的分析工具，将导致对肥胖和其他营养相关疾病的生理和病理生理过程中涉及的分子机制有一个统一的理解。

Molecular Phenotyping Core The Molecular Phenotyping Cere will provide analytical tools te the MNORC investigators to permit structural identification and quantification ef metabolites and perform functional metabolic studies. In addition to providing instrumental infrastructure, the Cere staff will provide consultation and collaboration te apply metabolomic platforms in nutrition and obesity research. Where needed, the Cere laboratories will assist in development of new analytical methods te meet the research objectives of the investigators. The Molecular Phenotyping Core will optimize the efficiency and cost-effectiveness by providing these services te MNORC investigators through a central laboratory. This avoids the need for individual investigators te purchase and maintain high price instrumentation in their own laboratories and avoids the high cost of commercial analytical services. In the past three years we have standardized and implemented several analytical techniques for the analysis of small molecule metabolites such as amine acid, lipid and nucleic acid metabolites in samples from murine, rodent and human tissues, plasma, and urine, and cultured cells. Emphasis will also be placed on fulfilling the needs ef Investigators efthe MNORC who will gain maximum benefit from the power of mass spectrometric analysis. We are particularly interested in addressing three areas ef need: first, applying the unique sensitivity and specificity ef mass spectrometry te detect and quantify lew abundance molecules of interest in nutrition research; second, the develepment ef innovative mass spectrometric techniques for the detection and structural elucidation of nutrition-related biomolecules; and three, providing MS training for graduate students and postdoctoral fellows with an interest in nutrition research. By centralizing and standardizing procedures, the Core provides a common set ef analytical tools that will lead to a unified understanding of molecular mechanisms involved in physiologic and pathophysiologic precesses underlying obesity and other nutrition related disorders.