Developmental Core

发展核心

• 批准号: 10222674
• 负责人: Kurunthachalam Kannan
• 金额: $ 34.06万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10222674
• 关键词: Adipose tissue; Amniotic Fluid; Biological; Biological Assay; Biological Monitoring; Blood; Chemicals; Chromium; Client; Coupling; Development; Environment; Follicular Fluid; Gas Chromatography; Generations; Goals; Hair; Health; Human; Human Milk; Human Resources; Inductively Coupled Plasma Mass Spectrometry; International; Laboratories; Liquid Chromatography; Liquid substance; Measurement; Measures; Meconium; Methods; Nail plate; Nickel; Organic Chemicals; Placenta; Publications; Quality Control; Rare Earth Metals; Research; Resources; Running; Sampling; Seminal Plasma; Spottings; Suggestion; Technology; Time; Tissues; Tooth structure; Trace Elements; Umbilical Cord Blood; Umbilical cord structure; Validation; Vanadium; analytical method; base; bone; cost; detection limit; environmental chemical; environmental stressor; experience; human tissue; improved; instrumentation; mass spectrometer; novel; novel marker; novel strategies; programs; response biomarker; tandem mass spectrometry; toxic metal

ABSTRACT – DEVELOPMENTAL CORE The overall goal of the Wadsworth Center’s Human Health Exposure Analysis Resource (WC-HHEAR) Developmental Core (Dev. Core) is to capitalize on the existing resources, expertise, and capacity at Wadsworth to develop and validate novel methods and analytical approaches for quantitative measurements of emerging chemicals in biospecimens, i.e., pushing the boundaries of human biomonitoring. Drs. Kannan and Parsons, the MPIs on this proposal, are leaders in their respective fields with more than 700 combined publications. They have spent the last two decades building the human biomonitoring program at Wadsworth – in Dr. Parsons’ Trace Elements Laboratory (TREL) and in Dr. Kannan’s Human Organics Biomonitoring Lab (HOBL). These efforts include assembling the current analytical capability; discovering emerging chemicals in human tissues and fluids; developing and validating new methods of analysis; and supporting the advancement of human health studies and the environment. Both HOBL and TREL have expansive lists of assays and capabilities that are part of the repertoire committed to support the WC-HHEAR targeted analysis resource (TAR). One of the Dev. Core’s functions will be to improve existing methods, requiring less sample volume for analysis, doing more with a limited amount of sample, improving detection limits, increasing sample throughput, reducing costs, and leveraging state-of-the-art technologies as they become available. For example, the proposed Dev. Core will investigate extractions that can be implemented for use on multiple classes of organic chemicals and improving detectability for trace elements using a new generation of technology based on inorganic tandem mass spectrometry. New capabilities will be developed in the Dev. Core and validated according to the international standards for single-laboratory method validation. The Dev. Core will build on its existing expertise with non-traditional matrices to offer more targets in more matrix types and will respond to HHEAR – driven by either discoveries in the untargeted resource, increased demand from HHEAR clients, or suggestions from the HHEAR program office. The transfer of these improvements to current methods will be facilitated by the WC-HHEAR administrative core, where key staff are integrated into both the TAR and Dev. Core. It is only through the progress outlined above that high-quality, quantitative, exposome research can flourish.

摘要- 沃兹沃斯中心的人类健康暴露分析资源（WC-HHEAR）的总体目标 开发核心（Dev.核心）是利用现有的资源、专业知识和能力， 沃兹沃斯开发和验证新的方法和分析方法的定量 测量生物样本中出现的化学物质，即，挑战人类的极限 生物监测Kannan博士和Parsons博士是该提案的MPI，他们是各自领域的领导者 出版物超过700种。在过去的二十年里， 生物监测计划在沃兹沃斯-在博士帕森斯的微量元素实验室（TREL）和博士。 Kannan的人体有机物生物监测实验室（HOBL）。这些努力包括组装电流 分析能力;发现人体组织和体液中新出现的化学物质;开发和 验证新的分析方法;支持人类健康研究的进步， 环境HOBL和TREL都有广泛的分析和功能列表，这些列表是 致力于支持WC-HHEAR靶向分析资源（TAR）的库。其中一个Dev。 核心的功能将是改善现有的方法，需要更少的样品量进行分析， 有限的样品量，提高检测限，增加样品吞吐量，减少 成本，并利用最先进的技术，因为它们变得可用。例如，拟议的 Dev.核心将调查提取，可以实施用于多种类别的有机 利用新一代技术， 无机串联质谱将在Dev中开发新功能。核心和验证 按照国际标准进行单实验室方法验证。Dev。核心将建立 利用其现有的非传统矩阵专业知识，以提供更多矩阵类型的更多目标，并将 响应HHEAR -由非目标资源的发现， HHEAR客户或HHEAR项目办公室的建议。将这些改进转移到 目前的方法将由WC-HHEAR行政核心提供便利，核心的主要工作人员将被整合 进入TAR和Dev。核心只有通过上述进展， 定量的、麻烦的研究可以蓬勃发展。