Targeted Analysis

项目总结/摘要-目标 沃兹沃斯中心人类健康目标分析资源（TAR）的总体目标 和暴露分析资源（WC-HHEAR）提供最先进的分析设施， HHEAR客户对环境暴露和环境污染物的影响进行研究 对人类健康的影响。拟议的TAR将利用该中心广泛的基础设施，专业知识， 作为一个世界级的生物监测实验室，已经建立了几十年的部门声誉 环境健康科学（DEHS）。在DEHS内，两个分析实验室小组进行有针对性的分析 环境污染物：微量元素实验室（TREL）和人体有机物生物监测实验室 （HOBL）。帕特里克帕森斯博士是DEHS的主任，负责监督TREL，而Kurunthlachalam Kannan博士 是负责监督HOBL的DEHS副主任。帕森斯博士和Kannan博士（本提案的MPI） 超过65年的分析化学应用于环境测量的综合经验 人体组织和体液中的污染物。HOBL分析了生物标本中30类微量有机物 污染物总计数百种分析物，包括但不限于多氯联苯、多环联苯、 芳香烃、全氟化合物、溴化阻燃剂、杀虫剂（例如， 有机磷、有机氯、拟除虫菊酯和烟碱类杀虫剂）、邻苯二甲酸酯（包括 代谢物、替代物和新兴化学品），药物（例如，阿片样物质），应激的生物标志物（例如，氧化 DNA、蛋白质和脂质的应激标志物），生活方式生物标志物（例如，烟草烟雾）和营养生物标志物 (e.g.,植物雌激素、微量营养素）。TREL小组分析了生物标本中多达22种微量元素， 包括有毒（铅、镉、汞和砷）和必需（铜、硒、锌）元素 血液、尿液和血清/血浆，加上包括镧系元素在内的16种稀土元素。两 HOBL和TREL拥有分析非传统基质的广泛专业知识，如胎盘、脐带血、婴儿 牙齿、干血斑、头发、指甲、羊水、母乳、唾液和其他组织。西藏自治区将利用 关于沃兹沃斯的国际声誉和领导地位，以促进实验室分析的协调， 生物监测和暴露分析基于严格的质量控制，这可以通过以下方面得到证明：1）组织 外部能力测试计划和教育实验室间研究（针对新兴检测）; 2）提供 通过传播标准操作规程，提供专题专门知识; 3）为以下人员提供实践培训： 来自其他实验室的工作人员;以及4）生产良好表征的质量控制和参考材料， 提供给更广泛的科学界。丰富和广泛的经验、知识、技能和 多年来，WC-HHEAR赢得了良好的声誉，包括成功参加CHEMICAL， TAR，将使HHEAR生物组学在各种曝光范围内成功顺利实施 这需要严格的分析质量控制。

PROJECT SUMMARY / ABSTRACT – TARGETED The overall objective of the Targeted Analysis Resource (TAR) within the Wadsworth Center’s Human Health and Exposure Analysis Resource (WC-HHEAR) is to provide access to state-of-the-art, analytical facilities to HHEAR clients conducting studies into environmental exposures and the effects of environmental contaminants on human health. The proposed TAR will leverage the Center’s extensive infrastructure, expertise, and reputation as a world-class biomonitoring laboratory that has been built over several decades in the Department of Environmental Health Sciences (DEHS). Within DEHS two analytical lab groups conduct targeted analyses for environmental contaminants: the Trace Elements Lab (TREL) and the Human Organics Biomonitoring Lab (HOBL). Dr. Patrick Parsons is the Director of DEHS overseeing the TREL, while Dr. Kurunthlachalam Kannan is Deputy Director of DEHS overseeing the HOBL. Drs. Parsons and Kannan (the MPIs of this proposal) have more than 65 years of combined experience in analytical chemistry applied to measuring environmental contaminants in human tissues and fluids. The HOBL analyzes biospecimens for 30 classes of trace organic contaminants totaling hundreds of analytes including, but not limited to, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, perfluorinated compounds, brominated flame retardants, pesticides (e.g., organophosphate, organochlorine, synthetic pyrethroid and neonicotinoid insecticides), phthalates (including metabolites, replacements and emerging chemicals), drugs (e.g., opioids), biomarkers of stress (e.g., oxidative stress markers of DNA, protein and lipids), life style biomarkers (e.g., tobacco smoke) and nutritional biomarkers (e.g., phytoestrogens, micronutrients). The TREL group analyzes biospecimens for up to 22 trace elements that including toxic (lead, cadmium, mercury and arsenic) and essential (copper, selenium, zinc) elements in whole blood, urine, and serum/plasma, plus the 16 rare earth elements, which includes the lanthanides series. Both HOBL and TREL have extensive expertise analyzing non-traditional matrices such as placenta, cord blood, baby teeth, dried blood spots, hair, nails, amniotic fluid, breast milk, saliva, and other tissues. The TAR will capitalize on Wadsworth’s international reputation and leadership to promote harmonization of laboratory analyses for biomonitoring and exposure analysis based on rigorous quality control which is evidenced by 1) organizing external proficiency testing programs and educational interlaboratory studies (for emerging assays); 2) providing subject matter expertise through disseminating standard operating protocols; 3) offering hands-on training for staff from other laboratories; and 4) producing well-characterized quality control and reference materials that are available to the wider scientific community. The wealth and breadth of experience, knowledge, skills and reputation that WC-HHEAR has garnered over the years, including its successful participation in CHEAR as a TAR, will enable the successful and smooth implementation of HHEAR exposomics for the range of exposures that require rigorous analytical quality control.