A New Molecular Mechanism to Bioengineering a Liver

肝细胞移植有许多潜在的应用。广泛的动物 实验表明移植到肝脏或异位部位的肝细胞 生存，功能，并积极参与再生过程。然而我们的 了解肝细胞植入及其显著的增殖和 再生潜力是有限的，即使原代肝细胞移植是在 在治疗遗传性和获得性人类疾病的应用的门槛。我们 先前偶然观察到，正常肝细胞移植到 患有致死性肝病的动物的腹膜腔迁移到淋巴系统 并设计异位肝脏样类器官，以拯救动物模型免受致命伤害 代谢紊乱肝细胞如何进入肝细胞， 导致异位肿块产生的机制尚不清楚。我们 假设肝细胞必须借用一些分子机制， 淋巴细胞用于迁移到淋巴细胞中。我们的兴趣将是研究异位细胞 移植和我们的应用程序的中心目标是翻译一个高度 有趣的观察，异位肝脏的产生，以潜在的临床应用 对于肝病患者。

Hepatocyte transplantation has many potential applications. Extensive animal experiments have shown that hepatocytes transplanted in the liver or at ectopic sites survive, function, and actively participate in the regenerative process. However our understanding of hepatocyte engraftment and their remarkable proliferative and regenerative potential is limited, even if primary hepatocyte transplantation is at the doorstep of applications in the treatment of inherited and acquired human diseases. We previously made a serendipitous observation that normal hepatocytes transplanted in the peritoneal cavity of an animal with lethal liver disease migrate into the lymphatic system and engineer ectopic liver-like organoids that rescue an animal model from a fatal metabolic disorder. How hepatocytes enter the lymphatics and what molecular mechanism is responsible for the generation of ectopic mass is not known. We hypothesized that hepatocytes must borrow some of the molecular mechanism lymphocytes use to migrate into the lymphatics. Our interest will be to study ectopic cell transplantation and our central objective of our application is to translate a highly interesting observation, the generation of ectopic liver, to a potential clinical application for patients with liver diseases.