HEMATOPOIETIC STEM CELL DERIVED HEPATOCYTES

造血干细胞衍生的肝细胞

• 批准号: 6437138
• 负责人: ERIC LAGASSE
• 金额: $ 22.5万
• 依托单位: STEMCELLS, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437138
• 关键词: cell differentiation; genetically modified animals; hematopoiesis; hematopoietic stem cells; hematopoietic tissue transplantation; histogenesis; human tissue; laboratory mouse; liver cells; liver regeneration; single cell analysis; stem cell transplantation

DESCRIPTION (provided by applicant): The goal of this research program is to identify the mechanisms that allow hematopoietic stem cells (HSC) to differentiate into hepatocytes. The project detailed in this proposal is based on a mouse model for liver regeneration. Specifically we propose: 1) To test whether a single, highly purified HSC can differentiate into both the hematopoietic and hepatic lineage. 2) To conduct comparative studies between highly purified HSC from different sources with the aim of defining the best source of HSC for hepatic engraftment. 3) To examine whether hematopoietic engraftment is an obligatory step for hepatocyte differentiation. 4) Characterization of a transitional intermediate cell between HSC and hepatocytes. 5) To test whether HSC can contribute to other non-hematopoietic tissues. 6) To test whether human HSC can differentiate into hepatocytes. These discoveries together enable the identification of key factors for HSC plasticity. We propose that HSC-derived hepatocytes could be the basis for the development of cell-based therapeutics addressing a myriad of devastating human liver diseases.

描述（由申请人提供）：