LIVER STEM CELLS AND HUMANIZED MICE: A NEW MODEL FOR HCV

描述：本申请提出使用移植的NOD/SCID小鼠 （脾内或肾包膜下）与人肝细胞进行HCV检测 感染该项目将首先确定肝细胞亚群， 最容易感染HCV，并检查可能的影响 肝细胞生长因子（HGF）对移植小鼠肝细胞生长的影响。 同样的方法也适用于富马酰乙酰乙酸水解酶（FAH） 小鼠富集移植的人肝细胞。对于所有的优化 丁型肝炎病毒（HDV）将用作替代病毒， 丙肝病毒。最终，这些人源化小鼠将被测试HCV感染。 拟议商业应用：不可用

DESCRIPTION: This application proposes to use NOD/SCID mice transplanted (intrasplenically or under kidney capsule) with human hepatocytes for HCV infection. The project will first identify hepatocyte subpopulations that are most susceptible to HCV infection, and examine the possible effects of hepatocyte growth factor (HGF) on hepatocyte growth in the transplanted mice. The same approach will also be applied to fumarylacetoacetate hydrolase (FAH) mice to enrich transplanted human hepatocytes. For all of the optimization procedures, hepatitis delta virus (HDV) will be used as a surrogate virus for HCV. Eventually, these humanized mice will be tested for HCV infections. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE