LIVER STEM CELLS AND HUMANIZED MICE: A NEW MODEL FOR HCV

描述：该应用程序建议使用NOD/SCID小鼠移植 (脾内或肾被膜下)与人肝细胞联合检测丙型肝炎病毒 感染。该项目将首先确定符合以下条件的肝细胞亚群 最容易感染丙型肝炎病毒，并检查 肝细胞生长因子对移植小鼠肝细胞生长的影响。 同样的方法也将应用于富马酸乙酰乙酸酯水解酶(FAH)。 小鼠浓缩移植的人肝细胞。对于所有的优化 程序，丁型肝炎病毒(HDV)将被用作替代病毒 丙型肝炎。最终，这些人源化的小鼠将接受丙型肝炎病毒感染测试。 建议的商业应用：不可用

DESCRIPTION: This application proposes to use NOD/SCID mice transplanted (intrasplenically or under kidney capsule) with human hepatocytes for HCV infection. The project will first identify hepatocyte subpopulations that are most susceptible to HCV infection, and examine the possible effects of hepatocyte growth factor (HGF) on hepatocyte growth in the transplanted mice. The same approach will also be applied to fumarylacetoacetate hydrolase (FAH) mice to enrich transplanted human hepatocytes. For all of the optimization procedures, hepatitis delta virus (HDV) will be used as a surrogate virus for HCV. Eventually, these humanized mice will be tested for HCV infections. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE