HEMATOPOIETIC STEM CELL DERIVED HEPATOCYTES

描述（由申请人提供）： 这项研究计划的目标是确定机制， 造血干细胞（HSC）分化为肝细胞。 项目 本提案中详细介绍的是基于小鼠模型的肝再生。 具体而言，我们建议：1）测试单个高度纯化的HSC是否可以 分化成造血和肝细胞系。 2)进行 不同来源的高纯度HSC的比较研究 目的是确定用于肝移植的HSC的最佳来源。3)到 检查造血植入是否是肝细胞的必要步骤 分化 4)过渡性中间细胞的特征 HSC和肝细胞之间的关系。 5)为了测试HSC是否可以促进其他 非造血组织。 6)为了测试人类HSC是否可以分化为 转化成肝细胞 这些发现共同使识别HSC的关键因素成为可能 可塑性 我们认为HSC来源的肝细胞可能是 基于细胞的治疗方法的发展， 人类肝脏疾病

DESCRIPTION (provided by applicant): The goal of this research program is to identify the mechanisms that allow hematopoietic stem cells (HSC) to differentiate into hepatocytes. The project detailed in this proposal is based on a mouse model for liver regeneration. Specifically we propose: 1) To test whether a single, highly purified HSC can differentiate into both the hematopoietic and hepatic lineage. 2) To conduct comparative studies between highly purified HSC from different sources with the aim of defining the best source of HSC for hepatic engraftment. 3) To examine whether hematopoietic engraftment is an obligatory step for hepatocyte differentiation. 4) Characterization of a transitional intermediate cell between HSC and hepatocytes. 5) To test whether HSC can contribute to other non-hematopoietic tissues. 6) To test whether human HSC can differentiate into hepatocytes. These discoveries together enable the identification of key factors for HSC plasticity. We propose that HSC-derived hepatocytes could be the basis for the development of cell-based therapeutics addressing a myriad of devastating human liver diseases.