Defining the conformational control of nitric oxide synthases by a multipronged approach

通过多管齐下的方法定义一氧化氮合酶的构象控制

• 批准号: 10385652
• 负责人: Changjian Feng
• 金额: $ 10.5万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10385652
• 关键词: Administrative Supplement; Alzheimer' s Disease; Anabolism; Award; Bioinorganic Chemistry; Complex Mixtures; Development; Disease; Environment; Equipment; Family; Financial Support; Funding; Institution; Investments; Laboratories; Lead; Liquid Chromatography; Methods; Molecular Conformation; Multienzyme Complexes; New Mexico; Nitric Oxide; Nitric Oxide Synthase; Parents; Pharmacy facility; Process; Production; Productivity; Proteins; Research; Research Personnel; Site; Stroke; System; Techniques; United States National Institutes of Health; Universities; Work; biophysical chemistry; college; effective therapy; fast protein liquid chromatography; instrumentation; milligram; novel therapeutic intervention; novel therapeutics; parent project; programs; protein complex; protein purification; spectroscopic survey; unnatural amino acids

PROJECT SUMMARY The parent award is on deciphering conformational dynamics of nitric oxide synthase proteins through an integrated approach using advanced spectroscopic techniques. Detailed spectroscopic studies require milligram amounts of highly purified protein. Fast protein liquid chromatography (FPLC) is a form of liquid chromatography used to purify a target protein from a complex mixture. Our current FPLC has been a workhorse, but is near the end of operational lifetime. This administrative supplement is being requested to allow acquisition of a state-of-the-art ÄKTA pure 25 system. It has direct relevance to and immediate utility in enhancing our laboratory’s protein purification capabilities. As such, this equipment will greatly enhance our research productivity for the duration of the project, and will help us maintain a strong research program. Importantly, the unique capabilities of the ÄKTA pure 25 system will be indispensable for our efforts to develop and optimize methods for site-specifically incorporating unnatural amino acids into large, complex proteins with high fidelity, yield, and purity. Our findings will therefore be of great utility for other investigators using this process for other large, complex enzymes. Therefore, the requested equipment will help the parent project to exert a sustained, powerful influence on the research fields of bioinorganic and biophysical chemistry. Moreover, the administrative supplement will enhance the scientific environment for NIH-funded investigators at the University of New Mexico, both within and outside of the College of Pharmacy. To our knowledge, no comparable instrumentation exists in educational institutions within the region. Dean Godwin has committed substantial financial support to this supplement request since this is a great investment for the College. Taken as a whole, the proposed supplement will increase the parent award’s overall impact within the original scope of award, as well as bolster research quality and depth at our institution.

项目总结 父项奖是关于通过一种新的方法破译一氧化氮合酶蛋白的构象动力学。 采用先进光谱技术的综合方法。详细的光谱研究需要 毫克量的高纯度蛋白质。快速蛋白质液相色谱(FPLC)是一种液体 用于从复杂混合物中提纯目标蛋白质的层析法。我们目前的FPLC已经是一个 主力，但已接近使用寿命的末尾。本行政副刊的要求是 允许购买最先进的？KTA PURE 25系统。它对以下方面有直接的相关性和直接的效用 增强我们实验室的蛋白质提纯能力。因此，这种设备将极大地增强我们的 在项目期间的研究生产力，并将帮助我们保持一个强大的研究计划。 重要的是，KTA PURE 25系统的独特功能对于我们的开发工作将是不可或缺的 并优化将非天然氨基酸定点结合到大而复杂的蛋白质中的方法 高保真度、高产量和高纯度。因此，我们的发现将对使用这一方法的其他调查人员非常有用 其他大型、复杂的酶的加工过程。因此，所请求的设备将帮助父项目 对生物无机化学和生物物理化学的研究领域产生持续、强大的影响。 此外，行政副刊将为NIH资助的调查人员改善科学环境 在新墨西哥大学，药学院内外都有。据我们所知，没有 该区域内的教育机构也有类似的工具。迪恩·戈德温承诺 由于这对学院来说是一项巨大的投资，因此对这项补充请求提供了大量的财政支持。已被占用 总体而言，拟议的补编将在原范围内增加父奖项的整体影响 奖励，以及支持我们机构的研究质量和深度。