Defining the conformational control of nitric oxide synthases by a multipronged approach

通过多管齐下的方法定义一氧化氮合酶的构象控制

• 批准号: 10571224
• 负责人: Changjian Feng
• 金额: $ 8.91万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10571224
• 关键词: Administrative Supplement; Alzheimer' s Disease; Award; Bioinorganic Chemistry; Computer software; Development; Disease; Environment; Equipment; Family; Financial Support; Fourier Transform; Funding; Institution; Investigation; Investments; Methodology; Methods; Molecular Conformation; New Mexico; Nitric Oxide Synthase; Parents; Performance; Pharmacy facility; Protein Dynamics; Proteins; Research; Research Personnel; Resolution; Sampling; Site; Spectroscopy, Fourier Transform Infrared; Speed; Stroke; System; Techniques; United States National Institutes of Health; Universities; biophysical chemistry; college; design; effective therapy; infrared spectroscopy; instrument; instrumentation; novel therapeutics; parent project; protein complex

PROJECT SUMMARY The parent award is on deciphering conformational dynamics of nitric oxide synthase proteins through an integrated approach using advanced spectroscopic techniques. Fourier transform infrared (FT IR) spectroscopy is essential to the proposed studies of short-range conformational sampling (Aims 2 and 3) in the parent project. Here, we request funds to purchase a state-of-the-art Bruker VERTEX 80 FT IR spectrometer to enhance our IR research capabilities. Combining high sensitivity, resolution and speed with enhanced expandability and easy-to-use FT IR software, the VERTEX 80 system is the answer for demanding protein IR research. The high-performance research-grade instrument is designed to be top of the line. The superior capabilities of the system will be necessary for our efforts to develop and optimize our workflow and methods for site-specific IR spectroscopic investigation of local protein dynamics. Our findings will greatly benefit other investigators using IR methodologies for large, complex proteins. Therefore, the requested equipment will help the parent project to exert a sustained, powerful influence on the research fields of biophysics and bioinorganic chemistry. Moreover, the administrative supplement will enhance the scientific environment for NIH-funded investigators at the University of New Mexico, both within and outside of the College of Pharmacy. To our knowledge, no comparable instrumentation exists in educational institutions within the region. Dean Godwin has committed substantial financial support to this supplement request since this is a great investment for the College. Taken as a whole, the proposed supplement will increase the parent award’s overall impact within the original scope and bolster research quality and depth at our institution.

项目摘要 父母奖是通过一个解释一氧化氮合酶蛋白质构象动力学的 采用先进的光谱技术的综合方法。傅里叶变换红外（FT IR） 光谱学是必不可少的短程构象采样（目标2和3）的拟议研究中， 父项目。在此，我们申请资金购买最先进的布鲁克VERTEX 80 FT红外光谱仪， 提高我们的研究能力。结合高灵敏度、分辨率和速度， 可扩展性和易于使用的FT IR软件，VERTEX 80系统是对蛋白质IR要求苛刻的解决方案 research.高性能研究级仪器的设计是一流的。上级 该系统的能力对于我们努力开发和优化我们的工作流程和方法是必要的 用于局部蛋白质动力学的位点特异性红外光谱研究。我们的研究成果将大大有利于其他 研究人员使用IR方法研究大型复杂蛋白质。因此，所要求的设备将有助于 对生物物理学和生物无机学研究领域产生持续、强大影响的母项目 化学.此外，行政补充将改善NIH资助的科学环境， 研究人员在新墨西哥州的大学，无论是内部和外部的药学院。对我们 在知识方面，该区域的教育机构没有类似的工具。迪恩·戈德温 已承诺为这一补充请求提供大量财政支助，因为这是对 学院总的来说，拟议的补助金将在2010年内增加家长奖的总体影响。 原创范围和加强研究质量和深度在我们的机构。