KOMP2-Phase 2 Production and Phenotyping by the DTCC Consortium

在DTCC完成KOMP 2项目目标因COVID-19大流行而中断 UM 1 OD 023221行政补充 2021年8月1日至2022年7月31日 摘要 新出现的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的爆发 2020年初，KOMP 2项目的第10个年头，新冠肺炎疫情（COVID-19）导致 DTCC联盟成员在几个月的时间里进行了几乎所有的实地研究活动， 加州大学戴维斯分校和表型基因组学中心（TCP）。大流行期间的限制 减少小鼠繁殖和表型分析活动，以最大限度地提高物理距离， 以减轻病毒传播和扩散的方式运作。因此，在加州大学戴维斯分校工作， 在这一年的大部分时间里，TCP受到严重限制，仅限于维持低于预期的水平。 群体的繁殖水平和小鼠表型的减少。因此，DTCC 聚生体的表型分型少于品系总数。因此，DTCC财团 将无法用现有资金实现其表型分析目标，因此无法满足项目要求。 预期在2021年7月31日本项目年度结束前完成。适应 加州大学戴维斯分校和TCP的工作活动中断，科学和预算规划过程 在NIH也同样陷入了僵局，这阻止了它发布与KOMP相关的资金 2020年的机会公告。因此，DTCC-KOMP 2财团被拒绝 有机会申请有竞争力的续约。 此管理补充请求的目的是完成DTCC的生成 联盟有义务使用CRISPR/Cas9技术提供1000个突变小鼠品系， 进行表型分析，进行质量控制评估，冷冻保存种质， 并使老鼠和数据随时可供研究界使用。虽然目标是 生产和冷冻保存1000个突变小鼠品系已经达到了表型分析的目标， 不会的随着为应对疫情而实施的工作限制解除，预计 成人和胚胎表型鉴定管道将在本日历年晚些时候完全恢复。在 在这方面，对项目完成预测进行了调整， 在截至2022年7月31日的一年活动延长期内，所有KOMP 2-第2阶段项目目标。 因此，DTCC联盟请求行政支持，以获得为期1年的资助延期 KOMP 2-第2阶段赠款用于与母赠款具体目标有关的活动 活动，包括技术人员、表型分析用品、小鼠每日津贴和 维护成本、信息支持和项目协调。这些活动将使 完成26例早期成人纯合子和46例早期成人纯合子的队列生产和表型分型 成体杂合系、31个胚致死/亚存活系和8个晚成体纯合系。 此外，另外28个胚胎亚活力系将被复活，以产生用于 由于COVID-19而延迟和损坏的成像。完成所有这些活动将 使DTCC联合体能够履行其对KOMP 2-Phase 2项目的所有义务。

Completion of KOMP2 Project Goals Interrupted by the COVID-19 Pandemic at the DTCC UM1OD023221 Administrative Supplement August 1, 2021 – July 31, 2022 ABSTRACT The outbreak of emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) early in 2020, the 10th year of the KOMP2 Project, caused a suspension of all but essential in-person research activities for several months by DTCC Consortium members at UC Davis and The Centre for Phenogenomics (TCP). Restrictions during the pandemic curtailed mouse breeding and phenotyping activity in order to maximize physical distancing and operate in a way that mitigates viral transmission and spread. As a result, work at UC Davis and TCP was severely curtailed for most of the year, limited to maintaining a lower-than-expected level of breeding of cohorts and reduced phenotyping of mice. Consequently, the DTCC Consortium is short of the total number of lines phenotyped. Therefore, the DTCC Consortium will be unable to fulfill its phenotyping goals with available funds and thus will not meet project expectations by the end of the current project year on July 31, 2021. Commensurate with the disruption in work activities at UC Davis and TCP, scientific and budgetary planning processes were similarly stalled at NIH which prevented it from publishing a KOMP-related funding opportunity announcement in 2020. As a result, the DTCC-KOMP2 Consortium was denied the opportunity to apply for a competitive renewal. The aim of this administrative supplement request is to finish the generation of the DTCC Consortium obligation to deliver 1000 mutant mouse lines using CRISPR/Cas9 technology, perform phenotyping assays, conduct quality control assessments, cryopreserve germplasm, and make mice and data readily available to the research community. Although the goal of producing and cryopreserving 1000 mutant mouse lines has already met, the phenotyping goals will not be. As work restrictions imposed in response to the pandemic lift, it is expected that adult and embryo phenotyping pipelines will be fully restored later during this calendar year. In this context, projections for completion of the project have been adjusted to enable completion of all KOMP2-Phase2 project goals during a 1-year extension of activities ending July 31, 2022. Therefore, the DTCC Consortium requests administrative support for a 1-year funded extension of the KOMP2-Phase2 grant for activities related to the Specific Aims of the Parent Grant activities, including technical personnel, phenotyping supplies, mouse per diem and maintenance costs, informatics support, and project coordination. These activities will enable completion of cohort production and phenotyping of 26 early adult homozygous and 46 early adult heterozygous lines, 31 embryo lethal/subviable lines, and 8 late adult homozygous lines. In addition, another 28 embryo subviable lines will be reanimated to generate embryos for imaging that were delayed and spoiled due to COVID-19. Completion of all these activities will enable the DTCC Consortium to fulfill all of its obligations to the KOMP2-Phase2 project.