New Mexico Center for Metals in Biology and Medicine - Equipment Supplement

行政支持。用于NIGMS资助的中心和核心设施的设备采购-GM-21-029 摘要无机金属长期以来一直是新墨西哥州生物医学研究的重点，因为 与采矿有关的金属污染物在该区域的流行情况。在新墨西哥大学研究中心 生物和医学中的金属(UNM CMBM)，我们的主要关注点是研究 金属污染物以及利用金属的化学性质进行治疗和营养 目的。对金属物种和生物分子之间的分子相互作用的详细知识可以导致 到临床和人口层面的干预措施，以改善公共卫生。为此，我们建立了一个 新的综合分子分析核心(IMAC)提供成员，以及UNM健康科学中心 校园，拥有尖端的分析化学仪器和技术。正在发射，仍处于第一次发射 去年，用户的兴趣和活跃度激增。在回应我们研究界的需求时， 几种特定的分析应用需要额外的专门仪器来进行高精度的 对分子定量进行了讨论。为会员提供一台能够 处理目标代谢组和脂组分析，以及检查DNA损伤、氧化生物标志物、 和金属相关的生物分子，Sciex 5500 Triple QuadTM提供了一种极具成本效益的系统来 满足我们多样化的量化需求。具有高度的灵敏度、选择性和线性动态范围， 它允许进行最苛刻的量化分析，例如对严重干扰的低水平的挑战 在分析特定代谢物和生物标志物时经常遇到的痕量量化 生物和环境样本。三重四次LC-MS/MS系统提供卓越的定量结果 单一注塑工作流程。通过对已知小分子提供可靠的定向定量分析，例如 代谢物、生物标记物和感兴趣的多肽，它是对我们的 Orbitrap LC-HRMS平台，优先用于非靶向蛋白质分析。这是最先进的 该系统是我们HSC校园中的第一个此类系统。它将补充我们现有的MS系统，以提供新的 以及针对我们用户群的扩展应用程序。此外，更快、更灵敏的仪器将有助于我们的 不断开发创新的生物分析方法，以满足我们用户的新研究需求。 在联合国监测机制建立这一能力肯定会满足更广泛受众的需求，并扩大 我们为UNM HSC准备的iMac。因此，5500系统将加强我们的用户访问和影响 资源，提高实验室的生产力和效率，并帮助维持核心。

Administrative Suppl. for Equipment Purchases for NIGMS-funded Center and Core Facilities NOT-GM-21-029 Summary Inorganic metals have long been a focus of biomedical research in New Mexico, due to the high prevalence of mining-related metal contaminants in the region. At the University of New Mexico Center for Metals in Biology and Medicine (UNM CMBM), our main focus is on both studying adverse health outcomes of metal contaminants as well as on harnessing the chemical properties of metals for therapeutic and nutritional purposes. Detailed knowledge of molecular interactions between metallic species and biomolecules can lead to clinical and population-level interventions to improve public health. Towards this end, we have established a new Integrative Molecular Analysis Core (IMAC) to provide members, and the UNM Health Sciences Center campus, with cutting edge analytical chemistry instrumentation and techniques. is launching, still in its first year, and seeing a surge of user interest and activity. In responding to needs of our research community, several specific analytical applications require additional specialized instrumentation for high-accuracy small molecule quantitation have been discussed. To provide the membership with a workhorse machine that can handle targeted metabolomic and lipidomic assays, as well as examine DNA damage, oxidative biomarkers, and metal-associated biomolecules, the Sciex 5500+ Triple QuadTM offers a superb cost-effective system to meet our diverse quantitation needs. Featuring a high level of sensitivity, selectivity and linear dynamic range, it allows for the most demanding quantitation analyses, such as challenges of severely interfered low-level trace quantification that are commonly encountered during analysis of specific metabolites and biomarkers in biological and environmental samples. Triple Quad LC-MS/MS systems deliver superior quantitative results in a single injection workflow. By offering robust targeted quantitative analysis of known small molecules such as metabolites, biomarkers and peptides of interests, it is a highly beneficial addition and expansion to our Orbitrap LC-HRMS platform that is preferentially used for non-targeted protein analysis. This state-of-the-art system is the first of its kind on our HSC campus. It will complement our current MS systems to provide new and extended applications for our user base. Moreover, faster and more sensitive instrument will help with our constant development of innovative bioanalytical methods to meet new research needs of our users. Establishing this capacity at UNM will certainly meet the needs of a wider audience and expand the value of our IMAC for the UNM HSC. Therefore, the 5500+ system will enhance our user access to and impact of the resource, improve the productivity and efficiency in the laboratory, and help sustain the Core.