The National Center for Microscopy and Imaging Research, a Community-wide Scientific Resource

国家显微镜和成像研究中心，社区范围的科学资源

• 批准号: 10400847
• 负责人: Mark H Ellisman
• 金额: $ 62.91万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400847
• 关键词: Advisory Committees; Aging; Biological; Biomedical Research; Biomedical Technology; California; Cells; Communities; Complex; Cryoelectron Microscopy; Data; Development; Disease; Elements; Evaluation; Fruit; Funding; Goals; Guidelines; Health; Heart; Home; Image; Imaging technology; Infrastructure; Institutes; Institution; Light; Macromolecular Complexes; Maintenance; Methods; Microscopic; Microscopy; Mission; Modeling; Molecular Probes; Molecular Structure; National Institute of General Medical Sciences; Organ; Performance; Physiological Processes; Preparation; Procedures; Process; Recovery; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Resource Allocation; Resources; Services; Specimen; Structure; Structure-Activity Relationship; Surface; Techniques; Technology; TimeLine; Tissues; Universities; Ursidae Family; base; community engagement; computerized tools; cost; insight; instrumentation; macromolecule; microscopic imaging; novel; operation; particle; programs; quality assurance; reconstruction; technology development; technology research and development; tool

PROJECT SUMMARY AND ABSTRACT The National Center for Microscopy and Imaging Research (NCMIR) at UC San Diego is a vital and unique NIGMS supported biomedical technology development research resource (BTRR). NCMIR is completing its 31st and final funded year of continuous operation and is thereby ineligible for renewal under new guidelines governing the P41 program. With this proposal, we request stabilizing support to help ease the transition of the Center to independent sustainability and to allow for the ordered completion of the resource's existing pool of supported biomedical research projects. NCMIR's mission is to advance the coordinated use of specialized molecular probes, novel specimen preparation methods, leading-edge microscopy and imaging technologies, and scalable computational tools to help investigators traverse difficult to navigate spatial and temporal scales, deliver new insight into multiscale structure/function relationships, and provide a fundamental understanding of the macromolecular mechanisms underlying many topics in health, disease, and aging. In transitioning the resource to a self-sustaining community-wide scientific resource, we provide details of our `Operation and Maintenance Plan' to govern how the resource supports ongoing research users, prepares developed technologies for broad dissemination, and modifies its approach to community engagement to communicate offered technologies, services, and resources. We further establish a `Transition Plan' to scale existing cost- recovery processes and put into place new mechanisms to stabilize support for the operation of the resource, buttressed by commitments from stakeholders within our home institution at the University of California, San Diego and the greater La Jolla mesa. Last, we establish an `Evaluation Plan' to quantify and assess resource performance and impact on the community, putting forth both Short-term Internal Metrics, to optimize resource utilization and make ongoing improvements, and Long-term Objective Metrics, to gauge overall impact on the scientific community.

项目总结和摘要 加州大学圣地亚哥分校的国家显微镜和成像研究中心（NCMIR）是一个重要而独特的 NIGMS支持生物医学技术开发研究资源（BTRR）。NCMIR正在完成其第31个 和最后一年的资金连续运作，因此没有资格根据新的指导方针进行续期 P41项目的管理通过这一提议，我们要求提供稳定支持，以帮助缓解过渡时期的困难。 中心独立的可持续性，并允许有序完成资源的现有池， 支持生物医学研究项目。NCMR的使命是促进协调使用专门的 分子探针，新的样品制备方法，尖端的显微镜和成像技术， 和可扩展的计算工具，以帮助调查人员穿越难以导航的空间和时间尺度， 提供对多尺度结构/功能关系的新见解，并提供对 健康、疾病和衰老中许多主题的大分子机制。在过渡过程中， 资源，以自我维持的社区范围内的科学资源，我们提供了我们的详细信息`操作和 维护计划“，以管理资源如何支持正在进行的研究用户，准备制定 技术的广泛传播，并修改其方法，以社区参与沟通 提供技术、服务和资源。我们还制定了一个“过渡计划”，以调整现有成本- 恢复进程，并建立新的机制，以稳定对资源运作的支持， 在加州大学旧金山分校的利益相关者的支持下， 迭戈和大拉霍亚梅萨。最后，我们建立了一个“评估计划”，以量化和评估资源 绩效和对社会的影响，提出短期内部审计，以优化资源 利用和不断改进，以及长期目标，以衡量对 科学界。

项目成果

Structure versus function: Are new conformations of pannexin 1 yet to be resolved?

• DOI: 10.1085/jgp.202012754
• 发表时间: 2021-05-03
• 期刊: The Journal of general physiology
• 作者: Mim C;Perkins G;Dahl G
• 通讯作者: Dahl G

(-)-Epicatechin induces mitochondrial biogenesis and markers of muscle regeneration in adults with Becker muscular dystrophy.

• DOI: 10.1002/mus.27108
• 发表时间: 2021-03
• 期刊: Muscle & nerve
• 影响因子: 3.4
• 作者: McDonald CM;Ramirez-Sanchez I;Oskarsson B;Joyce N;Aguilar C;Nicorici A;Dayan J;Goude E;Abresch RT;Villarreal F;Ceballos G;Perkins G;Dugar S;Schreiner G;Henricson EK
• 通讯作者: Henricson EK

cAMP/PKA pathway and mitochondrial protection in oxidative stress-induced optic nerve head astrocytes.

• DOI: 10.4103/1673-5374.286962
• 发表时间: 2021-01
• 期刊: Neural regeneration research
• 影响因子: 6.1
• 作者: Kim KY;Ju WK
• 通讯作者: Ju WK

Lamin B1 overexpression alters chromatin organization and gene expression.

• DOI: 10.1080/19491034.2023.2202548
• 发表时间: 2023-12
• 期刊: Nucleus (Austin, Tex.)
• 作者: Kaneshiro JM;Capitanio JS;Hetzer MW
• 通讯作者: Hetzer MW

Spatially resolved correlative microscopy and microbial identification reveal dynamic depth- and mineral-dependent anabolic activity in salt marsh sediment.

• DOI: 10.1111/1462-2920.15667
• 发表时间: 2021-08
• 期刊: Environmental microbiology
• 影响因子: 5.1
• 作者: Marlow J;Spietz R;Kim KY;Ellisman M;Girguis P;Hatzenpichler R
• 通讯作者: Hatzenpichler R