National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination Resource

项目摘要/摘要 该项目旨在传播国家显微镜中心经过验证的技术和资源 加州大学圣地亚哥分校的NCMIR和成像研究(NCMIR)推动大脑战略目标的完成 主动权。拟议的技术集成和传播资源将提供神经科学 社区提供技术帮助以获取和管理大规模数据，从而扩大访问和 在他们的研究中纳入了高通量多尺度成像工具和前沿分析策略。 将提供丰富的集成资源，包括分子探测器、成像平台和数据分析工具 这肯定会促进和加速大脑研究。我们将支持的研究项目类型包括：1) 需要穿越时空尺度来揭示对特定问题的新见解和理解的研究 神经群体；2)旨在标记和跟踪神经元过程和/或可视化目标的调查 在多种动物模型中，大量神经组织内局部环路的连接；3)投射 寻求对与以下相关的细胞和亚细胞水平的改变进行纳米组织学评估 学习、生理状态或疾病；以及4)执行更高分辨率3D形态分析的项目 功能的亚细胞基础，特别强调突触功能受 亚细胞成分。这个新中心将利用行政框架和信息技术 已经到位并经过多年改进的网络基础设施。除了提供 管理结构，该框架包括社区外展、项目审查、遴选和入职 由PI(和组装的团队)精炼和长期实践的流程。其他机制可用于 工具/技术传播和培训、数据管理和共享以及费用回收机制 还将利用和扩大长期可持续性，以最大限度地扩大我们的 技术和资源。

PROJECT SUMMARY/ABSTRACT This project aims to disseminate validated technologies and resources of the National Center for Microscopy and Imaging Research (NCMIR) at UC San Diego to advance the completion of strategic goals of the BRAIN Initiative. The proposed technology integration and dissemination resource will provide the neuroscience community with technical help to obtain and manage large scale data, broadening the access to and incorporation of high throughput multiscale imaging tools and leading-edge analysis strategies in their studies. Richly integrated resources will be offered, including molecular probes, imaging platforms and data analysis tools certain to enable and hasten brain research. The types of research projects we will support, include: 1) investigations requiring the traversal of spatiotemporal scales to reveal new insight and understanding of specific neural populations; 2) investigations which aim to mark and track neuronal processes and/or visualize targeted connectomes of local circuits within large volumes of nervous tissue in multiple animal models; 3) projects which seek to perform nano-histological assessment of cellular and subcellular level alterations associated with learning, physiological state, or disease; and 4) projects performing higher resolution 3D morphometric analysis of subcellular underpinnings of function, with particular emphasis on synaptic function as influenced by subcellular constituents. This new center will leverage an administrative framework and information technology cyberinfrastructure which is already in place and has been refined over many years. In addition to providing a management structure, this framework includes a community outreach, project review, selection, and onboarding process refined and long practiced by the PI (and the assembled team). Additional mechanisms for tool/technology dissemination and training, data management and sharing, and mechanisms for cost-recovery and long-term sustainability will also be leveraged and expanded so as to maximize the reach and impact of our technologies and resources.