National Center for Microscopy and Imaging Research: A BRAIN Technology Integration and Dissemination Resource

项目概要/摘要 该项目旨在传播国家显微镜中心的有效技术和资源 和成像研究（NCMIR）在加州大学圣地亚哥分校，以推进大脑的战略目标的完成 倡议拟议的技术集成和传播资源将提供神经科学 社区的技术帮助，以获得和管理大规模的数据，扩大访问和 高通量多尺度成像工具和前沿分析策略的结合。 将提供丰富的综合资源，包括分子探针，成像平台和数据分析工具 一定会促进大脑研究。我们将支持的研究项目类型包括：1） 调查需要穿越时空尺度，以揭示新的见解和理解的具体 2）旨在标记和跟踪神经元过程和/或可视化靶向的研究 在多种动物模型中，局部回路的连接体在大量神经组织内; 3）投射， 寻求对与以下相关的细胞和亚细胞水平变化进行纳米组织学评估： 学习、生理状态或疾病;以及4）执行更高分辨率3D形态测量分析的项目 功能的亚细胞基础，特别强调突触功能的影响 亚细胞成分这个新的中心将利用行政框架和信息技术 网络基础设施已经到位，并经过多年的完善。除了提供 管理结构，该框架包括社区外展、项目审查、选择和入职 PI（和组装的团队）对流程进行了细化和长期实践。其他机制 工具/技术传播和培训、数据管理和共享以及成本回收机制 和长期的可持续性也将得到利用和扩大，以最大限度地扩大我们的覆盖面和影响力， 技术和资源。

PROJECT SUMMARY/ABSTRACT This project aims to disseminate validated technologies and resources of the National Center for Microscopy and Imaging Research (NCMIR) at UC San Diego to advance the completion of strategic goals of the BRAIN Initiative. The proposed technology integration and dissemination resource will provide the neuroscience community with technical help to obtain and manage large scale data, broadening the access to and incorporation of high throughput multiscale imaging tools and leading-edge analysis strategies in their studies. Richly integrated resources will be offered, including molecular probes, imaging platforms and data analysis tools certain to enable and hasten brain research. The types of research projects we will support, include: 1) investigations requiring the traversal of spatiotemporal scales to reveal new insight and understanding of specific neural populations; 2) investigations which aim to mark and track neuronal processes and/or visualize targeted connectomes of local circuits within large volumes of nervous tissue in multiple animal models; 3) projects which seek to perform nano-histological assessment of cellular and subcellular level alterations associated with learning, physiological state, or disease; and 4) projects performing higher resolution 3D morphometric analysis of subcellular underpinnings of function, with particular emphasis on synaptic function as influenced by subcellular constituents. This new center will leverage an administrative framework and information technology cyberinfrastructure which is already in place and has been refined over many years. In addition to providing a management structure, this framework includes a community outreach, project review, selection, and onboarding process refined and long practiced by the PI (and the assembled team). Additional mechanisms for tool/technology dissemination and training, data management and sharing, and mechanisms for cost-recovery and long-term sustainability will also be leveraged and expanded so as to maximize the reach and impact of our technologies and resources.