Nathan Shock Center-San Antonio, Aperio VERSA 8 scanning microscope

这份行政补充文件的目的是请求拨款购买一台扫描显微镜 这将进一步改善内森休克中心病理学中心提供的核心服务 (NSC)在圣安东尼奥。病理学核心已经成为NSC的一部分约26年，以a）提供 为当地、国家和国际研究人员提供动物特征鉴定的病理学服务 衰老模型（小鼠、大鼠、裸鼹鼠和非人灵长类动物），和B）检查 对年龄相关组织病理学变化的各种干预（饮食、遗传和药物）。 病理学中心的服务可供其他中心的研究者使用，并已被其他中心的研究者使用 圣安东尼奥国家安全委员会，干预测试计划，圣安东尼奥，胡椒中心，其他 在UT健康部门，并在美国的其他学术机构。其中一个优势 SA休克中心是我们在啮齿动物详细病理分析方面的长期成就记录。 为专注于老龄化研究的研究人员提供殖民地，我们利用池野博士的专业知识 和Hubbard进行了以衰老为重点的组织病理学分析。病理学核心提供 在衰老动物模型（小鼠、大鼠、裸鼹鼠和非 人类灵长类动物），并使所得数据广泛提供给科学界。历史上， 老年病理学领域由于缺乏充分记载的病理学数据而受到阻碍;没有它， 存活数据仅提供了关于衰老的有限信息。此外，许多病理过程 通过营养和其他假定的衰老调节干预（例如，遗传和 药理学）。许多衰老研究缺乏病理学数据的一个主要原因是非常有限的 接触到在衰老研究方面有专长的病理学家。SA休克中心有一个长期的记录， 在啮齿动物群体的详细病理分析方面取得了成就，研究人员专注于衰老 池野博士和哈伯德博士进行的研究。除了衰老动物的病理学服务外， 模型（小鼠、大鼠、裸鼹鼠和非人类灵长类动物），病理学核心一直在开发 组织病理学数据和组织病理学切片图像的综合档案，可作为以下方面的资源： a）研究者的趋势分析;和B）新研究的基本病理学信息。此外，委员会认为， 病理学核心已经开发了一种包含石蜡和冷冻块的组织档案， 组织，我们提供额外的服务，使组织阵列载玻片和未染色的石蜡或 冷冻切片，进行组织学染色（包括特殊染色），并进行激光捕获 显微解剖 最近，病理学核心已经收到了许多询问，以提供“幻灯片自动扫描” 服务的扫描显微镜，因为新兴的兴趣和需求，以执行“空间 转录组学。”这种尖端和创新的方法变得重要，因为空间基因 表达数据结合单细胞RNAseq实验，该实验目前由Integrated 衰老核心生理学（IPAC），可以提供一个全面的基因表达谱。滑动自动- 病理核心沿着特殊转录组学的扫描显微镜扫描服务， 圣安东尼奥NSC的IPAC将促进从单细胞到组织切片的基因表达测定。 增加新设备的请求符合所述的支助目的， 加强共享资源和设施的分类研究，由一些调查员 来自不同的学科。圣安东尼奥国家安全委员会病理学核心与其他计划的整合 研究衰老过程的机制对这些研究有明显的好处。的 结合额外的扫描显微镜系统将允许我们提供2D基因表达 在老化过程中和通过各种干预的组织切片上的图像。

The purpose of this administrative supplement is to request funds to purchase a scanning microscope that will further improve the Core service provided by the Pathology Core of the Nathan Shock Center (NSC) in San Antonio. The Pathology core has been part of the NSC for about 26 years to a) provide pathology services to local, national, and international investigators for the characterization of animal models (mice, rats, naked mole-rats, and non-human primates) of aging, and b) examine the effects of various interventions (dietary, genetic, and pharmacological) on age-related histopathological changes. The service of the Pathology Core is available to and has been used by investigators from the other cores of the San Antonio NSC, the Intervention Testing Program, San Antonio, Pepper Center, other departments at UT Health, and at other academic institutions in the United States. One of the strengths of the SA Shock Center is our long record of accomplishment in detailed pathological analyses of rodent colonies for investigators focused on aging research, in which we capitalize on the expertise of Drs. Ikeno and Hubbard in the conduct of aging-focused histopathological analyses. The Pathology Core provides investigators with pathology services in aging animal models (mice, rats, naked mole-rats, and non- human primates) and makes the resulting data widely available to the scientific community. Historically, the biogerontology field has been hampered by a lack of well-documented pathology data; without it, survival data provide only limited information about aging. Furthermore, many pathological processes are modulated by nutritional and other putative aging-modulating interventions (e.g., genetic and pharmacological). A major reason for lack of pathology data in many aging studies has been very limited access to pathologists with expertise in aging research. The SA Shock Center has a long record of accomplishments in detailed pathological analyses of rodent colonies for investigators focused on aging research conducted by Drs. Ikeno and Hubbard. In addition to the pathology services in aging animal models (mice, rats, naked mole-rats, and non-human primates), the Pathology Core has been developing a comprehensive archive of histopathological data and images of histopathology slides as a resource for: a) trend analyses by investigators; and b) basic pathological information for new studies. Furthermore, the Pathology Core has developed a tissue archive containing paraffin and frozen blocks, and frozen tissues, with which we provide additional services to make tissue array slides and unstained paraffin or frozen sections, perform histological staining (including special staining), and perform laser capture microdissection. Recently, the Pathology Core has received numerous inquiries to provide “slide auto-scanning” service by the scanning microscope because of the emerging interest and demand to perform “Spatial Transcriptomics.” This cutting-edge and innovative approach becomes important because spatial gene expression data combined with single cell RNAseq experiment, which is currently provided by Integrated Physiology of Aging Core (IPAC), could provide a comprehensive gene expression profile. The slide auto- scanning service with this scanning microscope by Pathology Core along with special transcriptomics by IPAC of the San Antonio NSC will promote the gene expression assays from single cell to tissue sections. This request for additional new equipment conforms to the stated purpose of supporting and enhancing shared resources and facilities for categorical research by a number of investigators from different disciplines. The integration of the San Antonio NSC Pathology Core with other programs to study mechanisms of the aging process has been an obvious benefit to these studies. The incorporation of an additional scanning microscope system will allow us to provide 2D gene expression images on tissue sections during aging and by various interventions.