Nathan Shock Center-San Antonio, Aperio VERSA 8 scanning microscope

本行政副刊的目的是申请购买扫描显微镜的资金 这将进一步改善内森休克中心病理核心提供的核心服务 (NSC)在圣安东尼奥。病理核心已经成为NSC的一部分约26年了，以提供a) 为当地、国家和国际研究人员提供动物特征的病理学服务 衰老模型(小鼠、大鼠、裸鼠和非人灵长类动物)，以及b)检验 对与年龄相关的组织病理学变化的各种干预(饮食、遗传和药物)。 病理核心的服务可以提供给其他核心的调查人员，并已被其他核心的调查人员使用 圣安东尼奥国家科学中心，干预测试项目，圣安东尼奥，胡椒中心，其他 UT Health和美国其他学术机构的部门。其中一个优势是 SA休克中心是我们在啮齿动物详细病理分析方面的长期成就 殖民地研究人员专注于衰老研究，在这些研究中，我们利用了Ikeno博士的专业知识 和哈伯德在进行以衰老为重点的组织病理学分析。病理核心提供 在衰老动物模型(小鼠、大鼠、裸鼠和非 人类灵长类动物)，并将结果数据广泛提供给科学界。从历史上看， 生物老年学领域一直受到缺乏良好记录的病理数据的阻碍；如果没有这些数据， 存活数据只能提供有限的衰老信息。此外，许多病理过程是 由营养和其他假定的调节衰老的干预措施(例如，遗传和 药理学)。在许多衰老研究中缺乏病理学数据的一个主要原因是非常有限的 接触在衰老研究方面有专长的病理学家。SA休克中心有很长的记录 老龄化研究人员在啮齿动物群体详细病理分析方面的成就 由Ikeno和Hubbard博士进行的研究。除了老年动物的病理学服务 模型(小鼠、大鼠、裸鼠和非人类灵长类动物)，病理学核心一直在发展 组织病理学数据和组织病理学幻灯片图像的综合档案，作为以下资源： A)调查人员的趋势分析；以及b)新研究的基本病理信息。此外， 病理核心开发了一个包含石蜡和冰冻块的组织档案，并进行了冷冻 组织，我们用它提供额外的服务来制作组织阵列载片和未染色的石蜡或 冰冻切片，进行组织学染色(包括特殊染色)，并进行激光捕获 显微解剖。 最近，病理核心收到了许多关于提供“幻灯片自动扫描”的查询 扫描显微镜的服务是因为新兴的兴趣和执行空间的需求 转录学。“这种前沿和创新的方法变得重要，因为空间基因 结合单细胞RNAseq实验的表达数据，目前由Integrated提供 生理学的老化核心(IPAC)，可以提供一个全面的基因表达谱。滑梯自动- 这款扫描显微镜由病理核心提供扫描服务，并提供特殊转录本 圣安东尼奥国家科学中心的IPAC将推动从单细胞到组织切片的基因表达分析。 这项额外新设备的申请符合支持和 加强若干研究人员在分类研究方面的共享资源和设施 来自不同的学科。圣安东尼奥NSC病理核心中心与其他项目的整合 研究衰老过程的机理对这些研究具有明显的益处。这个 加入额外的扫描显微镜系统将使我们能够提供2D基因表达 在老化和各种干预下的组织切片上的图像。

The purpose of this administrative supplement is to request funds to purchase a scanning microscope that will further improve the Core service provided by the Pathology Core of the Nathan Shock Center (NSC) in San Antonio. The Pathology core has been part of the NSC for about 26 years to a) provide pathology services to local, national, and international investigators for the characterization of animal models (mice, rats, naked mole-rats, and non-human primates) of aging, and b) examine the effects of various interventions (dietary, genetic, and pharmacological) on age-related histopathological changes. The service of the Pathology Core is available to and has been used by investigators from the other cores of the San Antonio NSC, the Intervention Testing Program, San Antonio, Pepper Center, other departments at UT Health, and at other academic institutions in the United States. One of the strengths of the SA Shock Center is our long record of accomplishment in detailed pathological analyses of rodent colonies for investigators focused on aging research, in which we capitalize on the expertise of Drs. Ikeno and Hubbard in the conduct of aging-focused histopathological analyses. The Pathology Core provides investigators with pathology services in aging animal models (mice, rats, naked mole-rats, and non- human primates) and makes the resulting data widely available to the scientific community. Historically, the biogerontology field has been hampered by a lack of well-documented pathology data; without it, survival data provide only limited information about aging. Furthermore, many pathological processes are modulated by nutritional and other putative aging-modulating interventions (e.g., genetic and pharmacological). A major reason for lack of pathology data in many aging studies has been very limited access to pathologists with expertise in aging research. The SA Shock Center has a long record of accomplishments in detailed pathological analyses of rodent colonies for investigators focused on aging research conducted by Drs. Ikeno and Hubbard. In addition to the pathology services in aging animal models (mice, rats, naked mole-rats, and non-human primates), the Pathology Core has been developing a comprehensive archive of histopathological data and images of histopathology slides as a resource for: a) trend analyses by investigators; and b) basic pathological information for new studies. Furthermore, the Pathology Core has developed a tissue archive containing paraffin and frozen blocks, and frozen tissues, with which we provide additional services to make tissue array slides and unstained paraffin or frozen sections, perform histological staining (including special staining), and perform laser capture microdissection. Recently, the Pathology Core has received numerous inquiries to provide “slide auto-scanning” service by the scanning microscope because of the emerging interest and demand to perform “Spatial Transcriptomics.” This cutting-edge and innovative approach becomes important because spatial gene expression data combined with single cell RNAseq experiment, which is currently provided by Integrated Physiology of Aging Core (IPAC), could provide a comprehensive gene expression profile. The slide auto- scanning service with this scanning microscope by Pathology Core along with special transcriptomics by IPAC of the San Antonio NSC will promote the gene expression assays from single cell to tissue sections. This request for additional new equipment conforms to the stated purpose of supporting and enhancing shared resources and facilities for categorical research by a number of investigators from different disciplines. The integration of the San Antonio NSC Pathology Core with other programs to study mechanisms of the aging process has been an obvious benefit to these studies. The incorporation of an additional scanning microscope system will allow us to provide 2D gene expression images on tissue sections during aging and by various interventions.