Whole-Room Calorimeter

摘要 芝加哥大学(UChicago)转化医学研究所(ITM)提议购买和 安装大型灵活的间接量热计(IC)以支持新陈代谢、糖尿病、 肥胖、甲状腺功能障碍、微生物组、炎症性肠病、体重减轻、虚弱、 昼夜节律生物学和肾脏疾病等。IC是衡量能源消耗的黄金标准 (Ee)在受监管的环境条件下提供精确和准确的措施，范围从最大 体力活动至睡眠(SMR)。15年来，芝加哥大学的研究人员一直使用新陈代谢来测量EE 购物车和双标签水，仅测量有限持续时间内的静息EE或RMR(最多4 小时)，需要在头顶放置引擎盖，限制了自由移动，而且精度低于IC。尽管 这些限制，UChicago NIH资助的调查人员自2004年以来纳入了EE的措施，导致 极具影响力的科学发现。本申请中提出的IC是唯一可长期使用的设备 长期(24小时或更长时间)的EE测量，可以严格地确定昼夜EE(即RMR与SMR) 受控的环境条件，同时评估日常生活的方方面面，包括饮食或热效应 食物(TEF)、睡眠(SMR)和体力活动。随着2015年微生物组中心的成立 和2017年芝加哥大学的Duchossois家庭研究所，在卫生微生物群方面的世界领先项目 和疾病现在正在被证实。有强有力的证据表明，微生物群对EE的影响 各种复杂的医学疾病，如肥胖、厌食、代谢综合征、糖尿病、昼夜生物学、 慢性肾脏疾病、冠状病毒感染后19种综合征、充血性心力衰竭和炎症性肠病。 然而，与这些疾病相关的EE变化是细微差别的，在精确度和准确性方面存在局限性 需要更好的仪器。本申请中提出的IC的使用将有助于优化患者的长期 在各种医疗环境中的健康状况。通过在收集生物液的同时具有IC测量EE的能力 同时，在受控环境中，微生物组、新陈代谢和昼夜节律的贡献作用 生物学可以被刻画成。通过ITM，一个多机构的CTSA项目与拉什大学合作 并隶属于洛约拉大学、北岸医院、倡导中心和伊利诺伊州研究所 技术方面，将促进和支持IC在全市的使用。来自其他芝加哥的调查人员- 总部设在西北大学和伊利诺伊大学等机构以及区域或大湖等 作为威斯康星大学和营养肥胖研究中心(NORC)来自威斯康星大学的研究人员 密歇根州将进行科学调查。与来自其他站点的经验丰富的EE调查人员举行研讨会 将推进和传播该中心的科学成果，推动多个领域向前发展。

Abstract The Institute for Translational Medicine (ITM) at the University of Chicago (UChicago) proposes to purchase and install a large and flex indirect calorimeter (IC) to support leading research programs in metabolism, diabetes, obesity, disorders of thyroid function, the microbiome, inflammatory bowel disease, weight reduction, frailty, circadian biology, and kidney disorders, among others. IC is the gold standard for measuring energy expenditure (EE) providing precise and accurate measures under regulated environmental conditions, ranging from maximal physical activity to sleep (SMR). For over 15 years UChicago investigators have measured EE using metabolic carts and doubly-labelled water, which only measures resting EE or RMR, for a limited duration (maximum 4 hours), requiring an over-the-head hood placement, limiting free mobility and with less precision than IC. Despite these limitations, UChicago NIH-funded investigators have incorporated measures of EE since 2004 resulting in highly impactful scientific findings. The IC proposed in this application is the only equipment available for long term (24hrs or more) EE measurements, that can determine circadian EE (ie RMR vs. SMR) with strictly controlled environmental conditions while aspects of daily life are evaluated including eating or thermal effects of food (TEF), sleeping (SMR) and physical activity. With the establishment of the Microbiome Center in 2015 and the Duchossois Family Institute in 2017 at UChicago, world-leading programs in the microbiome in health and disease are now being established. There is strong evidence for the impact of the microbiome on EE in a variety of complex medical disorders such as obesity, anorexia, metabolic syndrome, diabetes, circadian biology, chronic kidney disease, post COVID 19 syndromes, congestive heart failure and inflammatory bowel disease. However, EE changes associated with these disorders are nuanced and limitations in precision and accuracy require better instrumentation. Use of the IC proposed in this application will help to optimize patient long-term health in a variety of medical settings. By having capacity for IC measurement of EE while collecting biofluids simultaneously in a controlled environment, the contributory roles of the microbiome, metabolism and circadian biology can be characterized. Through the ITM, a multi-institution CTSA program partnered with Rush University and affiliated with Loyola University, Northshore Hospital, Advocate Center and the Illinois Institute for Technology, city-wide utilization of the IC will be facilitated and supported. Investigators from other Chicago- based institutions including Northwestern University and University of Illinois, and regional or great lakes such as University of Wisconsin and the Nutrition Obesity Research Center (NORC) investigators from University of Michigan, will perform scientific investigations. Seminars with experienced investigators in EE from other sites will advance and disseminate scientific findings from this center and advance multiple fields forward.