Optical Methods for Lesion Activity Assessment

摘要 这项拟议研究的总体目标是开发用于临床的方法。 病变结构和活动性的评估。新的成像技术采用非 需要电离辐射来辅助龋病的诊断和治疗。它不是 足以简单地检测到龋损，需要评估病变的方法 并确定是否需要化学干预。损伤的测量 病变的结构、光学和尺寸的变化 脱水提供了在单次检查中评估病变活动性的可能性。 这一提议背后的中心假设是，存在结构性差异 活动和停止的病变之间的差异，这些差异可以用近乎 红外(NIR)成像方法。这项建议的目标将通过 具体目标如下：(1)检验近红外反射率测量的假设 在病变过程中脱水对病变结构和活性的变化更敏感 以及近红外成像可以用来评估细胞的活性 活体内的冠状龋损。(2)检验交叉极化OCT的假设 (CP-OCT)可用于评估小窝和小窝内病变结构和活性的变化 化学干预前后乳牙的裂隙情况。(3)测试 CP-OCT可用于评估牙根表面病变活动性的假设 并在体内监测保守干预的效果。 这项提议的成功将提供评估病变活动性的方法 一次检查和监测化学干预的效果，从而 能够使用新的干预疗法来避免空化和修复。

ABSTRACT The overall objective of this proposed research is to develop methods for the clinical assessment of lesion structure and activity. New imaging technologies employing non- ionizing radiation are needed to aid in caries diagnosis and management. It is not sufficient to simply detect caries lesions, methods are needed to assess the lesion activity and determine if chemical intervention is needed. Measurements of lesion structure and the optical and dimensional changes to lesions that occur during dehydration offer the potential for lesion activity assessment during a single examination. The central hypothesis underlying this proposal is that there are structural differences between active and arrested lesions and these differences can be quantified using near- IR (NIR) imaging methods. The objectives of this proposal will be achieved through the following specific aims: (1) To test the hypothesis that NIR reflectance measurements during lesion dehydration are more sensitive to changes in lesion structure and activity than existing methods and that NIR imaging can be used to assess the activity of coronal caries lesions in vivo. (2) To test the hypothesis that cross-polarization OCT (CP-OCT) can be used to assess changes in lesion structure and activity in the pits and fissures of primary teeth before and after chemical intervention. (3) To test the hypothesis that CP-OCT can be used to assess the activity of lesions on root surfaces and monitor the efficacy of conservative intervention in vivo. Success of this proposal will provide methods for assessing the activity of lesions in a single examination and for monitoring the efficacy of chemical intervention, thereby enabling the use of new intervention therapies to avert cavitation and restoration.