Flow Cytometry

项目摘要 总结：流式细胞术和细胞分选仪核心设施配备了BD LSR II和BD FACSAria III流式细胞仪，为研究者提供了高速、高分辨率、高纯度和 细胞分选的可行性，以适应我们的COBRE研究人员的应用。此核心可帮助最终用户 他们的研究涉及传染病的研究。流式细胞仪和细胞分选仪的总体目标 核心设施是提供全面的流式细胞仪分析和分选的各种支持， 确保生物医学研究中心在宿主-病原体相互作用方面取得成功， COBRE初级调查员这一核心的建立也将促进协作和创新 在北达科他州的调查人员和学术研究团体成员中。该核心还将 教育我们的本科生和研究生的应用和当前的进展， 通过研讨会、讲座和实验室课程，学习流式细胞术和细胞分选技术。根据 主要目标是确保宿主病原体生物医学研究卓越中心的成功 我们的COBRE初级研究者的互动，流式细胞术和细胞分选仪的建立 因此，核心是必不可少的，以满足当前和未来的需求，联合国和邻近机构，并将 这对这项赠款的整体成功至关重要。因此，流式细胞术和细胞分选 核心计划作为UND SMHS的永久研究核心，我们将建立，发展和过渡到 在COBRE的15年支持期内，这种流式细胞术和细胞的主要作用 分类核心设施是：角色1：为我们的COBRE和其他生物医学研究人员提供各种 对全面流式细胞分析和分选的支持水平，包括仪器、技术 支持和咨询。角色2：教育教职员工，特别是研究生关于应用程序 以及流式细胞术和细胞分选技术的最新进展。

Project Summary Summary: The Flow Cytometry and Cell Sorter Core Facility, is equipped with a BD LSR II and a BD FACSAria III flow cytometer, which provide the investigators high speed, high resolution, high purity and viability of cell sorting to fit the application of our COBRE investigators. This core facilitates end users on their research involving the study of infectious disease. The overall goal of Flow Cytometry and Cell Sorter Core Facility is to provide various levels of support on comprehensive flow cytometric analysis and sorting, to ensure the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators. The establishment of this core will also foster collaboration and innovation among investigators and members of the academic research community in North Dakota. This core will also educate our undergraduate students and graduate students with the application and the current advances in flow cytometry and cell sorter technology by workshop, lectures and lab-based courses. According to the primary goal of ensuring the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators, establishment of the Flow Cytometry and Cell Sorter Core is therefore essential to meet the current and future needs of UND and neighboring institutions, and will be of great importance for the overall success of this grant. Therefore, The Flow Cytometry and Cell Sorting Core is planned as a permanent research core in the UND SMHS that we will establish, grow, and transit to independent core over a 15-year period of COBRE support. The major roles of this Flow Cytometry and cell sorting Core Facility are: Role 1: To provide our COBRE and other biomedical investigators with various levels of support on comprehensive flow cytometric analysis and sorting including instrumentation, technical support and consultation. Role 2: To educate faculty, staff, especially graduate students about the application and the current advances in flow cytometry and cell sorter technology.