Flow Cytometry

项目摘要 摘要：流式细胞仪和细胞分选仪核心设备配备了BD LSR II和BD FACSAria III流式细胞仪为研究人员提供了高速、高分辨率、高纯度和 细胞分选的可行性，以适应我们的Cobre研究人员的应用。此核心为终端用户提供便利 他们的研究涉及传染病的研究。流式细胞术和细胞分选仪的总体目标 核心设施是为全面的流式细胞仪分析和分类提供不同级别的支持，以 确保生物医学研究中心在宿主-病原体相互作用和 我们科布雷初级调查员的名单。这一核心的建立还将促进合作和创新 在北达科他州的调查人员和学术研究社区成员中。这个核心还将 教育我们的本科生和研究生，了解它的应用和当前在 流式细胞术和细胞分选技术通过研讨会、讲座和实验室课程进行。根据 确保生物医学卓越研究中心在宿主-病原体方面取得成功的首要目标 相互作用和我们Cobre初级研究人员，流式细胞仪和细胞分选仪的建立 因此，核心对于满足联合国开发计划署和邻近机构目前和未来的需要至关重要，并将 这对这笔赠款的全面成功非常重要。因此，流式细胞术和细胞分选 CORE计划作为联合国开发计划署SMHS的永久研究核心，我们将建立、发展和过渡到 独立核心，为期15年的科布雷支持。流式细胞术和细胞的主要作用 分类核心设施是：角色1：为我们的Cobre和其他生物医学研究人员提供各种 对全面的流式细胞仪分析和分类的支持程度，包括仪器、技术 支持和咨询。角色2：对教职员工，尤其是研究生进行有关应用程序的教育 以及目前在流式细胞术和细胞分选技术方面的进展。

Project Summary Summary: The Flow Cytometry and Cell Sorter Core Facility, is equipped with a BD LSR II and a BD FACSAria III flow cytometer, which provide the investigators high speed, high resolution, high purity and viability of cell sorting to fit the application of our COBRE investigators. This core facilitates end users on their research involving the study of infectious disease. The overall goal of Flow Cytometry and Cell Sorter Core Facility is to provide various levels of support on comprehensive flow cytometric analysis and sorting, to ensure the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators. The establishment of this core will also foster collaboration and innovation among investigators and members of the academic research community in North Dakota. This core will also educate our undergraduate students and graduate students with the application and the current advances in flow cytometry and cell sorter technology by workshop, lectures and lab-based courses. According to the primary goal of ensuring the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators, establishment of the Flow Cytometry and Cell Sorter Core is therefore essential to meet the current and future needs of UND and neighboring institutions, and will be of great importance for the overall success of this grant. Therefore, The Flow Cytometry and Cell Sorting Core is planned as a permanent research core in the UND SMHS that we will establish, grow, and transit to independent core over a 15-year period of COBRE support. The major roles of this Flow Cytometry and cell sorting Core Facility are: Role 1: To provide our COBRE and other biomedical investigators with various levels of support on comprehensive flow cytometric analysis and sorting including instrumentation, technical support and consultation. Role 2: To educate faculty, staff, especially graduate students about the application and the current advances in flow cytometry and cell sorter technology.