Center for Excellence In-Host Pathogen Interactions

宿主病原体相互作用卓越中心

• 批准号: 10398585
• 负责人: David S. Bradley
• 金额: $ 25万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-13 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10398585
• 关键词: 16 year old; Administrative Supplement; Aging; Air; Alzheimer' s Disease; Bacteria; Bacterial Infections; Centers of Research Excellence; Communities; Computers; Confocal Microscopy; Data Storage and Retrieval; Development; Disease; Drug Addiction; Dyes; Educational workshop; Ensure; Equipment; Experimental Designs; Fluorochrome; Functional Imaging; Goals; Housing; Image; Image Analysis; Immune response; Immunologics; Label; Life; Lyme Disease; Malignant Neoplasms; Mental Depression; Microscope; Microscopy; National Institute of General Medical Sciences; Online Systems; Parasites; Parasitic infection; Physiological; Policies; Process; Protocols documentation; Publications; Research; Research Personnel; Resolution; Sampling; Schedule; Science; Services; Support Contracts; System; Technology; Training; Virus; Virus Diseases; Water; base; confocal imaging; data sharing; design; detector; education resources; epigenomics; experience; experimental study; imaging approach; imaging capabilities; instrument; instrumentation; member; microscopic imaging; pathogen; programs; repaired; response; tool; web site; webinar

Project Summary: This is an administrative supplement proposal in response to NIGMS Notice number (NOT- GM-21-029). The purpose of this supplement proposal is to replace an outdated confocal microscope in the Imaging Core with a state-of-the-art Leica Stellaris 5 confocal microscope to support and extend the goals of the Host-Pathogen COBRE program. Our existing 16-year old Zeiss 510 META confocal microscope, which reached its end of service life in 2016, is based on old technology and is beginning to experience repair issues. As a result, COBRE investigators are unable to undertake imaging approaches that are possible using the technology that exists in current state-of-the-art microscopes. Replacing this instrument with a Leica Stellaris 5 confocal microscope will ensure that investigators have the technology they require to support and advance their research goals. The overall goals of these projects are to understand the immunological processes and mechanisms that underly a range of host-pathogen interactions in diseases caused by viral, bacterial, and parasitic infections. All projects require high resolution, multichannel imaging of samples labeled with fluorochromes having excitation wavelengths ranging from 405 nm to 633 nm in fixed and live samples. Unlike the existing confocal microscope in the Imaging Core, which is effectively a 3-channel system with conventional PMT detectors, the Stellaris 5 confocal microscope is a prism-based four channel system, equipped with ultrasensitive Power HyD S detectors and offering an array of advanced imaging features including superresolution imaging. It will allow investigators to image samples labelled with four or more fluorescent labels, including dyes such as DAPI, which cannot be visualized using our existing instrument. The Stellaris 5 microscope will be integrated into our already functional Imaging Core in association with other imaging equipment and will take advantage of existing web-based scheduling, technical support, web-based data storage and sharing, system contract support, and a microscopic imaging service supported by telemicroscopy. Acquisition of this instrumentation will build institutionally on our very successful COBRE, INBRE, and DaCCoTA CTR programs. The Stellaris 5 confocal with superresolution and other advanced capabilities will advance biomedical science research capacity by Host-Pathogen COBRE investigators. It will also extend the research capacity of investigators affiliated with Epigenomics in Development and Disease COBRE, investigators affiliated with the DaCCoTA CTR, and other investigators in the UND SMHS, on the UND campus, and in the regional research community.

项目摘要：这是响应 NIGMS 通知号的行政补充提案（非 GM-21-029）。该补充提案的目的是取代过时的共聚焦显微镜 配备最先进的 Leica Stellaris 5 共焦显微镜的成像核心可支持和扩展以下目标： 宿主病原体 COBRE 程序。我们现有的已有 16 年历史的 Zeiss 510 META 共焦显微镜， 于 2016 年达到使用寿命，基于旧技术，开始出现维修问题。 因此，COBRE 研究人员无法采用可能使用的成像方法 当前最先进的显微镜中存在的技术。使用 Leica Stellaris 5 替换该仪器 共焦显微镜将确保研究人员拥有支持和推进所需的技术 他们的研究目标。这些项目的总体目标是了解免疫过程和 病毒、细菌和病毒引起的疾病中一系列宿主与病原体相互作用的机制 寄生虫感染。所有项目都需要对标记的样品进行高分辨率、多通道成像 固定样品和活样品中激发波长范围为 405 nm 至 633 nm 的荧光染料。不像 成像核心中现有的共焦显微镜，实际上是一个 3 通道系统 与传统 PMT 探测器相比，Stellaris 5 共焦显微镜是基于棱镜的四通道系统， 配备超灵敏 Power HyD S 探测器并提供一系列先进的成像功能 包括超分辨率成像。它将允许研究人员对带有四个或更多标记的样本进行成像 荧光标记，包括 DAPI 等染料，无法使用我们现有的仪器进行可视化。这 Stellaris 5 显微镜将与其他显微镜一起集成到我们已经正常运行的成像核心中 成像设备，并将利用现有的基于网络的调度、技术支持、基于网络的 数据存储和共享、系统合同支持以及显微成像服务 望远显微镜。收购该仪器将在制度上建立在我们非常成功的 COBRE 的基础上， INBRE 和 DaCCoTA CTR 计划。 Stellaris 5 共焦具有超分辨率和其他先进功能 能力将提高宿主病原体 COBRE 研究人员的生物医学科学研究能力。它将 还扩展了与发育和疾病表观基因组学相关的研究人员的研究能力 COBRE、隶属于 DaCCoTA CTR 的调查人员以及 UND SMHS 的其他调查人员， UND 校园和区域研究社区。