CORE D: BIOMARKER CORE

核心 D：生物标志物核心

• 批准号: 10468890
• 负责人: Peyton Jacob
• 金额: $ 17.51万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-19 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10468890
• 关键词: Aerosols; Alkaloids; Analytical Chemistry; Animals; Behavior; Biological; Biological Assay; Biological Markers; Chemicals; Collaborations; Communication; Complex; Development; Electronic cigarette; Emerging Tobacco Products; Exposure to; Fostering; Goals; Gold; Health; In Vitro; Individual; Laboratories; Lead; Mass Fragmentography; Measures; Methods; Poison; Protocols documentation; Regulation; Research; Research Personnel; Research Priority; Risk; Sampling; System; Tobacco; Tobacco use; Toxicology; analytical method; behavioral economics; biomarker development; experience; heated tobacco products; improved; interest; mass spectrometer; nicotine exposure; product development; tobacco exposure; tobacco products

PROJECT SUMMARY/ABSTRACT Exposure assessment is necessary for evaluating the health effects of tobacco use. Measuring exposure to toxic and addictive substances is important for achieving the integrative theme of this TCORS, that understanding combined health effects, behavior, and impact analysis will provide actionable information for regulation of and public communications about current and emerging tobacco products The Biomarker Core will contribute to this goal through three specific aims: (1) Provide analytical chemistry support for UCSF TCORS investigators; (2) Continue and expand cross-TCORS collaborations by making available state-of-the- art analytical methods to foster such collaborations; (3) Develop new biomarkers of exposure to tobacco products. The Biomarker Core will identify new biomarkers of exposure and potential harm for new and emerging tobacco products, in particular e-cigarettes and heat-not-burn (HNB) products. The Biomarker Core will support studies carried out by TCORS investigators by analysis of biofluid samples for biomarkers of exposure and potential harm (Projects 1-4), and by analyzing tobacco products (e-cigarettes and HNB products) and their aerosols (Projects 1-3) for harmful and potentially harmful chemicals (HPHCs). Dr. Jacob and his senior laboratory staff have more than 30 years' experience in supporting large-scale studies and developing new biomarkers and analytical methods. The Biomarker Core laboratory will measure tobacco alkaloids and a wide range of toxic substances and other components of interest in both products and their aerosols. The core will assist the investigators of Projects 1, 2, and 3 in implementing protocols that will generate aerosols for chemical analysis and for delivery to in vitro systems and animals for toxicology studies. The analytical methods employed by the Biomarker Core are mostly chromatographic–mass spectrometric (GC-MS and LC-MS/MS) performed on triple quadrupole mass spectrometers, generally considered the gold standard for quantitative analysis of complex biological samples. As in our current TCORS, the Biomarker Core also expects to use its methods in collaborations with other TCORS beyond UCSF. Providing state-of-the-art exposure assessment to the individual projects, developing improved exposure assessment measures, and identifying substances in new and emerging tobacco products and their aerosols that lead to toxic substance exposure could contribute to the development of product standards for potential reduced risk products, as well as advance the field of tobacco exposure assessment in general.

项目摘要/摘要 暴露评估对于评估烟草使用对健康的影响是必要的。测量暴露于 有毒和成瘾物质对于实现本TCORS的综合主题非常重要，即 了解综合的健康影响、行为和影响分析将提供可操作的信息 对于现有和新兴烟草产品的监管和公共宣传，Biomarker Core 将通过三个具体目标为这一目标做出贡献：(1)为加州大学旧金山分校提供分析化学支持 TCORS调查人员；(2)通过提供最新情况，继续和扩大跨TCORS合作 促进这种合作的ART分析方法；(3)开发接触烟草的新生物标记物 产品。Biomarker Core将确定暴露的新生物标记物以及新的和 新兴烟草产品，特别是电子烟和热不燃烧(HNB)产品。生物标志物的核心 将通过对生物流体样本进行生物标志物分析来支持TCORS调查人员进行的研究 暴露和潜在危害(项目1-4)，以及分析烟草产品(电子烟和HNB (项目1-3)有害和可能有害的化学品(HPHCs)。雅各布博士 他的高级实验室工作人员在支持大规模研究和 开发新的生物标志物和分析方法。Biomarker核心实验室将测量烟草 生物碱和广泛的有毒物质以及产品中感兴趣的其他成分 气雾剂。核心将协助项目1、2和3的调查人员执行将 产生用于化学分析的气雾剂，并将其输送到体外系统和动物进行毒理学研究。 Biomarker Core使用的分析方法主要是色质联用 (GC-MS和LC-MS/MS)在三重四极杆质谱仪上进行，通常被认为是金 复杂生物样品定量分析标准。就像我们目前的TCORS一样，Biomarker Core 还希望将其方法用于与加州大学旧金山分校以外的其他TCOR的合作。提供最先进的 对个别项目进行风险评估，制定改进的风险评估措施，以及 识别新出现的烟草产品及其气雾剂中导致有毒物质的物质 风险暴露也可能有助于为潜在的低风险产品制定产品标准。 作为烟草暴露评估领域的先行者。