CORE D: BIOMARKER CORE

核心 D：生物标志物核心

• 批准号: 10259844
• 负责人: Peyton Jacob
• 金额: $ 19.34万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-19 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10259844
• 关键词: Aerosols; Alkaloids; Analytical Chemistry; Animals; Behavior; Biological; Biological Assay; Biological Markers; Chemicals; Collaborations; Communication; Complex; Development; Electronic cigarette; Emerging Tobacco Products; Exposure to; Fostering; Goals; Gold; Health; In Vitro; Individual; Laboratories; Lead; Mass Fragmentography; Measures; Methods; Poison; Protocols documentation; Regulation; Research; Research Personnel; Research Priority; Risk; Sampling; System; Tobacco; Tobacco use; Toxicology; analytical method; behavioral economics; biomarker development; experience; heated tobacco products; improved; interest; mass spectrometer; nicotine exposure; product development; tobacco exposure; tobacco products

PROJECT SUMMARY/ABSTRACT Exposure assessment is necessary for evaluating the health effects of tobacco use. Measuring exposure to toxic and addictive substances is important for achieving the integrative theme of this TCORS, that understanding combined health effects, behavior, and impact analysis will provide actionable information for regulation of and public communications about current and emerging tobacco products The Biomarker Core will contribute to this goal through three specific aims: (1) Provide analytical chemistry support for UCSF TCORS investigators; (2) Continue and expand cross-TCORS collaborations by making available state-of-the- art analytical methods to foster such collaborations; (3) Develop new biomarkers of exposure to tobacco products. The Biomarker Core will identify new biomarkers of exposure and potential harm for new and emerging tobacco products, in particular e-cigarettes and heat-not-burn (HNB) products. The Biomarker Core will support studies carried out by TCORS investigators by analysis of biofluid samples for biomarkers of exposure and potential harm (Projects 1-4), and by analyzing tobacco products (e-cigarettes and HNB products) and their aerosols (Projects 1-3) for harmful and potentially harmful chemicals (HPHCs). Dr. Jacob and his senior laboratory staff have more than 30 years' experience in supporting large-scale studies and developing new biomarkers and analytical methods. The Biomarker Core laboratory will measure tobacco alkaloids and a wide range of toxic substances and other components of interest in both products and their aerosols. The core will assist the investigators of Projects 1, 2, and 3 in implementing protocols that will generate aerosols for chemical analysis and for delivery to in vitro systems and animals for toxicology studies. The analytical methods employed by the Biomarker Core are mostly chromatographic–mass spectrometric (GC-MS and LC-MS/MS) performed on triple quadrupole mass spectrometers, generally considered the gold standard for quantitative analysis of complex biological samples. As in our current TCORS, the Biomarker Core also expects to use its methods in collaborations with other TCORS beyond UCSF. Providing state-of-the-art exposure assessment to the individual projects, developing improved exposure assessment measures, and identifying substances in new and emerging tobacco products and their aerosols that lead to toxic substance exposure could contribute to the development of product standards for potential reduced risk products, as well as advance the field of tobacco exposure assessment in general.

项目总结/摘要 暴露评估对于评估烟草使用的健康影响是必要的。测量暴露于 有毒和成瘾物质对于实现TCORS的综合主题非常重要， 了解综合的健康影响、行为和影响分析将提供可操作的信息 对当前和新兴烟草产品的监管和公众沟通 我将通过三个具体目标来实现这一目标：（1）为UCSF提供分析化学支持 TCORS调查员;（2）通过提供最新的信息，继续并扩大跨TCORS合作。 艺术分析方法，以促进这种合作;（3）开发新的生物标志物暴露于烟草 产品.生物标志物核心将确定新的生物标志物的暴露和潜在的伤害， 新兴烟草产品，特别是电子烟和加热不燃烧（HNB）产品。生物标志物核心 将支持TCORS研究人员通过分析生物流体样本的生物标志物进行的研究， 暴露和潜在危害（项目1-4），并通过分析烟草产品（电子烟和HNB 产品）及其气溶胶（项目1-3）的有害和潜在有害化学品（HPHC）。雅各布博士 他的高级实验室工作人员在支持大规模研究方面拥有30多年的经验， 开发新的生物标志物和分析方法。生物标志物核心实验室将测量烟草 生物碱和广泛的有毒物质和其他成分的利益，在这两个产品及其 气溶胶核心将协助项目1、2和3的调查人员执行协议， 产生用于化学分析和递送到体外系统和动物用于毒理学研究的气溶胶。 生物标志物核心采用的分析方法主要是色谱-质谱法 （GC-MS和LC-MS/MS）在三重四极杆质谱仪上进行，通常被认为是金 用于复杂生物样品定量分析的标准品。在我们目前的TCORS中，生物标志物核心 还希望使用其方法与其他TCORS超越加州大学旧金山分校的合作。提供最先进的 对个别项目进行风险评估，制定更好的风险评估措施，以及 确定新的和新兴的烟草产品及其气溶胶中导致有毒物质的物质 暴露也有助于制定潜在风险降低产品的产品标准， 作为推进烟草暴露评估领域的一般。