MULTIPLE BLOOD FEEDING BY MOSQUITOES

该更新提议研究假设是频繁的主机 接触者和多次采血的Ae.埃及伊蚊在单个性腺增生性疾病中 循环是登革热(DEN)病毒传播的重要因素。 我们的初步数据显示，假设Ae。仅埃及伊蚊饲料 在每个产卵周期中一次是不正确的，并且严重低估了 这种蚊子传播疾病的可能性。因为多个 喂食会极大地增加蚊子感染或 传播病毒感染，更好地了解 媒介与宿主的接触是了解登革热病毒的基础 并可能有助于了解节肢动物传播的 一般的疾病。 我们试图研究Ae的作用。埃及伊蚊在洞穴中的多次取食行为 传输的细节越来越详细，在这样做的过程中，测试问题 是我们目前研究的改进。我们三年的进度报告 和艾先生一起学习。波多黎各和泰国的埃及伊蚊表明(1) 以人类为食的多次进食是很常见的，两餐之间的时间间隔 可能随DEN传播的变化而波动；(2)频繁 供血有助于蚊子的能量需求，因为雌性蚊子很少 以植物糖为饲料；以及(3) 可能影响媒介能力的昆虫学因素，因此， 病毒传播。本提案中描述的实验旨在 确定(1)通过以下方式确定社区内多餐的分配 确定埃及伊蚊吸血的确切对象(S)；(2) 营养相关性和生殖适合性与多个 摄食行为；以及(3)蚊子存活是否与 多次取食的频率和蚊子传播的季节动态。 从这些研究中收集的信息对人们来说很重要 通过病媒控制和基础研究参与疾病预防 关于虫媒病毒生态和进化的研究。

The research hypothesis for this renewal proposal is that frequent host contacts and multiple blood feeding by Ae. aegypti in a single gonotrophic cycle are important contributions to transmission of dengue (DEN) viruses. Our preliminary data show that the assumption that Ae. aegypti feed only once during each egg laying cycle is incorrect and grossly underestimates the potential for spread of disease by this mosquito. Because multiple feeding can greatly increase the chances for a mosquito to contract or transmit a viral infection, an improved understanding of the frequency of host contact by vectors is fundamental for understanding DEN virus transmission and may contribute to knowledge regarding arthropod-borne diseases in general. We seek to study the role of Ae. aegypti multiple feeding behavior in DEN transmission in increasing detail and, in so doing, to test questions that are refinements of our current research. Our Progress Report for 3 years of study with Ae. aegypti in Puerto Rico and Thailand indicates that (1) multiple feeding on humans is common, and the time interval between meals may fluctuate in concert with changes in DEN transmission; (2) frequent blood feeding contributes to mosquito energy needs because females rarely feed on plant sugars; and (3) there is geographic variation in entomological factors that may influence vectorial capacity and, thus, virus transmission. Experiments described in this proposal are designed to determine (1) the distribution of multiple meals within a community by identifying the exact person(s) from which Aedes aegypti imbibe blood; (2) nutritional correlates and reproductive fitness associated with multiple feeding behavior; and (3) if mosquito survival is correlated with the frequency of multiple feeding and seasonal dynamics of DEN transmission. information gathered from these studies will be important to people involved in disease prevention through vector control and in basic studies of arboviral ecology and evolution.