MULTIPLE BLOOD FEEDING BY MOSQUITOES

这项更新建议的研究假设是， 接触和多血喂养的Ae。埃及伊蚊在一个单一的生殖营养 循环是登革热（DEN）病毒传播的重要贡献。 我们的初步数据表明，假设Ae。仅埃及饲料 在每个产卵周期一次是不正确的，严重低估了 这种蚊子传播疾病的可能性。因为多个 喂食会大大增加蚊子感染的机会， 传播病毒感染，更好地了解 媒介与宿主接触是了解登革病毒的基础 传播，并可能有助于了解节肢动物传播 一般的疾病。 我们试图研究Ae的作用。DEN中埃及伊蚊的多次取食行为 传输越来越详细，并在这样做，以测试问题， 是对我们当前研究的改进三年来的进展报告 与Ae一起学习波多黎各和泰国的埃及人表明（1） 多次进食是很常见的，两餐之间的时间间隔 可能会随着DEN传播的变化而波动;（2）频繁 吸血有助于蚊子的能量需求，因为雌性蚊子很少 以植物糖为食;（3） 可能影响媒介能力的昆虫学因素，因此， 病毒传播本提案中描述的实验旨在 （1）确定一个社区内的多餐分布， 确定埃及伊蚊吸食血液的确切人员;（2） 营养相关性和生殖健康与多种 取食行为;（3）如果蚊子的生存与 多次摄食的频率和DEN传播的季节动态。 从这些研究中收集的信息对人们来说很重要， 通过病媒控制和基础研究参与疾病预防 虫媒病毒生态学和进化。

The research hypothesis for this renewal proposal is that frequent host contacts and multiple blood feeding by Ae. aegypti in a single gonotrophic cycle are important contributions to transmission of dengue (DEN) viruses. Our preliminary data show that the assumption that Ae. aegypti feed only once during each egg laying cycle is incorrect and grossly underestimates the potential for spread of disease by this mosquito. Because multiple feeding can greatly increase the chances for a mosquito to contract or transmit a viral infection, an improved understanding of the frequency of host contact by vectors is fundamental for understanding DEN virus transmission and may contribute to knowledge regarding arthropod-borne diseases in general. We seek to study the role of Ae. aegypti multiple feeding behavior in DEN transmission in increasing detail and, in so doing, to test questions that are refinements of our current research. Our Progress Report for 3 years of study with Ae. aegypti in Puerto Rico and Thailand indicates that (1) multiple feeding on humans is common, and the time interval between meals may fluctuate in concert with changes in DEN transmission; (2) frequent blood feeding contributes to mosquito energy needs because females rarely feed on plant sugars; and (3) there is geographic variation in entomological factors that may influence vectorial capacity and, thus, virus transmission. Experiments described in this proposal are designed to determine (1) the distribution of multiple meals within a community by identifying the exact person(s) from which Aedes aegypti imbibe blood; (2) nutritional correlates and reproductive fitness associated with multiple feeding behavior; and (3) if mosquito survival is correlated with the frequency of multiple feeding and seasonal dynamics of DEN transmission. information gathered from these studies will be important to people involved in disease prevention through vector control and in basic studies of arboviral ecology and evolution.