RAPID, AUTOMATED DETECTION SYSTEM FOR PCR PRODUCTS

PCR 产品的快速自动化检测系统

• 批准号: 2252910
• 负责人: PAUL D HALE
• 金额: $ 6.93万
• 依托单位: BIO-TEK INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2252910
• 关键词: biomedical automation; biomedical equipment development; biotin; breast neoplasm /cancer diagnosis; chemical kinetics; fluorescent dye /probe; genetic disorder diagnosis; natural gene amplification; neoplasm /cancer genetics; nucleic acid chemical synthesis; nucleic acid probes; nucleic acid sequence; oligonucleotides; personal computers; polymerase chain reaction; protooncogene; rapid diagnosis; synthetic nucleic acid

There is presently a great deal of interest in defecting specific genetic mutations or variants that could provide information about an individual's predisposition to a wide variety of diseases, predict an individual's sensitivity to specific chemicals or drugs, or be used as prognostic indicators for the treatment of patients with diseases such as cancer. Despite the importance of monitoring such genetic variants, present research laboratory techniques are not suitable for routine clinical use, and there is a great need for new, automated, high-throughput diagnostic approaches. We propose to address this problem by developing automated instrumentation for the quantitative detection of specific DNA products generated from DNA or RNA targets by polymerase chain reaction techniques. This computer-controlled instrumentation will be capable of delivering samples and reagents to standard 96-well microplates, maintaining appropriate incubation temperatures for specific hybridization reactions, and performing washing and detection procedures. The proposed instrument will be able to quickly and efficiently process many DNA samples, and the final planned technology will be able to simultaneously screen multiple genes from a single DNA sample.

目前有大量的兴趣在特定的基因缺陷， 突变或变异，可以提供有关个人的信息 易患各种疾病的倾向，预测一个人的 对特定化学品或药物的敏感性，或用作预后 癌症等疾病患者的治疗指标。 尽管监测这些遗传变异的重要性， 研究实验室技术不适合常规临床使用， 并且非常需要新的、自动化的、高通量的诊断 接近。我们建议通过开发自动化的 定量检测特定DNA产物的仪器 通过聚合酶链反应技术从DNA或RNA靶产生。 这种计算机控制的仪器将能够提供 将样品和试剂转移至标准96孔微孔板， 对于特异性杂交反应的适当温育温度， 进行洗涤和检测程序。拟议文书 将能够快速有效地处理许多DNA样本， 最终计划的技术将能够同时筛选多个 一个DNA样本的基因。