SENSORS FOR DIAGNOSIS OF ALCOHOL-RELATED LIVER DAMAGE

用于诊断酒精相关肝损伤的传感器

• 批准号: 3487432
• 负责人: PAUL D HALE
• 金额: $ 5万
• 依托单位: BIO-TEK INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3487432
• 关键词: alanine transaminase; alcoholic beverage consumption; alcoholism /alcohol abuse; aminoacid oxidase; aspartate transaminase; biomarker; biomedical equipment development; biosensor device; blood chemistry; diagnosis design /evaluation; electrical conductance; electrochemistry; electron transport; enzyme electrodes; glutamates; glutamyltransferase; immobilized enzymes; liver disorder diagnosis; monitoring device; pyruvate oxidase; pyruvates

The goals of the Phase l research period are to construct and test new amperometric biosensors for the determination of several serum enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyltransferase (GGT), which are frequently associated with alcohol-related organ damage and alcoholism. The sensors are based on the selective detection of products formed by reactions which are catalyzed by these serum enzymes. A novel sensor design permits efficient operation where interference due to dissolved oxygen and spurious current measurements due to common interferents (e.g., ascorbate, urate) are minimized. During Phase II, this technology will be extended to the selective detection of other laboratory markers of alcoholism, such as creatinine, urea, and glutamate dehydrogenase. The optimized sensors could serve an important role in the daily clinical practice of differential diagnostics of alcohol-related organ damage and in follow-up studies of alcoholism treatment programs.

第一阶段研究阶段的目标是构建和测试新的