IMPROVED GLUCOSE BIOSENSORS

改进的葡萄糖生物传感器

• 批准号: 3496279
• 负责人: PAUL D HALE
• 金额: $ 5万
• 依托单位: BIO-TEK INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1993-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496279
• 关键词: bioengineering /biomedical engineering; biomaterial development /preparation; biomedical equipment development; biosensor device; blood glucose; clinical biomedical equipment; covalent bond; electrochemistry; electron transport; enzyme electrodes; flavins; glucose; glucose oxidase; molecular film; oxidation reduction reaction; patient monitoring device; polymers; water solubility

The proposed work involves the design and testing of new enzyme-based amperometric glucose sensors where electrical communication between the flavin redox centers of glucose oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible, insoluble, high molecular weight polymer. Improvements in stability and selectivity will be pursued through the use of protective polymer coatings and novel enzyme immobilization procedures. The optimized sensors should have widespread technological applications in areas such as the clinical laboratory, veterinary health care, the research laboratory, and the food industry. With the use of protective polymer coatings to prevent fouling of the sensor in biological fluids, and electron transfer mediators which are covalently attached to an insoluble polymer to prevent their diffusion away from the electrode, the development of a reusable in vitro blood glucose monitor (for self-monitoring by diabetic patients) appears feasible. The polymeric relay systems are also amenable to needle-type probes which could be useful for many research applications.

拟议的工作包括设计和测试新的基于酶的 电流型血糖传感器 葡萄糖氧化酶的黄素氧化还原中心和电极是通过 供体-受体继电器的网络化学地绑定到高度灵活的， 不溶于水的高分子量聚合物。提高了稳定性和 将通过使用保护性聚合物来追求选择性 涂层和新的酶固定化程序。经过优化的 传感器应该在以下领域有广泛的技术应用 作为临床实验室、兽医保健、研究 实验室和食品工业。使用保护性聚合物 涂层以防止传感器在生物液体中结垢，以及 电子转移介体共价连接到不溶于水的 聚合物以防止它们从电极上扩散， 一种可重复使用的体外血糖监测仪的研制 糖尿病患者的自我监测)似乎是可行的。聚合体 继电器系统也适用于针型探头，这种探头可能 对许多研究应用程序非常有用。