BIOSENSORS FOR NEUROTRANSMITTER DETECTION

用于神经递质检测的生物传感器

• 批准号: 3504699
• 负责人: PAUL D HALE
• 金额: $ 4.99万
• 依托单位: BIO-TEK INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3504699
• 关键词: acetylcholine; bioengineering /biomedical engineering; biomedical equipment development; biosensor device; chemical synthesis; choline; clinical biomedical equipment; covalent bond; electrochemistry; electron transport; glutamates; implant; microelectrodes; oxidoreductase; polymers

This proposal describes the design and testing of new enzyme-based amperometric sensors for the neurochemically important species, choline, acetylcholine and glutamate. In these devices, the electrical communication between the flavin redox centers of an oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible polymer. Because the electron transfer mediators are covalently attached to an insoluble polymer and cannot diffuse away from the electrode, these sensors should be suitable for implantation, and thus would be useful for in vivo neurochemical research. The proposed research plan concerns the development of highly sensitive and selective micro-biosensors which can be used as implantable probes or in combination with conventional microdialysis techniques. The sensors should permit detection of these neurochemicals over much shorter time scales than presently available assays.

该提案描述了新的基于酶的设计和测试 用于神经化学重要物质胆碱的电流传感器 乙酰胆碱和谷氨酸。 在这些设备中，电 氧化酶和黄素氧化还原中心之间的通讯 电极是通过化学上的供体-受体中继网络实现的 与高柔性聚合物结合。 因为电子转移 介体与不溶性聚合物共价连接，不能 远离电极扩散，这些传感器应该适用于 植入，因此可用于体内神经化学 研究。 拟议的研究计划涉及高度的开发 可用作植入式的灵敏且选择性的微生物传感器 探针或与传统微透析技术相结合。 这 传感器应该能够在更短的时间内检测到这些神经化学物质 时间尺度比目前可用的检测方法要短。