BIOSENSORS FOR NEUROTRANSMITTER DETECTION

本提案描述了新的基于酶的设计和测试 神经化学重要物种胆碱的安培传感器， 乙酰胆碱和谷氨酸。在这些设备中，电子设备 黄素氧化还原中心与氧化物酶之间的通讯 电极是通过化学上的供体-受体继电器网络实现的。 结合到高度柔韧的聚合物上。因为电子转移 介体以共价方式连接到不溶于水的聚合物上，不能 从电极扩散开，这些传感器应该适用于 植入，因此将用于体内神经化学 研究。拟议的研究计划涉及到高度 可作为植入型的灵敏和选择性的微型生物传感器 探头或与常规微渗析技术相结合。这个 传感器应该能够在短得多的时间内检测到这些神经化学物质 时间尺度比目前可用的分析方法更短。

This proposal describes the design and testing of new enzyme-based amperometric sensors for the neurochemically important species, choline, acetylcholine and glutamate. In these devices, the electrical communication between the flavin redox centers of an oxidase and an electrode is achieved via a network of donor-acceptor relays chemically bound to a highly flexible polymer. Because the electron transfer mediators are covalently attached to an insoluble polymer and cannot diffuse away from the electrode, these sensors should be suitable for implantation, and thus would be useful for in vivo neurochemical research. The proposed research plan concerns the development of highly sensitive and selective micro-biosensors which can be used as implantable probes or in combination with conventional microdialysis techniques. The sensors should permit detection of these neurochemicals over much shorter time scales than presently available assays.