ELECTROCHEMICAL SENSORS FOR ENZYME IMMUNOASSAYS

用于酶免疫测定的电化学传感器

• 批准号: 3498818
• 负责人: PAUL D HALE
• 金额: $ 4.99万
• 依托单位: BIO-TEK INSTRUMENTS, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-15 至 1993-10-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498818
• 关键词: biosensor device; electrochemistry; electrodes; immunologic assay /test; method development; nicotinamide adenine dinucleotide; polymers

The overall objective of this project is to develop highly stable electrochemical probes for the monitoring of the reduced cofactor nicotinamide adenine dinucleotide (NADH), which is produced in many enzyme-based immunoassays. These sensors can measure NADH concentrations at low applied potentials, where electrochemical interferences and deactivating chemical reactions are avoided. The Phase I research will attempt to establish the feasibility of using these new sensors for the measurement of NADH produced in clinically important immunoassays. The development of an electrochemical probe which can selectively measure NADH concentrations could permit analysis of very small samples, which is of importance, for example, to clinical assays of neonatal blood samples. During the Phase II program, the construction and testing of small sample chambers (e.g., "capillary" immunoassays), for use with these electrochemical sensors, could lead to the development of rapid, automated immunoassays requiring very small amounts of sample.

该项目的总体目标是开发高度稳定的 用于监测还原辅因子的电化学探针 烟酰胺腺嘌呤二核苷酸（NADH），它是在许多 基于酶的免疫测定。 这些传感器可以测量NADH浓度 在低应用电位，其中电化学干扰和 避免了失活化学反应。 第一阶段研究将 试图建立使用这些新传感器的可行性， 在临床上重要的免疫测定中产生的NADH的测量。 的 电化学探针的研制 NADH浓度可以允许分析非常小的样品， 例如，对于新生儿血液的临床分析是重要的 样品 在第二阶段计划中， 较小的样品室（例如，"毛细管"免疫测定），用于与 这些电化学传感器，可以导致快速， 自动化免疫测定需要非常少量的样品。