GASTRIC FUNCTION--TRH AND 5HT IN CAUDAL RAPHE CONTROL

描述：TRH和5HT共同定位于尾侧投射。 中缝核向迷走神经背侧复合体(DVC)核投射。促甲状腺激素释放激素和5羟色胺 协同作用似乎参与了胃的迷走控制。 功能。将进行选择性5-羟色胺拮抗剂的微量注射 以确定参与DVC的5-羟色胺受体亚型 协同反应。大鼠延髓背侧5-羟色胺的释放 并将禁食动物与记录的5-羟色胺释放进行比较 经尾侧中缝核投射在酸性增强中起关键作用 餐后分泌物和运动能力。兴奋性的可能作用 迷走神经传入中的氨基酸和P物质作为神经递质 餐后胃刺激反应的中介作用将被检测 使用P物质的激动剂和拮抗剂 谷氨酸。还将进行研究以确定DVC神经元(使用 细胞外记录)，由胃扩张激活。 促肾上腺皮质激素释放激素和5-羟色胺微量注射的作用及其协同作用 直接到孤束核或DVC核上的“胃反应” DVC神经元“将被检查。

DESCRIPTION: TRH and 5HT are colocalized in the projections of caudal raphe nuclei onto dorsal vagal complex (DVC) nuclei. TRH and 5HT synergism appears to be involved in the vagal control of gastric function. Microinjections of selective 5HT antagonists will be performed in DVC in order to identify the 5HT receptor subtype involved in the synergistic response. Serotonin release in the dorsal medulla of fed and fasting animals will be compared to document that serotonin release by caudal raphe nuclei projections plays a key role in enhanced acid secretion and motility after a meal. A possible role of excitatory amino acids and substance P as neurotransmitters in the vagal afferents which mediate gastric stimulatory response after a meal will be examined with the use of the agonists and the antagonists of substance P and glutamate. Studies will also be performed to identify DVC neurons (using extracellular recordings) which are activated by gastric distension. Effects and synergistic interactions of TRH and 5HT microinjection directly onto solitary nucleus or DVC nuclei on the "gastric responsive DVC neurons" will be examined.